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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Fish & shellfish immunology
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41864287/ |
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| _version_ | 1868266069684125697 |
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| author | Ding, Xin Chen, Yuan Wang, Qingyue Yuan, Zihao Zhang, Jian Li, Xuepeng Xu, Hang Sun, Li |
| author_facet | Ding, Xin Chen, Yuan Wang, Qingyue Yuan, Zihao Zhang, Jian Li, Xuepeng Xu, Hang Sun, Li Ding, Xin Chen, Yuan Wang, Qingyue Yuan, Zihao Zhang, Jian Li, Xuepeng Xu, Hang Sun, Li |
| collection | PubMed - marine biology |
| contents | Spotted seabass Lateolabrax maculatus GSDMEa: a pyroptosis executor activated by CASP3/7 during bacterial infection. Ding, Xin Chen, Yuan Wang, Qingyue Yuan, Zihao Zhang, Jian Li, Xuepeng Xu, Hang Sun, Li Animals Fish Diseases Pyroptosis Bass Fish Proteins Immunity, Innate Photobacterium Gram-Negative Bacterial Infections Phylogeny Caspases Sequence Alignment Amino Acid Sequence Gene Expression Regulation Gene Expression Profiling Pyroptosis is a lytic form of programmed cell death executed by gasdermin, which is activated by caspase (CASP) cleavage. Pyroptosis plays a pivotal role in host defense against bacterial infection. Spotted seabass Lateolabrax maculatus, one of the major aquaculture fish in China, is vulnerable to the bacterial pathogen Photobacterium damselae subsp. piscicida (PDP), which causes significant economic losses due to high mortality outbreaks. To date, no study on L. maculatus gasdermin has been documented, and the role of gasdermin in bacterial infection is unclear. In this study, we identified from L. maculatus two gasdermin E (GSDME) (designated LmGSDMEa and LmGSDMEb, respectively) and three CASPs (designated LmCASP3, 6, and 7, respectively), and characterized their biological activities. LmCASP3 and LmCASP7, but not LmCASP6, directly induced apoptosis. LmCASP3/7 specifically cleaved LmGSDMEa at the NTVD site, generating an active N-terminal fragment (NT265) that converted apoptosis into pyroptosis. Unlike LmGSDMEa, LmGSDMEb could not trigger pyroptosis. When spotted seabass were challenged with PDP, the expression levels of LmCASP3/7, LmGSDMEa, and pyroptosis-associated inflammatory cytokines were upregulated in fish tissues. Consistently, PDP infection promoted the production and activation of LmGSDMEa and LmCASP3/7 in spotted seabass macrophages, resulting in pyroptotic cell death. Collectively, this study revealed the activation and regulation mechanism of GSDMEa-mediated pyroptosis in spotted seabass, and provided new insights into the role of pyroptosis in fish immunity during bacterial infection. |
| format | Artículo científico |
| id | pubmed_41864287 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | Spotted seabass Lateolabrax maculatus GSDMEa: a pyroptosis executor activated by CASP3/7 during bacterial infection. Ding, Xin Chen, Yuan Wang, Qingyue Yuan, Zihao Zhang, Jian Li, Xuepeng Xu, Hang Sun, Li Animals Fish Diseases Pyroptosis Bass Fish Proteins Immunity, Innate Photobacterium Gram-Negative Bacterial Infections Phylogeny Caspases Sequence Alignment Amino Acid Sequence Gene Expression Regulation Gene Expression Profiling Spotted seabass Lateolabrax maculatus GSDMEa: a pyroptosis executor activated by CASP3/7 during bacterial infection. Ding, Xin Chen, Yuan Wang, Qingyue Yuan, Zihao Zhang, Jian Li, Xuepeng Xu, Hang Sun, Li Animals Fish Diseases Pyroptosis Bass Fish Proteins Immunity, Innate Photobacterium Gram-Negative Bacterial Infections Phylogeny Caspases Sequence Alignment Amino Acid Sequence Gene Expression Regulation Gene Expression Profiling Pyroptosis is a lytic form of programmed cell death executed by gasdermin, which is activated by caspase (CASP) cleavage. Pyroptosis plays a pivotal role in host defense against bacterial infection. Spotted seabass Lateolabrax maculatus, one of the major aquaculture fish in China, is vulnerable to the bacterial pathogen Photobacterium damselae subsp. piscicida (PDP), which causes significant economic losses due to high mortality outbreaks. To date, no study on L. maculatus gasdermin has been documented, and the role of gasdermin in bacterial infection is unclear. In this study, we identified from L. maculatus two gasdermin E (GSDME) (designated LmGSDMEa and LmGSDMEb, respectively) and three CASPs (designated LmCASP3, 6, and 7, respectively), and characterized their biological activities. LmCASP3 and LmCASP7, but not LmCASP6, directly induced apoptosis. LmCASP3/7 specifically cleaved LmGSDMEa at the NTVD site, generating an active N-terminal fragment (NT265) that converted apoptosis into pyroptosis. Unlike LmGSDMEa, LmGSDMEb could not trigger pyroptosis. When spotted seabass were challenged with PDP, the expression levels of LmCASP3/7, LmGSDMEa, and pyroptosis-associated inflammatory cytokines were upregulated in fish tissues. Consistently, PDP infection promoted the production and activation of LmGSDMEa and LmCASP3/7 in spotted seabass macrophages, resulting in pyroptotic cell death. Collectively, this study revealed the activation and regulation mechanism of GSDMEa-mediated pyroptosis in spotted seabass, and provided new insights into the role of pyroptosis in fish immunity during bacterial infection. |
| title | Spotted seabass Lateolabrax maculatus GSDMEa: a pyroptosis executor activated by CASP3/7 during bacterial infection. |
| topic | Animals Fish Diseases Pyroptosis Bass Fish Proteins Immunity, Innate Photobacterium Gram-Negative Bacterial Infections Phylogeny Caspases Sequence Alignment Amino Acid Sequence Gene Expression Regulation Gene Expression Profiling |
| url | https://pubmed.ncbi.nlm.nih.gov/41864287/ |