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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Bioorganic chemistry
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41719918/ |
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| _version_ | 1868266082227191808 |
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| author | Yang, Fanghao Xu, Fenghua Zhang, Yun Zhang, Huhu Wen, Ziyuan Su, Mohan Yu, Chunjuan Wang, Yiming Yang, Lina Shi, Chunxiao Xin, Maosheng Zhang, Shanshan Li, Bing |
| author_facet | Yang, Fanghao Xu, Fenghua Zhang, Yun Zhang, Huhu Wen, Ziyuan Su, Mohan Yu, Chunjuan Wang, Yiming Yang, Lina Shi, Chunxiao Xin, Maosheng Zhang, Shanshan Li, Bing Yang, Fanghao Xu, Fenghua Zhang, Yun Zhang, Huhu Wen, Ziyuan Su, Mohan Yu, Chunjuan Wang, Yiming Yang, Lina Shi, Chunxiao Xin, Maosheng Zhang, Shanshan Li, Bing |
| collection | PubMed - marine biology |
| contents | Multi-omics integrative analysis elucidates the anti-inflammatory effects and underlying mechanisms of Arthrospira platensis-derived peptides in ulcerative colitis. Yang, Fanghao Xu, Fenghua Zhang, Yun Zhang, Huhu Wen, Ziyuan Su, Mohan Yu, Chunjuan Wang, Yiming Yang, Lina Shi, Chunxiao Xin, Maosheng Zhang, Shanshan Li, Bing Animals Colitis, Ulcerative Mice Multiomics Mice, Inbred C57BL Peptides Spirulina Oxidative Stress Male Anti-Inflammatory Agents Anti-Inflammatory Agents, Non-Steroidal Gastrointestinal Microbiome Dextran Sulfate Disease Models, Animal Inflammatory bowel disease (IBD), particularly ulcerative colitis (UC), remains a global health challenge due to limited therapeutic efficacy and adverse effects of current treatments. Marine-derived peptides, recognized for their structural diversity and bioactivity, hold promise as novel therapeutics. Arthrospira platensis-derived peptide P1 has demonstrated anti-inflammatory potential, warranting exploration of its mechanisms in UC. This study aimed to investigate the therapeutic effects and molecular mechanisms of P1 in a DSS-induced UC mouse model using multi-omics integration. UC was induced in C57BL/6 J mice via 3.5% DSS. P1 or 5-ASA was administered for 7 days. Systemic analyses included gut microbiota profiling (16S rRNA sequencing), transcriptomics, proteomics, histopathology, oxidative stress assays, RT-qPCR, and Western blotting. P1 restored gut microbiota diversity, suppressing Akkermansia while enriching Rikenellaceae and Prevotella. It attenuated oxidative stress by reducing ROS/MDA and enhancing SOD/CAT/GSH-Px activity, mitigated apoptosis via TUNEL assay, and repaired epithelial barrier integrity by upregulating ZO-1/Occludin. Multi-omics integration revealed P1's dual regulation of PPAR and NF-κB pathways: PPARγ activation upregulated lipid metabolism genes (pparγ, hmgcs1) and suppressed NF-κB signaling (nf-κb, tnf-α, il-1β), validated by reduced p-P65 and iNOS alongside elevated IκBα. P1 downregulated pro-inflammatory cytokines (TNF-α, IL-6, IL-17) and increased IL-10. These results showed that P1 alleviated UC through microbiota modulation, oxidative stress reduction, barrier restoration, and PPAR/NF-κB crosstalk regulation, positioning it as a multi-target therapeutic candidate for IBD. This study provides a mechanistic foundation for developing peptide-based strategies in precision colitis management. |
| format | Artículo científico |
| id | pubmed_41719918 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Bioorganic chemistry |
| record_format | pubmed |
| spellingShingle | Multi-omics integrative analysis elucidates the anti-inflammatory effects and underlying mechanisms of Arthrospira platensis-derived peptides in ulcerative colitis. Yang, Fanghao Xu, Fenghua Zhang, Yun Zhang, Huhu Wen, Ziyuan Su, Mohan Yu, Chunjuan Wang, Yiming Yang, Lina Shi, Chunxiao Xin, Maosheng Zhang, Shanshan Li, Bing Animals Colitis, Ulcerative Mice Multiomics Mice, Inbred C57BL Peptides Spirulina Oxidative Stress Male Anti-Inflammatory Agents Anti-Inflammatory Agents, Non-Steroidal Gastrointestinal Microbiome Dextran Sulfate Disease Models, Animal Multi-omics integrative analysis elucidates the anti-inflammatory effects and underlying mechanisms of Arthrospira platensis-derived peptides in ulcerative colitis. Yang, Fanghao Xu, Fenghua Zhang, Yun Zhang, Huhu Wen, Ziyuan Su, Mohan Yu, Chunjuan Wang, Yiming Yang, Lina Shi, Chunxiao Xin, Maosheng Zhang, Shanshan Li, Bing Animals Colitis, Ulcerative Mice Multiomics Mice, Inbred C57BL Peptides Spirulina Oxidative Stress Male Anti-Inflammatory Agents Anti-Inflammatory Agents, Non-Steroidal Gastrointestinal Microbiome Dextran Sulfate Disease Models, Animal Inflammatory bowel disease (IBD), particularly ulcerative colitis (UC), remains a global health challenge due to limited therapeutic efficacy and adverse effects of current treatments. Marine-derived peptides, recognized for their structural diversity and bioactivity, hold promise as novel therapeutics. Arthrospira platensis-derived peptide P1 has demonstrated anti-inflammatory potential, warranting exploration of its mechanisms in UC. This study aimed to investigate the therapeutic effects and molecular mechanisms of P1 in a DSS-induced UC mouse model using multi-omics integration. UC was induced in C57BL/6 J mice via 3.5% DSS. P1 or 5-ASA was administered for 7 days. Systemic analyses included gut microbiota profiling (16S rRNA sequencing), transcriptomics, proteomics, histopathology, oxidative stress assays, RT-qPCR, and Western blotting. P1 restored gut microbiota diversity, suppressing Akkermansia while enriching Rikenellaceae and Prevotella. It attenuated oxidative stress by reducing ROS/MDA and enhancing SOD/CAT/GSH-Px activity, mitigated apoptosis via TUNEL assay, and repaired epithelial barrier integrity by upregulating ZO-1/Occludin. Multi-omics integration revealed P1's dual regulation of PPAR and NF-κB pathways: PPARγ activation upregulated lipid metabolism genes (pparγ, hmgcs1) and suppressed NF-κB signaling (nf-κb, tnf-α, il-1β), validated by reduced p-P65 and iNOS alongside elevated IκBα. P1 downregulated pro-inflammatory cytokines (TNF-α, IL-6, IL-17) and increased IL-10. These results showed that P1 alleviated UC through microbiota modulation, oxidative stress reduction, barrier restoration, and PPAR/NF-κB crosstalk regulation, positioning it as a multi-target therapeutic candidate for IBD. This study provides a mechanistic foundation for developing peptide-based strategies in precision colitis management. |
| title | Multi-omics integrative analysis elucidates the anti-inflammatory effects and underlying mechanisms of Arthrospira platensis-derived peptides in ulcerative colitis. |
| topic | Animals Colitis, Ulcerative Mice Multiomics Mice, Inbred C57BL Peptides Spirulina Oxidative Stress Male Anti-Inflammatory Agents Anti-Inflammatory Agents, Non-Steroidal Gastrointestinal Microbiome Dextran Sulfate Disease Models, Animal |
| url | https://pubmed.ncbi.nlm.nih.gov/41719918/ |