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| Main Authors: | , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Marine environmental research
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41855937/ |
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| _version_ | 1868266071852580865 |
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| author | Jiang, Lijia Liang, Zhengjiao Williams, Tom A Deng, Yinan Yu, Hao Hao, Qian Cao, Jun Zhou, Hanghai Lai, Hongfei Chen, Jiawang Chen, Haixin Zhang, Chunfang |
| author_facet | Jiang, Lijia Liang, Zhengjiao Williams, Tom A Deng, Yinan Yu, Hao Hao, Qian Cao, Jun Zhou, Hanghai Lai, Hongfei Chen, Jiawang Chen, Haixin Zhang, Chunfang Jiang, Lijia Liang, Zhengjiao Williams, Tom A Deng, Yinan Yu, Hao Hao, Qian Cao, Jun Zhou, Hanghai Lai, Hongfei Chen, Jiawang Chen, Haixin Zhang, Chunfang |
| collection | PubMed - marine biology |
| contents | Vertical stratification and metabolic versatility of methanogens in Haima cold seep sediments: Alkane-fueled acetoclastic methanogenesis revealed by metagenomics and experimental verification. Jiang, Lijia Liang, Zhengjiao Williams, Tom A Deng, Yinan Yu, Hao Hao, Qian Cao, Jun Zhou, Hanghai Lai, Hongfei Chen, Jiawang Chen, Haixin Zhang, Chunfang Geologic Sediments Alkanes Metagenomics Methane Archaea Oxidoreductases Cold seeps are hot spots for studying the biogeochemical processes mediated by methyl-coenzyme M reductase (MCR) containing archaea, yet these processes remain poorly understood. Here, we investigated the microbial communities in a 5-m-long sediment core encompassing sulfate-methane transition zone (SMTZ), collected from the Haima cold seep. We focused on distinct biogeochemical characteristics and metagenomics to study the vertical patterns of mcrA-containing archaea and their role in alkane metabolism. Background alkane analysis showed that long chain alkanes were dominant in the sediment core. Metagenomic and quantitative PCR (qPCR) analysis revealed that the abundance of mcrA gene within and below the SMTZ were substantially higher than those above the SMTZ. We recovered 21 mcrA-containing MAGs, 11 affiliated with Methanosarcinaceae. One MAG (CG1-BIN56) encoded complete key genes for all three methanogenic pathways. Functional gene profiles suggested that acetoclastic methanogenesis is the dominant pathway. Furthermore, a 10-month anaerobic enrichment with n-alkanes (C/C) using SMTZ (CG15: 350 cmbsf) inocula exhibited high degradation rate (C: 94.2% ± 2.5%, C: 66.8% ± 2.8%) accompanied by acetate accumulation. These findings suggest that acetate, as a likely intermediate associated with alkane degradation process, potentially fuels acetoclastic methanogenesis. This indicates a likely syntrophic interaction between alkane-degrading and methane-cycling microorganisms in cold seep sediments. |
| format | Artículo científico |
| id | pubmed_41855937 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Marine environmental research |
| record_format | pubmed |
| spellingShingle | Vertical stratification and metabolic versatility of methanogens in Haima cold seep sediments: Alkane-fueled acetoclastic methanogenesis revealed by metagenomics and experimental verification. Jiang, Lijia Liang, Zhengjiao Williams, Tom A Deng, Yinan Yu, Hao Hao, Qian Cao, Jun Zhou, Hanghai Lai, Hongfei Chen, Jiawang Chen, Haixin Zhang, Chunfang Geologic Sediments Alkanes Metagenomics Methane Archaea Oxidoreductases Vertical stratification and metabolic versatility of methanogens in Haima cold seep sediments: Alkane-fueled acetoclastic methanogenesis revealed by metagenomics and experimental verification. Jiang, Lijia Liang, Zhengjiao Williams, Tom A Deng, Yinan Yu, Hao Hao, Qian Cao, Jun Zhou, Hanghai Lai, Hongfei Chen, Jiawang Chen, Haixin Zhang, Chunfang Geologic Sediments Alkanes Metagenomics Methane Archaea Oxidoreductases Cold seeps are hot spots for studying the biogeochemical processes mediated by methyl-coenzyme M reductase (MCR) containing archaea, yet these processes remain poorly understood. Here, we investigated the microbial communities in a 5-m-long sediment core encompassing sulfate-methane transition zone (SMTZ), collected from the Haima cold seep. We focused on distinct biogeochemical characteristics and metagenomics to study the vertical patterns of mcrA-containing archaea and their role in alkane metabolism. Background alkane analysis showed that long chain alkanes were dominant in the sediment core. Metagenomic and quantitative PCR (qPCR) analysis revealed that the abundance of mcrA gene within and below the SMTZ were substantially higher than those above the SMTZ. We recovered 21 mcrA-containing MAGs, 11 affiliated with Methanosarcinaceae. One MAG (CG1-BIN56) encoded complete key genes for all three methanogenic pathways. Functional gene profiles suggested that acetoclastic methanogenesis is the dominant pathway. Furthermore, a 10-month anaerobic enrichment with n-alkanes (C/C) using SMTZ (CG15: 350 cmbsf) inocula exhibited high degradation rate (C: 94.2% ± 2.5%, C: 66.8% ± 2.8%) accompanied by acetate accumulation. These findings suggest that acetate, as a likely intermediate associated with alkane degradation process, potentially fuels acetoclastic methanogenesis. This indicates a likely syntrophic interaction between alkane-degrading and methane-cycling microorganisms in cold seep sediments. |
| title | Vertical stratification and metabolic versatility of methanogens in Haima cold seep sediments: Alkane-fueled acetoclastic methanogenesis revealed by metagenomics and experimental verification. |
| topic | Geologic Sediments Alkanes Metagenomics Methane Archaea Oxidoreductases |
| url | https://pubmed.ncbi.nlm.nih.gov/41855937/ |