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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Parasites, hosts and diseases
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41806821/ |
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| _version_ | 1868266074594607105 |
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| author | Kim, Seung-Hyeon Lee, Hye-Mi Lee, Donghyun Kim, Hyoun Joong Bathige, S D N K Grijalva-Chon, José Manuel da Silva, Patricia Mirella Park, Kyung-Il |
| author_facet | Kim, Seung-Hyeon Lee, Hye-Mi Lee, Donghyun Kim, Hyoun Joong Bathige, S D N K Grijalva-Chon, José Manuel da Silva, Patricia Mirella Park, Kyung-Il Kim, Seung-Hyeon Lee, Hye-Mi Lee, Donghyun Kim, Hyoun Joong Bathige, S D N K Grijalva-Chon, José Manuel da Silva, Patricia Mirella Park, Kyung-Il |
| collection | PubMed - marine biology |
| contents | Quantitative PCR surveillance of Perkinsus marinus in Crassostrea virginica and Magallana gigas across the USA, Mexico, Brazil, and Korea. Kim, Seung-Hyeon Lee, Hye-Mi Lee, Donghyun Kim, Hyoun Joong Bathige, S D N K Grijalva-Chon, José Manuel da Silva, Patricia Mirella Park, Kyung-Il Animals Alveolata Aquaculture Real-Time Polymerase Chain Reaction Crassostrea Republic of Korea Mexico Brazil Ostreidae United States Prevalence Sensitivity and Specificity Perkinsus marinus is a significant pathogen in oyster aquaculture with expanding host and geographic ranges. This study evaluated the prevalence and infection intensity of P. marinus in major oyster farming regions across the USA, Mexico, Brazil, and Korea using a quantitative PCR (P. marinus-specific TaqMan quantitative PCR assay, Pm-qPCR) assay. Eastern oysters (Crassostrea virginica) were sampled from 7 USA sites, while Pacific oysters (Magallana gigas) were collected from Mexico, Brazil, and Korea. Compared to conventional PCR, the Pm-qPCR assay demonstrated significantly higher sensitivity, detecting P. marinus in >80.0% of samples at most sites and up to 100.0% in Port Norris, USA. Lower prevalence was found in Wellfleet, USA (58.0%) and Korean sites (63.0%-70.0%). The lowest infection intensities ( |
| format | Artículo científico |
| id | pubmed_41806821 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Parasites, hosts and diseases |
| record_format | pubmed |
| spellingShingle | Quantitative PCR surveillance of Perkinsus marinus in Crassostrea virginica and Magallana gigas across the USA, Mexico, Brazil, and Korea. Kim, Seung-Hyeon Lee, Hye-Mi Lee, Donghyun Kim, Hyoun Joong Bathige, S D N K Grijalva-Chon, José Manuel da Silva, Patricia Mirella Park, Kyung-Il Animals Alveolata Aquaculture Real-Time Polymerase Chain Reaction Crassostrea Republic of Korea Mexico Brazil Ostreidae United States Prevalence Sensitivity and Specificity Quantitative PCR surveillance of Perkinsus marinus in Crassostrea virginica and Magallana gigas across the USA, Mexico, Brazil, and Korea. Kim, Seung-Hyeon Lee, Hye-Mi Lee, Donghyun Kim, Hyoun Joong Bathige, S D N K Grijalva-Chon, José Manuel da Silva, Patricia Mirella Park, Kyung-Il Animals Alveolata Aquaculture Real-Time Polymerase Chain Reaction Crassostrea Republic of Korea Mexico Brazil Ostreidae United States Prevalence Sensitivity and Specificity Perkinsus marinus is a significant pathogen in oyster aquaculture with expanding host and geographic ranges. This study evaluated the prevalence and infection intensity of P. marinus in major oyster farming regions across the USA, Mexico, Brazil, and Korea using a quantitative PCR (P. marinus-specific TaqMan quantitative PCR assay, Pm-qPCR) assay. Eastern oysters (Crassostrea virginica) were sampled from 7 USA sites, while Pacific oysters (Magallana gigas) were collected from Mexico, Brazil, and Korea. Compared to conventional PCR, the Pm-qPCR assay demonstrated significantly higher sensitivity, detecting P. marinus in >80.0% of samples at most sites and up to 100.0% in Port Norris, USA. Lower prevalence was found in Wellfleet, USA (58.0%) and Korean sites (63.0%-70.0%). The lowest infection intensities ( |
| title | Quantitative PCR surveillance of Perkinsus marinus in Crassostrea virginica and Magallana gigas across the USA, Mexico, Brazil, and Korea. |
| topic | Animals Alveolata Aquaculture Real-Time Polymerase Chain Reaction Crassostrea Republic of Korea Mexico Brazil Ostreidae United States Prevalence Sensitivity and Specificity |
| url | https://pubmed.ncbi.nlm.nih.gov/41806821/ |