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| Main Authors: | , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Microorganisms
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41753615/ |
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| _version_ | 1868266079690686464 |
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| author | Nedashkovskaya, Olga Bystritskaya, Evgeniya Savicheva, Yulia Bronnikova, Yulia Otstavnykh, Nadezhda Eremeev, Viacheslav Kim, Song-Gun Zhukova, Natalia Isaeva, Marina |
| author_facet | Nedashkovskaya, Olga Bystritskaya, Evgeniya Savicheva, Yulia Bronnikova, Yulia Otstavnykh, Nadezhda Eremeev, Viacheslav Kim, Song-Gun Zhukova, Natalia Isaeva, Marina Nedashkovskaya, Olga Bystritskaya, Evgeniya Savicheva, Yulia Bronnikova, Yulia Otstavnykh, Nadezhda Eremeev, Viacheslav Kim, Song-Gun Zhukova, Natalia Isaeva, Marina |
| collection | PubMed - marine biology |
| contents | Genome Analysis and Characterization of sp. nov. Isolated from Brown and Green Algae, and a Proposal to Reclassify Cao et al. 2020 and Li et al. 2015 as New Members. Nedashkovskaya, Olga Bystritskaya, Evgeniya Savicheva, Yulia Bronnikova, Yulia Otstavnykh, Nadezhda Eremeev, Viacheslav Kim, Song-Gun Zhukova, Natalia Isaeva, Marina Two marine bacteria, designated strains 4Alg 33and 3Alg 14/1, were isolated from brown () and green () macroalgae, respectively. These isolates were aerobic Gram-negative rods exhibiting a gliding motility. The 16S rRNA gene phylogenetic analysis clearly showed their belonging to the genus , the family , and the phylum . The closest relatives of the new strains were KCTC 32328 (99.05%), IMCC 9485 (99.05%) and KMM 3901 (98.96%). The ANI and dDDH values between the two new strains were 97.9% and 85.3%, respectively. The AAI values between 4Alg 33 and type strains ranged from 80.1% ( MA1) to 91.4% ( KCTC 32328). The cellular fatty acid and polar lipid profiles of the new isolates were generally similar to those of the type strains of species. The genomes of 4Alg 33 and 3Alg 14/1 are represented by a circular chromosome of 4,157,724 bp and 4,316,096 bp in size with 3536 and 3879 protein-coding genes, respectively. They shared a DNA G+C content of 34.3 mol% and comprised four operons. The pangenome of the genus belongs to the open type and is characterized by an abundance of CAZymes. The proportion of CAZyme genes in novel genomes was more than 5%, with a prevalence of glycoside hydrolase genes, suggesting great potential for utilizing marine-derived polysaccharides. Based on the results of polyphasic characterization, the two algal isolates represent a distinct species lineage within the genus , for which we propose the name sp. nov. with the type strain 4Alg 33 (= KMM 3963 = KCTC 72008). In addition, we have proposed to transfer Cao et al. 2020 and Li et al. 2015 to the genus Vaidya et al. 2015 as comb. nov. and comb. nov. based on a combination of the genomic and phenotypic characteristics. |
| format | Artículo científico |
| id | pubmed_41753615 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Microorganisms |
| record_format | pubmed |
| spellingShingle | Genome Analysis and Characterization of sp. nov. Isolated from Brown and Green Algae, and a Proposal to Reclassify Cao et al. 2020 and Li et al. 2015 as New Members. Nedashkovskaya, Olga Bystritskaya, Evgeniya Savicheva, Yulia Bronnikova, Yulia Otstavnykh, Nadezhda Eremeev, Viacheslav Kim, Song-Gun Zhukova, Natalia Isaeva, Marina Genome Analysis and Characterization of sp. nov. Isolated from Brown and Green Algae, and a Proposal to Reclassify Cao et al. 2020 and Li et al. 2015 as New Members. Nedashkovskaya, Olga Bystritskaya, Evgeniya Savicheva, Yulia Bronnikova, Yulia Otstavnykh, Nadezhda Eremeev, Viacheslav Kim, Song-Gun Zhukova, Natalia Isaeva, Marina Two marine bacteria, designated strains 4Alg 33and 3Alg 14/1, were isolated from brown () and green () macroalgae, respectively. These isolates were aerobic Gram-negative rods exhibiting a gliding motility. The 16S rRNA gene phylogenetic analysis clearly showed their belonging to the genus , the family , and the phylum . The closest relatives of the new strains were KCTC 32328 (99.05%), IMCC 9485 (99.05%) and KMM 3901 (98.96%). The ANI and dDDH values between the two new strains were 97.9% and 85.3%, respectively. The AAI values between 4Alg 33 and type strains ranged from 80.1% ( MA1) to 91.4% ( KCTC 32328). The cellular fatty acid and polar lipid profiles of the new isolates were generally similar to those of the type strains of species. The genomes of 4Alg 33 and 3Alg 14/1 are represented by a circular chromosome of 4,157,724 bp and 4,316,096 bp in size with 3536 and 3879 protein-coding genes, respectively. They shared a DNA G+C content of 34.3 mol% and comprised four operons. The pangenome of the genus belongs to the open type and is characterized by an abundance of CAZymes. The proportion of CAZyme genes in novel genomes was more than 5%, with a prevalence of glycoside hydrolase genes, suggesting great potential for utilizing marine-derived polysaccharides. Based on the results of polyphasic characterization, the two algal isolates represent a distinct species lineage within the genus , for which we propose the name sp. nov. with the type strain 4Alg 33 (= KMM 3963 = KCTC 72008). In addition, we have proposed to transfer Cao et al. 2020 and Li et al. 2015 to the genus Vaidya et al. 2015 as comb. nov. and comb. nov. based on a combination of the genomic and phenotypic characteristics. |
| title | Genome Analysis and Characterization of sp. nov. Isolated from Brown and Green Algae, and a Proposal to Reclassify Cao et al. 2020 and Li et al. 2015 as New Members. |
| url | https://pubmed.ncbi.nlm.nih.gov/41753615/ |