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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Journal of immunology (Baltimore, Md. : 1950)
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41802195/ |
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Table of Contents:
- Microalgal delivery of recombinant fish interferon modulates gut microbiota and enhances antiviral immunity in fish. Liu, Gao-Peng Li, Zhuo-Cong Li, Qi Zheng, Zheng-Gao Lin, Fei-Bi Zhang, Gui-Ying Zhang, Zhi-Min Li, Shun Li, Tao Zhao, Jin-Dong Animals Gastrointestinal Microbiome Zebrafish Fish Diseases Interferons Recombinant Proteins Microalgae Antiviral Agents Herpesviridae Infections Herpesviridae Carps Viral infections remain a challenge to aquaculture, resulting in severe economic losses and threatening fish health worldwide. As a key immunomodulatory and antiviral factor, interferon (IFN) plays a crucial role in regulating immune responses. We constructed a high-level expression strain of recombinant interferon (Rec-IFN) using Synechococcus sp. PCC 7002, which also served as a delivery system, and evaluated its efficacy as a dietary immunostimulant in gibel carp (Carassius gibelio) and zebrafish (Danio rerio). Analysis of the intestinal microbiome indicated that the Rec-IFN diet promoted beneficial bacteria such as Cetobacterium while reducing opportunistic pathogens including Aeromonas and Vibrio in gibel carp. Notably, after Cyprinid herpesvirus-2 (CyHV-2) infection, the Rec-IFN diet enhanced microbial connectivity, helping to preserve gut microbiota function. In zebrafish, the Rec-IFN diet increased species richness and evenness, while reducing opportunistic pathogens such as Vibrio. Transcriptomic analysis revealed specific activation of the Toll-like receptor signaling pathway and a reduction of immune overstimulation following infection in zebrafish. Our findings demonstrate that the Rec-IFN diet significantly enhanced the host IFN response and alleviated virus-induced damage to intestinal and immune organs, reduced viral load, and decreased mortality. This research offers a protective effect by reducing the severity of infections caused by both DNA virus (CyHV-2) and RNA virus (SVCV). It provides new insights into the application of Rec-IFN microalgae as an effective oral immunotherapeutic strategy for reducing losses from viral infections in aquaculture.