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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Advanced healthcare materials
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41797233/ |
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| _version_ | 1868266076978020354 |
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| author | Dani, Finn Duin, Sarah Akkineni, Ashwini Rahul Lehmann, Susann Ludwig, Barbara Kühl, Michael Gelinsky, Michael Lode, Anja |
| author_facet | Dani, Finn Duin, Sarah Akkineni, Ashwini Rahul Lehmann, Susann Ludwig, Barbara Kühl, Michael Gelinsky, Michael Lode, Anja Dani, Finn Duin, Sarah Akkineni, Ashwini Rahul Lehmann, Susann Ludwig, Barbara Kühl, Michael Gelinsky, Michael Lode, Anja |
| collection | PubMed - marine biology |
| contents | Oxygen Supply of Islets of Langerhans by Photosynthetically Active Microalgae in Bioprinted Co-Cultures Maintains Their Function in a Hypoxic Environment. Dani, Finn Duin, Sarah Akkineni, Ashwini Rahul Lehmann, Susann Ludwig, Barbara Kühl, Michael Gelinsky, Michael Lode, Anja Islets of Langerhans Coculture Techniques Oxygen Microalgae Photosynthesis Animals Bioprinting Hydrogels Scenedesmus Alginates Glucose Insulin Insulin Secretion Type 1 diabetes mellitus (T1D) is characterized by the autoimmune destruction of pancreatic beta cells, leading to insulin deficiency and necessitating lifelong external insulin administration. The transplantation of allogenic islets is a promising therapeutic approach, whereby their macro-encapsulation offers immune protection but restricts oxygenation after transplantation. This study addresses the challenge of oxygen supply by developing a spatially structured co-culture system using bioprinting, in which both pancreatic islets and the photosynthetically active microalga Scenedesmus sp. are embedded in alginate-based hydrogels. Key environmental parameters for long-term co-cultivation were developed and systematically optimized: red light illumination was identified as non-detrimental to islet viability and function while supporting microalgal photosynthesis at the same time, and a co-culture medium was formulated to fulfill the metabolic requirements of both cell types. In direct co-culture experiments under hypoxic conditions, microalgae generated sufficient oxygen to maintain normoxic conditions, thereby preserving islet viability and glucose-stimulated insulin secretion over several days. The results demonstrate that spatially organized bioprinting enables the close proximity of islets and microalgae, facilitating effective oxygen transfer in vitro. This work establishes a robust framework for functional mammalian-microalgae co-cultures, optimizing conditions to reliably maintain cell health and function through photosynthetically generated oxygen. |
| format | Artículo científico |
| id | pubmed_41797233 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Advanced healthcare materials |
| record_format | pubmed |
| spellingShingle | Oxygen Supply of Islets of Langerhans by Photosynthetically Active Microalgae in Bioprinted Co-Cultures Maintains Their Function in a Hypoxic Environment. Dani, Finn Duin, Sarah Akkineni, Ashwini Rahul Lehmann, Susann Ludwig, Barbara Kühl, Michael Gelinsky, Michael Lode, Anja Islets of Langerhans Coculture Techniques Oxygen Microalgae Photosynthesis Animals Bioprinting Hydrogels Scenedesmus Alginates Glucose Insulin Insulin Secretion Oxygen Supply of Islets of Langerhans by Photosynthetically Active Microalgae in Bioprinted Co-Cultures Maintains Their Function in a Hypoxic Environment. Dani, Finn Duin, Sarah Akkineni, Ashwini Rahul Lehmann, Susann Ludwig, Barbara Kühl, Michael Gelinsky, Michael Lode, Anja Islets of Langerhans Coculture Techniques Oxygen Microalgae Photosynthesis Animals Bioprinting Hydrogels Scenedesmus Alginates Glucose Insulin Insulin Secretion Type 1 diabetes mellitus (T1D) is characterized by the autoimmune destruction of pancreatic beta cells, leading to insulin deficiency and necessitating lifelong external insulin administration. The transplantation of allogenic islets is a promising therapeutic approach, whereby their macro-encapsulation offers immune protection but restricts oxygenation after transplantation. This study addresses the challenge of oxygen supply by developing a spatially structured co-culture system using bioprinting, in which both pancreatic islets and the photosynthetically active microalga Scenedesmus sp. are embedded in alginate-based hydrogels. Key environmental parameters for long-term co-cultivation were developed and systematically optimized: red light illumination was identified as non-detrimental to islet viability and function while supporting microalgal photosynthesis at the same time, and a co-culture medium was formulated to fulfill the metabolic requirements of both cell types. In direct co-culture experiments under hypoxic conditions, microalgae generated sufficient oxygen to maintain normoxic conditions, thereby preserving islet viability and glucose-stimulated insulin secretion over several days. The results demonstrate that spatially organized bioprinting enables the close proximity of islets and microalgae, facilitating effective oxygen transfer in vitro. This work establishes a robust framework for functional mammalian-microalgae co-cultures, optimizing conditions to reliably maintain cell health and function through photosynthetically generated oxygen. |
| title | Oxygen Supply of Islets of Langerhans by Photosynthetically Active Microalgae in Bioprinted Co-Cultures Maintains Their Function in a Hypoxic Environment. |
| topic | Islets of Langerhans Coculture Techniques Oxygen Microalgae Photosynthesis Animals Bioprinting Hydrogels Scenedesmus Alginates Glucose Insulin Insulin Secretion |
| url | https://pubmed.ncbi.nlm.nih.gov/41797233/ |