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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Journal of immunology (Baltimore, Md. : 1950)
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41802195/ |
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| _version_ | 1868266074601947137 |
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| author | Liu, Gao-Peng Li, Zhuo-Cong Li, Qi Zheng, Zheng-Gao Lin, Fei-Bi Zhang, Gui-Ying Zhang, Zhi-Min Li, Shun Li, Tao Zhao, Jin-Dong |
| author_facet | Liu, Gao-Peng Li, Zhuo-Cong Li, Qi Zheng, Zheng-Gao Lin, Fei-Bi Zhang, Gui-Ying Zhang, Zhi-Min Li, Shun Li, Tao Zhao, Jin-Dong Liu, Gao-Peng Li, Zhuo-Cong Li, Qi Zheng, Zheng-Gao Lin, Fei-Bi Zhang, Gui-Ying Zhang, Zhi-Min Li, Shun Li, Tao Zhao, Jin-Dong |
| collection | PubMed - marine biology |
| contents | Microalgal delivery of recombinant fish interferon modulates gut microbiota and enhances antiviral immunity in fish. Liu, Gao-Peng Li, Zhuo-Cong Li, Qi Zheng, Zheng-Gao Lin, Fei-Bi Zhang, Gui-Ying Zhang, Zhi-Min Li, Shun Li, Tao Zhao, Jin-Dong Animals Gastrointestinal Microbiome Zebrafish Fish Diseases Interferons Recombinant Proteins Microalgae Antiviral Agents Herpesviridae Infections Herpesviridae Carps Viral infections remain a challenge to aquaculture, resulting in severe economic losses and threatening fish health worldwide. As a key immunomodulatory and antiviral factor, interferon (IFN) plays a crucial role in regulating immune responses. We constructed a high-level expression strain of recombinant interferon (Rec-IFN) using Synechococcus sp. PCC 7002, which also served as a delivery system, and evaluated its efficacy as a dietary immunostimulant in gibel carp (Carassius gibelio) and zebrafish (Danio rerio). Analysis of the intestinal microbiome indicated that the Rec-IFN diet promoted beneficial bacteria such as Cetobacterium while reducing opportunistic pathogens including Aeromonas and Vibrio in gibel carp. Notably, after Cyprinid herpesvirus-2 (CyHV-2) infection, the Rec-IFN diet enhanced microbial connectivity, helping to preserve gut microbiota function. In zebrafish, the Rec-IFN diet increased species richness and evenness, while reducing opportunistic pathogens such as Vibrio. Transcriptomic analysis revealed specific activation of the Toll-like receptor signaling pathway and a reduction of immune overstimulation following infection in zebrafish. Our findings demonstrate that the Rec-IFN diet significantly enhanced the host IFN response and alleviated virus-induced damage to intestinal and immune organs, reduced viral load, and decreased mortality. This research offers a protective effect by reducing the severity of infections caused by both DNA virus (CyHV-2) and RNA virus (SVCV). It provides new insights into the application of Rec-IFN microalgae as an effective oral immunotherapeutic strategy for reducing losses from viral infections in aquaculture. |
| format | Artículo científico |
| id | pubmed_41802195 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Journal of immunology (Baltimore, Md. : 1950) |
| record_format | pubmed |
| spellingShingle | Microalgal delivery of recombinant fish interferon modulates gut microbiota and enhances antiviral immunity in fish. Liu, Gao-Peng Li, Zhuo-Cong Li, Qi Zheng, Zheng-Gao Lin, Fei-Bi Zhang, Gui-Ying Zhang, Zhi-Min Li, Shun Li, Tao Zhao, Jin-Dong Animals Gastrointestinal Microbiome Zebrafish Fish Diseases Interferons Recombinant Proteins Microalgae Antiviral Agents Herpesviridae Infections Herpesviridae Carps Microalgal delivery of recombinant fish interferon modulates gut microbiota and enhances antiviral immunity in fish. Liu, Gao-Peng Li, Zhuo-Cong Li, Qi Zheng, Zheng-Gao Lin, Fei-Bi Zhang, Gui-Ying Zhang, Zhi-Min Li, Shun Li, Tao Zhao, Jin-Dong Animals Gastrointestinal Microbiome Zebrafish Fish Diseases Interferons Recombinant Proteins Microalgae Antiviral Agents Herpesviridae Infections Herpesviridae Carps Viral infections remain a challenge to aquaculture, resulting in severe economic losses and threatening fish health worldwide. As a key immunomodulatory and antiviral factor, interferon (IFN) plays a crucial role in regulating immune responses. We constructed a high-level expression strain of recombinant interferon (Rec-IFN) using Synechococcus sp. PCC 7002, which also served as a delivery system, and evaluated its efficacy as a dietary immunostimulant in gibel carp (Carassius gibelio) and zebrafish (Danio rerio). Analysis of the intestinal microbiome indicated that the Rec-IFN diet promoted beneficial bacteria such as Cetobacterium while reducing opportunistic pathogens including Aeromonas and Vibrio in gibel carp. Notably, after Cyprinid herpesvirus-2 (CyHV-2) infection, the Rec-IFN diet enhanced microbial connectivity, helping to preserve gut microbiota function. In zebrafish, the Rec-IFN diet increased species richness and evenness, while reducing opportunistic pathogens such as Vibrio. Transcriptomic analysis revealed specific activation of the Toll-like receptor signaling pathway and a reduction of immune overstimulation following infection in zebrafish. Our findings demonstrate that the Rec-IFN diet significantly enhanced the host IFN response and alleviated virus-induced damage to intestinal and immune organs, reduced viral load, and decreased mortality. This research offers a protective effect by reducing the severity of infections caused by both DNA virus (CyHV-2) and RNA virus (SVCV). It provides new insights into the application of Rec-IFN microalgae as an effective oral immunotherapeutic strategy for reducing losses from viral infections in aquaculture. |
| title | Microalgal delivery of recombinant fish interferon modulates gut microbiota and enhances antiviral immunity in fish. |
| topic | Animals Gastrointestinal Microbiome Zebrafish Fish Diseases Interferons Recombinant Proteins Microalgae Antiviral Agents Herpesviridae Infections Herpesviridae Carps |
| url | https://pubmed.ncbi.nlm.nih.gov/41802195/ |