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| Hauptverfasser: | , , , , , , , , , , , |
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| Format: | Artículo científico |
| Sprache: | en |
| Veröffentlicht: |
Animals : an open access journal from MDPI
2026
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/41751102/ |
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Inhaltsangabe:
- Multiplex Species-Specific PCR Identification of Native Onchidiid Slugs in the South China Sea: A Useful Tool for Application in Onchidiid Slug Stock Management and Germplasm Conservation. Huang, Jingyue Ma, Haitao Duan, Xixi Wei, Zonglu Zhang, Yinjie Zhang, Tao Chen, Xiaoqun Qin, Yanping Li, Jun Yu, Ziniu Pan, Ying Zhang, Yuehuan To address the need for accurate onchidiid slug species identification, the present study engineered a multiplex PCR-based assay to facilitate the efficient, economical, and discrimination of five morphologically similar onchidiid slug species endemic to the South China Sea: , , , and . A diagnostic multiplex PCR assay was designed based on a partial fragment of the mitochondrial cytochrome oxidase subunit I. It utilizes a shared forward primer and five taxon-specific reverse primers to simultaneously produce amplicons of varying sizes, each indicative of a particular species. Five species of Onchidiidae can be identified in a single reaction, which provides technical support for molecular identification of Onchidiidae. The development of a simple, effective and rapid multiple PCR identification method is of great significance for stock management and germplasm conservation of Onchidiidae.