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Autori principali: Zhang, Jiansong, Yang, Yuqing, Chang, Jingwen, Ding, Jingfeng, Tang, Shiqi, Lu, Yishan, Wei, Xiumei, Yang, Jialong
Natura: Artículo científico
Lingua:en
Pubblicazione: Fish & shellfish immunology 2026
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Accesso online:https://pubmed.ncbi.nlm.nih.gov/41786104/
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author Zhang, Jiansong
Yang, Yuqing
Chang, Jingwen
Ding, Jingfeng
Tang, Shiqi
Lu, Yishan
Wei, Xiumei
Yang, Jialong
author_facet Zhang, Jiansong
Yang, Yuqing
Chang, Jingwen
Ding, Jingfeng
Tang, Shiqi
Lu, Yishan
Wei, Xiumei
Yang, Jialong
Zhang, Jiansong
Yang, Yuqing
Chang, Jingwen
Ding, Jingfeng
Tang, Shiqi
Lu, Yishan
Wei, Xiumei
Yang, Jialong
collection PubMed - marine biology
contents Dietary ferulic acid boosts the immunity and antioxidant activity of T lymphocytes in Nile tilapia (Oreochromis niloticus) challenged with Aeromonas hydrophila. Zhang, Jiansong Yang, Yuqing Chang, Jingwen Ding, Jingfeng Tang, Shiqi Lu, Yishan Wei, Xiumei Yang, Jialong Animals Coumaric Acids Aeromonas hydrophila Cichlids Gram-Negative Bacterial Infections Diet Dietary Supplements Animal Feed Antioxidants Fish Diseases T-Lymphocytes Dose-Response Relationship, Drug Immunonutrition Diet Adaptive Immunity Random Allocation Functional feed additives are increasingly incorporated into aquafeeds to improve growth performance, metabolic status, and overall health of farmed fish. However, their regulatory effects on adaptive immunity, particularly T cell-mediated immune responses, remain poorly characterized. In this study, we evaluated the effects of dietary ferulic acid supplementation on T cell immunity and antioxidant capacity in Nile tilapia (Oreochromis niloticus). Fish were fed diets supplemented with 0, 200, or 400 mg/kg ferulic acid and subsequently challenged with Aeromonas hydrophila. Compared with the basal diet group, supplementation with 200 mg/kg ferulic acid significantly enhanced T cell proliferation and survival during infection, accompanied by increased proportions and absolute numbers of CD4-1 T cells. In parallel, ferulic acid supplementation elevated expression of key T cell functional molecules, including interferon-γ (IFN-γ), perforin A, tumor necrosis factor α (TNF-α), and Fas ligand (Fas-L), together with reduced bacterial burden, alleviated hepatic inflammatory infiltration and lesions, and improved survival. In addition, ferulic acid markedly enhanced antioxidant capacity in lymphocytes, as evidenced by increased activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px), elevated total antioxidant capacity (T-AOC), and reduced malondialdehyde (MDA) levels. Consistently, intracellular reactive oxygen species (ROS) accumulation in T cells and the expression of pro-inflammatory genes were significantly reduced following 200 mg/kg ferulic acid supplementation. Taken together, we demonstrate that dietary ferulic acid supplementation functionally enhances T cell-mediated immune responses and antioxidant capacity in tilapia during bacterial infection, with 200 mg/kg identified as the optimal concentration, supporting its potential application as a functional feed additive in aquaculture.
format Artículo científico
id pubmed_41786104
institution PubMed
language en
publishDate 2026
publisher Fish & shellfish immunology
record_format pubmed
spellingShingle Dietary ferulic acid boosts the immunity and antioxidant activity of T lymphocytes in Nile tilapia (Oreochromis niloticus) challenged with Aeromonas hydrophila.
Zhang, Jiansong
Yang, Yuqing
Chang, Jingwen
Ding, Jingfeng
Tang, Shiqi
Lu, Yishan
Wei, Xiumei
Yang, Jialong
Animals
Coumaric Acids
Aeromonas hydrophila
Cichlids
Gram-Negative Bacterial Infections
Diet
Dietary Supplements
Animal Feed
Antioxidants
Fish Diseases
T-Lymphocytes
Dose-Response Relationship, Drug
Immunonutrition Diet
Adaptive Immunity
Random Allocation
Dietary ferulic acid boosts the immunity and antioxidant activity of T lymphocytes in Nile tilapia (Oreochromis niloticus) challenged with Aeromonas hydrophila. Zhang, Jiansong Yang, Yuqing Chang, Jingwen Ding, Jingfeng Tang, Shiqi Lu, Yishan Wei, Xiumei Yang, Jialong Animals Coumaric Acids Aeromonas hydrophila Cichlids Gram-Negative Bacterial Infections Diet Dietary Supplements Animal Feed Antioxidants Fish Diseases T-Lymphocytes Dose-Response Relationship, Drug Immunonutrition Diet Adaptive Immunity Random Allocation Functional feed additives are increasingly incorporated into aquafeeds to improve growth performance, metabolic status, and overall health of farmed fish. However, their regulatory effects on adaptive immunity, particularly T cell-mediated immune responses, remain poorly characterized. In this study, we evaluated the effects of dietary ferulic acid supplementation on T cell immunity and antioxidant capacity in Nile tilapia (Oreochromis niloticus). Fish were fed diets supplemented with 0, 200, or 400 mg/kg ferulic acid and subsequently challenged with Aeromonas hydrophila. Compared with the basal diet group, supplementation with 200 mg/kg ferulic acid significantly enhanced T cell proliferation and survival during infection, accompanied by increased proportions and absolute numbers of CD4-1 T cells. In parallel, ferulic acid supplementation elevated expression of key T cell functional molecules, including interferon-γ (IFN-γ), perforin A, tumor necrosis factor α (TNF-α), and Fas ligand (Fas-L), together with reduced bacterial burden, alleviated hepatic inflammatory infiltration and lesions, and improved survival. In addition, ferulic acid markedly enhanced antioxidant capacity in lymphocytes, as evidenced by increased activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px), elevated total antioxidant capacity (T-AOC), and reduced malondialdehyde (MDA) levels. Consistently, intracellular reactive oxygen species (ROS) accumulation in T cells and the expression of pro-inflammatory genes were significantly reduced following 200 mg/kg ferulic acid supplementation. Taken together, we demonstrate that dietary ferulic acid supplementation functionally enhances T cell-mediated immune responses and antioxidant capacity in tilapia during bacterial infection, with 200 mg/kg identified as the optimal concentration, supporting its potential application as a functional feed additive in aquaculture.
title Dietary ferulic acid boosts the immunity and antioxidant activity of T lymphocytes in Nile tilapia (Oreochromis niloticus) challenged with Aeromonas hydrophila.
topic Animals
Coumaric Acids
Aeromonas hydrophila
Cichlids
Gram-Negative Bacterial Infections
Diet
Dietary Supplements
Animal Feed
Antioxidants
Fish Diseases
T-Lymphocytes
Dose-Response Relationship, Drug
Immunonutrition Diet
Adaptive Immunity
Random Allocation
url https://pubmed.ncbi.nlm.nih.gov/41786104/