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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Science China. Life sciences
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41866455/ |
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Table of Contents:
- Ultra-fast generation of all-female grass carp via transplantation of female germline stem cells into zebrafish. Ye, Ding Hao, Yongkang Zhu, Junwen Wang, Chaofan Li, Linglu Li, Yongming Tu, Yi-Xuan Ren, Zhiqin Wang, Houpeng Chen, Zhengfang He, Mudan Wang, Xiaosi Sun, Yonghua Surrogate reproduction offers a promising biotechnological approach for accelerating aquaculture breeding. Grass carp (Ctenopharyngodon idellus), a key freshwater species, faces significant constraints in breeding due to its prolonged sexual maturation of 5 years and huge body size. Here, we establish an ultra-fast breeding strategy to generate all-female grass carp within six months via surrogate production in tiny laboratory fish, zebrafish (Danio rerio). We identify and isolate female GSCs from juvenile ovaries of 3-month-old grass carp. Three months after transplantation into zebrafish larvae, the donor-derived female GSCs undergo ultra-fast spermatogenesis and differentiate into functional grass carp sperm carrying X chromosomes. Fertilization of wild-type grass carp eggs with this sperm yields all-female offspring. This work demonstrates that fish female GSCs with XX chromosomes can differentiate into functional sperm in a short time in zebrafish gonadal somatic niche, opening a new avenue for precision breeding and sex control in aquaculture species.