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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41825573/ |
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Table of Contents:
- First identification of nuclear factor kappa B inhibitor delta (NFKBID) in Platichthys stellatus: Molecular cloning and expression patterns. Ji, Chae-Yeong Kang, Gyoungsik Kim, Kyung-Ho Sohn, Min-Young Son, Ha-Jeong Jeong, Su Bhin Kim, In Gu Park, Chan-Il Animals Cloning, Molecular Phylogeny Fish Proteins Flounder Novirhabdovirus Fish Diseases Amino Acid Sequence Streptococcal Infections Gene Expression Regulation Streptococcus In this study, the nuclear factor kappa B inhibitor delta gene (NFKBID) of the starry flounder, Platichthys stellatus, was identified through next-generation sequencing (NGS) and verified by cloning and sequencing. The multiple sequence alignment and phylogenetic analysis revealed high conservation of NFKBID among several fish species. The tissue distribution of NFKBID in healthy P. stellatus was analyzed by quantitative PCR, revealing expression in almost all tissues, with relatively high levels in the gills, skin, and trunk kidney. Furthermore, significant changes in NFKBID expression were observed in starry flounder following infection with Streptococcus parauberis PH0710 and viral hemorrhagic septicemia virus (VHSV). Infection with S. parauberis PH0710 downregulated NFKBID in most tissues except the intestine. In contrast, VHSV infection showed a significant upregulation of expression in most tissues during the early phase. These results suggest that immune-related responses to bacterial and viral infections in starry flounder are associated with changes in NFKBID expression. This study provides fundamental molecular insights into the potential involvement of NFKBID in immune-related processes in teleost fish.