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| Main Authors: | , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Veterinary immunology and immunopathology
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41855770/ |
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| _version_ | 1868266071853629441 |
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| author | Guha, Ritam Pandey, Sudarshan Byadgi, Omkar Vijay Chen, Shih-Chu Elumalai, Preetham |
| author_facet | Guha, Ritam Pandey, Sudarshan Byadgi, Omkar Vijay Chen, Shih-Chu Elumalai, Preetham Guha, Ritam Pandey, Sudarshan Byadgi, Omkar Vijay Chen, Shih-Chu Elumalai, Preetham |
| collection | PubMed - marine biology |
| contents | Identification, molecular typing, antimicrobial susceptibility, and experimental pathogenicity of Streptococcus agalactiae isolated from Tilapia. Guha, Ritam Pandey, Sudarshan Byadgi, Omkar Vijay Chen, Shih-Chu Elumalai, Preetham Animals Streptococcus agalactiae Fish Diseases Streptococcal Infections Tilapia Virulence Microbial Sensitivity Tests Molecular Typing Cytokines Anti-Bacterial Agents India Streptococcosis caused by Streptococcus agalactiae is widespread in the world, especially in south Asia and southeast Asia. In this study, an S. agalactiae isolate collected from diseased fish in India was molecularly confirmed through GAPDH-targeted PCR, and further validated by sequencing. Genotyping revealed distinct genomic relationships among regional isolates; pulsed-field gel electrophoresis identified three pulsotypes with the Indian isolate clustering closely with two Taiwanese isolates under both ApaI and SmaI digestion, while ERIC-PCR grouped the Indian strain with a Taiwanese strain at > 92% similarity. Antibiotic susceptibility test showed that the Indian isolate was resistant to tetracycline, in contrast to the control strain. Pathogenicity assays determined an LD of 1.7 × 10⁵ CFU/ml, and infected fish displayed typical clinical signs consistent with streptococcosis. Pro-inflammatory cytokines (IL-1β, IL-8, IFN-γ) were significantly upregulated in head kidney and spleen tissues at 48 h post infection, although IL-8 and IL-1β showed earlier expression in the spleen. These results characterize the molecular identity, virulence, and immunopathological profile of an Indian S. agalactiae strain, providing critical insights for disease surveillance in tilapia aquaculture. |
| format | Artículo científico |
| id | pubmed_41855770 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Veterinary immunology and immunopathology |
| record_format | pubmed |
| spellingShingle | Identification, molecular typing, antimicrobial susceptibility, and experimental pathogenicity of Streptococcus agalactiae isolated from Tilapia. Guha, Ritam Pandey, Sudarshan Byadgi, Omkar Vijay Chen, Shih-Chu Elumalai, Preetham Animals Streptococcus agalactiae Fish Diseases Streptococcal Infections Tilapia Virulence Microbial Sensitivity Tests Molecular Typing Cytokines Anti-Bacterial Agents India Identification, molecular typing, antimicrobial susceptibility, and experimental pathogenicity of Streptococcus agalactiae isolated from Tilapia. Guha, Ritam Pandey, Sudarshan Byadgi, Omkar Vijay Chen, Shih-Chu Elumalai, Preetham Animals Streptococcus agalactiae Fish Diseases Streptococcal Infections Tilapia Virulence Microbial Sensitivity Tests Molecular Typing Cytokines Anti-Bacterial Agents India Streptococcosis caused by Streptococcus agalactiae is widespread in the world, especially in south Asia and southeast Asia. In this study, an S. agalactiae isolate collected from diseased fish in India was molecularly confirmed through GAPDH-targeted PCR, and further validated by sequencing. Genotyping revealed distinct genomic relationships among regional isolates; pulsed-field gel electrophoresis identified three pulsotypes with the Indian isolate clustering closely with two Taiwanese isolates under both ApaI and SmaI digestion, while ERIC-PCR grouped the Indian strain with a Taiwanese strain at > 92% similarity. Antibiotic susceptibility test showed that the Indian isolate was resistant to tetracycline, in contrast to the control strain. Pathogenicity assays determined an LD of 1.7 × 10⁵ CFU/ml, and infected fish displayed typical clinical signs consistent with streptococcosis. Pro-inflammatory cytokines (IL-1β, IL-8, IFN-γ) were significantly upregulated in head kidney and spleen tissues at 48 h post infection, although IL-8 and IL-1β showed earlier expression in the spleen. These results characterize the molecular identity, virulence, and immunopathological profile of an Indian S. agalactiae strain, providing critical insights for disease surveillance in tilapia aquaculture. |
| title | Identification, molecular typing, antimicrobial susceptibility, and experimental pathogenicity of Streptococcus agalactiae isolated from Tilapia. |
| topic | Animals Streptococcus agalactiae Fish Diseases Streptococcal Infections Tilapia Virulence Microbial Sensitivity Tests Molecular Typing Cytokines Anti-Bacterial Agents India |
| url | https://pubmed.ncbi.nlm.nih.gov/41855770/ |