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| Autores principales: | , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Biology
2026
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| Acceso en línea: | https://pubmed.ncbi.nlm.nih.gov/41744621/ |
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| _version_ | 1868266079703269377 |
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| author | Hou, Di Bao, Yuhao Chen, Yuxiong Zhou, Qi Zhu, Xiaoyu Xie, Xi Zhu, Dongfa |
| author_facet | Hou, Di Bao, Yuhao Chen, Yuxiong Zhou, Qi Zhu, Xiaoyu Xie, Xi Zhu, Dongfa Hou, Di Bao, Yuhao Chen, Yuxiong Zhou, Qi Zhu, Xiaoyu Xie, Xi Zhu, Dongfa |
| collection | PubMed - marine biology |
| contents | Molecular Identification of in the Swimming Crab and Its Potential Involvement in Ovarian Development. Hou, Di Bao, Yuhao Chen, Yuxiong Zhou, Qi Zhu, Xiaoyu Xie, Xi Zhu, Dongfa Nuclear receptor is considered as a non-insect arthropod-specific receptor, but its roles in decapod reproduction remain poorly understood. Here, we identified and characterized an ortholog from the swimming crab () and verified its placement within the NR1L nuclear receptor family by phylogenetic analysis. encodes a canonical nuclear receptor with a conserved DNA-binding domain (DBD) and ligand-binding domain (LBD). Quantitative PCR revealed predominant expression in the ovary and stage-dependent changes during ovarian development. Using an ovarian explant culture system, we found that arachidonic acid (AA) consistently suppressed transcript levels, while methyl farnesoate (MF) and pyriproxyfen (P) had no significant effect, indicating a potential inhibitory role for AA in expression. RNA interference of caused significant changes in ovarian development, including reduced GSI, smaller oocytes, and uneven eosinophilic granule distribution. Transcriptomic profiling of HR97-silenced ovaries indicated that the major responses involved genes associated with substrate transport/exchange, cell boundary-related signaling and transduction, and disturbed nuclear transcriptional regulation. Short-term in vivo perturbations ( RNAi and AA treatment) further supported these expression changes and revealed that AA- and RNAi-elicited transcriptional responses only partially overlapped. Taken together, these results suggest that contributes to ovarian development, potentially through broad transcriptional responses related to transport, signaling, and gene regulation. Although AA may suppress expression, does not fully explain AA-mediated regulation of ovarian development. |
| format | Artículo científico |
| id | pubmed_41744621 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Biology |
| record_format | pubmed |
| spellingShingle | Molecular Identification of in the Swimming Crab and Its Potential Involvement in Ovarian Development. Hou, Di Bao, Yuhao Chen, Yuxiong Zhou, Qi Zhu, Xiaoyu Xie, Xi Zhu, Dongfa Molecular Identification of in the Swimming Crab and Its Potential Involvement in Ovarian Development. Hou, Di Bao, Yuhao Chen, Yuxiong Zhou, Qi Zhu, Xiaoyu Xie, Xi Zhu, Dongfa Nuclear receptor is considered as a non-insect arthropod-specific receptor, but its roles in decapod reproduction remain poorly understood. Here, we identified and characterized an ortholog from the swimming crab () and verified its placement within the NR1L nuclear receptor family by phylogenetic analysis. encodes a canonical nuclear receptor with a conserved DNA-binding domain (DBD) and ligand-binding domain (LBD). Quantitative PCR revealed predominant expression in the ovary and stage-dependent changes during ovarian development. Using an ovarian explant culture system, we found that arachidonic acid (AA) consistently suppressed transcript levels, while methyl farnesoate (MF) and pyriproxyfen (P) had no significant effect, indicating a potential inhibitory role for AA in expression. RNA interference of caused significant changes in ovarian development, including reduced GSI, smaller oocytes, and uneven eosinophilic granule distribution. Transcriptomic profiling of HR97-silenced ovaries indicated that the major responses involved genes associated with substrate transport/exchange, cell boundary-related signaling and transduction, and disturbed nuclear transcriptional regulation. Short-term in vivo perturbations ( RNAi and AA treatment) further supported these expression changes and revealed that AA- and RNAi-elicited transcriptional responses only partially overlapped. Taken together, these results suggest that contributes to ovarian development, potentially through broad transcriptional responses related to transport, signaling, and gene regulation. Although AA may suppress expression, does not fully explain AA-mediated regulation of ovarian development. |
| title | Molecular Identification of in the Swimming Crab and Its Potential Involvement in Ovarian Development. |
| url | https://pubmed.ncbi.nlm.nih.gov/41744621/ |