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Autores principales: Zhang, Xiaoyue, Li, Pengwei, Niu, Suhao, Gao, Kun, Sun, Mengsi, Wu, Yujie, Bi, Yunchen
Formato: Artículo científico
Lenguaje:en
Publicado: Biochemical and biophysical research communications 2026
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Acceso en línea:https://pubmed.ncbi.nlm.nih.gov/41795371/
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author Zhang, Xiaoyue
Li, Pengwei
Niu, Suhao
Gao, Kun
Sun, Mengsi
Wu, Yujie
Bi, Yunchen
author_facet Zhang, Xiaoyue
Li, Pengwei
Niu, Suhao
Gao, Kun
Sun, Mengsi
Wu, Yujie
Bi, Yunchen
Zhang, Xiaoyue
Li, Pengwei
Niu, Suhao
Gao, Kun
Sun, Mengsi
Wu, Yujie
Bi, Yunchen
collection PubMed - marine biology
contents An acetyl-dependent proofreading mechanism governs synthetic fidelity in Phytophthora sojae chitin synthase. Zhang, Xiaoyue Li, Pengwei Niu, Suhao Gao, Kun Sun, Mengsi Wu, Yujie Bi, Yunchen Chitin Synthase Phytophthora Molecular Dynamics Simulation Cryoelectron Microscopy Chitin Substrate Specificity Chitin is an essential structural polysaccharide in fungal and oomycete cell walls, providing mechanical strength and integrity due to its crystalline, insoluble nature. Chitin synthase (CHS) is the key enzyme responsible for its biosynthesis, orchestrating the sequential steps of substrate binding, hydrolysis, glycosidic bond formation, and polymer translocation. However, the molecular mechanism that governs its synthetic fidelity remains unclear. To address this, we investigated whether Phytophthora sojae chitin synthase 1 (PsChs1) can utilize UDP-glucosamine (UDP-GlcN), a deacetylated substrate analog that closely resembles the natural substrate. Biochemical assays revealed that while PsChs1 binds and hydrolyzes UDP-GlcN, it fails to polymerize it. To uncover the structural basis of this stringent discrimination, we determined the cryo-EM structure of PsChs1 in complex with UDP-GlcN and GlcN. The structure, supported by molecular dynamics simulations, captures a non-productive state. In this state, a conserved arginine (Arg538) experiences electrostatic repulsion from the C2 ammonium group of UDP-GlcN. Concurrently, the loss of acetyl-dependent interactions displaces the 4'-OH group of the acceptor GlcN beyond the distance required for nucleophilic attack. Together, these findings reveal an acetyl-dependent proofreading mechanism that dictates the strict synthetic fidelity of chitin synthase.
format Artículo científico
id pubmed_41795371
institution PubMed
language en
publishDate 2026
publisher Biochemical and biophysical research communications
record_format pubmed
spellingShingle An acetyl-dependent proofreading mechanism governs synthetic fidelity in Phytophthora sojae chitin synthase.
Zhang, Xiaoyue
Li, Pengwei
Niu, Suhao
Gao, Kun
Sun, Mengsi
Wu, Yujie
Bi, Yunchen
Chitin Synthase
Phytophthora
Molecular Dynamics Simulation
Cryoelectron Microscopy
Chitin
Substrate Specificity
An acetyl-dependent proofreading mechanism governs synthetic fidelity in Phytophthora sojae chitin synthase. Zhang, Xiaoyue Li, Pengwei Niu, Suhao Gao, Kun Sun, Mengsi Wu, Yujie Bi, Yunchen Chitin Synthase Phytophthora Molecular Dynamics Simulation Cryoelectron Microscopy Chitin Substrate Specificity Chitin is an essential structural polysaccharide in fungal and oomycete cell walls, providing mechanical strength and integrity due to its crystalline, insoluble nature. Chitin synthase (CHS) is the key enzyme responsible for its biosynthesis, orchestrating the sequential steps of substrate binding, hydrolysis, glycosidic bond formation, and polymer translocation. However, the molecular mechanism that governs its synthetic fidelity remains unclear. To address this, we investigated whether Phytophthora sojae chitin synthase 1 (PsChs1) can utilize UDP-glucosamine (UDP-GlcN), a deacetylated substrate analog that closely resembles the natural substrate. Biochemical assays revealed that while PsChs1 binds and hydrolyzes UDP-GlcN, it fails to polymerize it. To uncover the structural basis of this stringent discrimination, we determined the cryo-EM structure of PsChs1 in complex with UDP-GlcN and GlcN. The structure, supported by molecular dynamics simulations, captures a non-productive state. In this state, a conserved arginine (Arg538) experiences electrostatic repulsion from the C2 ammonium group of UDP-GlcN. Concurrently, the loss of acetyl-dependent interactions displaces the 4'-OH group of the acceptor GlcN beyond the distance required for nucleophilic attack. Together, these findings reveal an acetyl-dependent proofreading mechanism that dictates the strict synthetic fidelity of chitin synthase.
title An acetyl-dependent proofreading mechanism governs synthetic fidelity in Phytophthora sojae chitin synthase.
topic Chitin Synthase
Phytophthora
Molecular Dynamics Simulation
Cryoelectron Microscopy
Chitin
Substrate Specificity
url https://pubmed.ncbi.nlm.nih.gov/41795371/