The C-type lysozyme of Miichthys miiuy exhibits specific antibacterial activity against Gram-negative bacteria.
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| Sprache: | en |
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Fish & shellfish immunology
2026
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| author | Pan, Ziyue Zhao, Yan Shan, Siqi Xu, Tianjun Sun, Yuena |
| author_facet | Pan, Ziyue Zhao, Yan Shan, Siqi Xu, Tianjun Sun, Yuena Pan, Ziyue Zhao, Yan Shan, Siqi Xu, Tianjun Sun, Yuena |
| collection | PubMed - marine biology |
| contents | The C-type lysozyme of Miichthys miiuy exhibits specific antibacterial activity against Gram-negative bacteria. Pan, Ziyue Zhao, Yan Shan, Siqi Xu, Tianjun Sun, Yuena Animals Muramidase Immunity, Innate Gram-Negative Bacteria Amino Acid Sequence Fish Proteins Fish Diseases Sequence Alignment Phylogeny Base Sequence Gene Expression Profiling Anti-Bacterial Agents Perciformes Gene Expression Regulation Gram-Negative Bacterial Infections Lysozyme is a key antimicrobial molecule in the innate immune system of fish, playing a central role in defending against pathogen invasion. In this study, a novel c-type lysozyme (mmi-LyzC) was identified and functionally characterized from Miichthys miiuy. The full-length cDNA was 432 bp, encoding a 143-amino-acid protein containing a conserved LYZ_C domain, in which the active sites Glu and Asp were highly conserved across teleosts. After LPS challenge, the expression of mmi-LyzC in immune-related tissues was significantly upregulated, peaking at 48 h. The recombinant mmi-LyzC protein (rMmiLyzC) exhibited unexpectedly strong antimicrobial activity against two Gram-negative bacteria, while only moderate effect against a Gram-positive bacteria. The active protein was optimally produced by induction with 0.1 mM IPTG at 30 °C for 12 h, and it remained stable at pH 5-8. This study reveals the potent activity of mmi-LyzC against two Gram-negative bacteria and provides a potential target for improving bacterial disease resistance in aquaculture. |
| format | Artículo científico |
| id | pubmed_41866098 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | The C-type lysozyme of Miichthys miiuy exhibits specific antibacterial activity against Gram-negative bacteria. Pan, Ziyue Zhao, Yan Shan, Siqi Xu, Tianjun Sun, Yuena Animals Muramidase Immunity, Innate Gram-Negative Bacteria Amino Acid Sequence Fish Proteins Fish Diseases Sequence Alignment Phylogeny Base Sequence Gene Expression Profiling Anti-Bacterial Agents Perciformes Gene Expression Regulation Gram-Negative Bacterial Infections The C-type lysozyme of Miichthys miiuy exhibits specific antibacterial activity against Gram-negative bacteria. Pan, Ziyue Zhao, Yan Shan, Siqi Xu, Tianjun Sun, Yuena Animals Muramidase Immunity, Innate Gram-Negative Bacteria Amino Acid Sequence Fish Proteins Fish Diseases Sequence Alignment Phylogeny Base Sequence Gene Expression Profiling Anti-Bacterial Agents Perciformes Gene Expression Regulation Gram-Negative Bacterial Infections Lysozyme is a key antimicrobial molecule in the innate immune system of fish, playing a central role in defending against pathogen invasion. In this study, a novel c-type lysozyme (mmi-LyzC) was identified and functionally characterized from Miichthys miiuy. The full-length cDNA was 432 bp, encoding a 143-amino-acid protein containing a conserved LYZ_C domain, in which the active sites Glu and Asp were highly conserved across teleosts. After LPS challenge, the expression of mmi-LyzC in immune-related tissues was significantly upregulated, peaking at 48 h. The recombinant mmi-LyzC protein (rMmiLyzC) exhibited unexpectedly strong antimicrobial activity against two Gram-negative bacteria, while only moderate effect against a Gram-positive bacteria. The active protein was optimally produced by induction with 0.1 mM IPTG at 30 °C for 12 h, and it remained stable at pH 5-8. This study reveals the potent activity of mmi-LyzC against two Gram-negative bacteria and provides a potential target for improving bacterial disease resistance in aquaculture. |
| title | The C-type lysozyme of Miichthys miiuy exhibits specific antibacterial activity against Gram-negative bacteria. |
| topic | Animals Muramidase Immunity, Innate Gram-Negative Bacteria Amino Acid Sequence Fish Proteins Fish Diseases Sequence Alignment Phylogeny Base Sequence Gene Expression Profiling Anti-Bacterial Agents Perciformes Gene Expression Regulation Gram-Negative Bacterial Infections |
| url | https://pubmed.ncbi.nlm.nih.gov/41866098/ |