From Computer to Sensor: A Proof-of-Concept Aptamer Platform Combining Design, Optical Validation, and Electrochemical Detection of the Fish Allergen -Parvalbumin.
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| Main Authors: | , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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ACS sensors
2026
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| _version_ | 1868266076987457536 |
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| author | Abril, Ana G Sánchez-Visedo, Adrián Pazos, Manuel Morsch, Flemming Poulos, Georgios Code, Christian Prado, Marta Carrera, Mónica Campos, Rui |
| author_facet | Abril, Ana G Sánchez-Visedo, Adrián Pazos, Manuel Morsch, Flemming Poulos, Georgios Code, Christian Prado, Marta Carrera, Mónica Campos, Rui Abril, Ana G Sánchez-Visedo, Adrián Pazos, Manuel Morsch, Flemming Poulos, Georgios Code, Christian Prado, Marta Carrera, Mónica Campos, Rui |
| collection | PubMed - marine biology |
| contents | From Computer to Sensor: A Proof-of-Concept Aptamer Platform Combining Design, Optical Validation, and Electrochemical Detection of the Fish Allergen -Parvalbumin. Abril, Ana G Sánchez-Visedo, Adrián Pazos, Manuel Morsch, Flemming Poulos, Georgios Code, Christian Prado, Marta Carrera, Mónica Campos, Rui Aptamers, Nucleotide Allergens Biosensing Techniques Animals Electrochemical Techniques Fishes Parvalbumins Limit of Detection Computer Simulation Seafood Fish Proteins Proof of Concept Study Ensuring food safety is a critical concern in the modern food industry, as the presence of allergens, toxins, and pathogens can pose significant risks to consumer health. Beta-parvalbumin (β-PRVB) is a calcium-binding protein and the main fish allergen present in raw and processed food products, triggering severe allergic reactions in sensitive individuals. In this research paper, we explore the development of an electrochemical aptamer-based biosensor for the detection of β-PRVB. The biosensor development began with the design of aptamers targeting β-PRVB, which were assessed for their docking and binding capabilities. This was followed by validation using a fluorescence quenching assay, ultimately leading to the creation of an electrochemical aptamer-based sensor utilizing the selected aptamer. The sensor showed a very low limit of detection (LOD) of 3.3 μg/mL and a limit of quantification (LOQ) of 10 μg/mL, with its specificity being tested with beta-lactoglobulin (β-Lact). Samples of commercial processed seafood products were analyzed to determine the effectiveness of the sensor, and the results were validated. |
| format | Artículo científico |
| id | pubmed_41790578 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | ACS sensors |
| record_format | pubmed |
| spellingShingle | From Computer to Sensor: A Proof-of-Concept Aptamer Platform Combining Design, Optical Validation, and Electrochemical Detection of the Fish Allergen -Parvalbumin. Abril, Ana G Sánchez-Visedo, Adrián Pazos, Manuel Morsch, Flemming Poulos, Georgios Code, Christian Prado, Marta Carrera, Mónica Campos, Rui Aptamers, Nucleotide Allergens Biosensing Techniques Animals Electrochemical Techniques Fishes Parvalbumins Limit of Detection Computer Simulation Seafood Fish Proteins Proof of Concept Study From Computer to Sensor: A Proof-of-Concept Aptamer Platform Combining Design, Optical Validation, and Electrochemical Detection of the Fish Allergen -Parvalbumin. Abril, Ana G Sánchez-Visedo, Adrián Pazos, Manuel Morsch, Flemming Poulos, Georgios Code, Christian Prado, Marta Carrera, Mónica Campos, Rui Aptamers, Nucleotide Allergens Biosensing Techniques Animals Electrochemical Techniques Fishes Parvalbumins Limit of Detection Computer Simulation Seafood Fish Proteins Proof of Concept Study Ensuring food safety is a critical concern in the modern food industry, as the presence of allergens, toxins, and pathogens can pose significant risks to consumer health. Beta-parvalbumin (β-PRVB) is a calcium-binding protein and the main fish allergen present in raw and processed food products, triggering severe allergic reactions in sensitive individuals. In this research paper, we explore the development of an electrochemical aptamer-based biosensor for the detection of β-PRVB. The biosensor development began with the design of aptamers targeting β-PRVB, which were assessed for their docking and binding capabilities. This was followed by validation using a fluorescence quenching assay, ultimately leading to the creation of an electrochemical aptamer-based sensor utilizing the selected aptamer. The sensor showed a very low limit of detection (LOD) of 3.3 μg/mL and a limit of quantification (LOQ) of 10 μg/mL, with its specificity being tested with beta-lactoglobulin (β-Lact). Samples of commercial processed seafood products were analyzed to determine the effectiveness of the sensor, and the results were validated. |
| title | From Computer to Sensor: A Proof-of-Concept Aptamer Platform Combining Design, Optical Validation, and Electrochemical Detection of the Fish Allergen -Parvalbumin. |
| topic | Aptamers, Nucleotide Allergens Biosensing Techniques Animals Electrochemical Techniques Fishes Parvalbumins Limit of Detection Computer Simulation Seafood Fish Proteins Proof of Concept Study |
| url | https://pubmed.ncbi.nlm.nih.gov/41790578/ |