Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Journal of fish biology
2026
|
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41879661/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266069654765569 |
|---|---|
| author | Xue, Zhuang Yan, Yuqing Qiu, Chenghao Chang, Juan Wang, Wei Pu, Hongyu |
| author_facet | Xue, Zhuang Yan, Yuqing Qiu, Chenghao Chang, Juan Wang, Wei Pu, Hongyu Xue, Zhuang Yan, Yuqing Qiu, Chenghao Chang, Juan Wang, Wei Pu, Hongyu |
| collection | PubMed - marine biology |
| contents | Calcium and magnesium ions are the main intrinsic factors that induce the activation of stickiness in the eggs of the fat greenling (Hexagrammos otakii). Xue, Zhuang Yan, Yuqing Qiu, Chenghao Chang, Juan Wang, Wei Pu, Hongyu In fish artificial breeding, adhesive eggs present a significant challenge for fertilization and reduce hatching success rates. Understanding the specific factors and mechanisms that influence egg adhesiveness, as well as developing effective de-adhesion methods, is crucial for improving fish reproduction efficiency. Fat greenling (Hexagrammos otakii), a typical marine teleost species that produces adhesive eggs, is widely distributed in the northwest Pacific Ocean and is considered a promising candidate for aquaculture. In this study, H. otakii eggs were used as a model to qualitatively analyse the effects of different pH levels (6.0, 7.0, 8.0 and 9.0), osmotic pressures (100, 400 and 1000 mOsm kg) and metal ions (Na, K, Ca, Mg, Mn and Cu) on egg adhesiveness. In addition, scanning electron microscopy was employed to examine the ultrastructural changes on the egg surface under different conditions. The results indicated that Ca and Mg are the primary factors in seawater that induce egg adhesiveness in H. otakii, while changes in pH and osmotic pressure neither triggered adhesiveness nor altered the underlying adhesive mechanism. Furthermore, the study evaluated the effectiveness of protease treatment for chemical de-adhesion. The results showed that treating the eggs with a 10 mg/L protease solution for 2 min successfully removed their adhesiveness, confirming the feasibility of chemical de-adhesion. This research provides significant theoretical insights for optimizing the artificial breeding techniques of H. otakii. |
| format | Artículo científico |
| id | pubmed_41879661 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Journal of fish biology |
| record_format | pubmed |
| spellingShingle | Calcium and magnesium ions are the main intrinsic factors that induce the activation of stickiness in the eggs of the fat greenling (Hexagrammos otakii). Xue, Zhuang Yan, Yuqing Qiu, Chenghao Chang, Juan Wang, Wei Pu, Hongyu Calcium and magnesium ions are the main intrinsic factors that induce the activation of stickiness in the eggs of the fat greenling (Hexagrammos otakii). Xue, Zhuang Yan, Yuqing Qiu, Chenghao Chang, Juan Wang, Wei Pu, Hongyu In fish artificial breeding, adhesive eggs present a significant challenge for fertilization and reduce hatching success rates. Understanding the specific factors and mechanisms that influence egg adhesiveness, as well as developing effective de-adhesion methods, is crucial for improving fish reproduction efficiency. Fat greenling (Hexagrammos otakii), a typical marine teleost species that produces adhesive eggs, is widely distributed in the northwest Pacific Ocean and is considered a promising candidate for aquaculture. In this study, H. otakii eggs were used as a model to qualitatively analyse the effects of different pH levels (6.0, 7.0, 8.0 and 9.0), osmotic pressures (100, 400 and 1000 mOsm kg) and metal ions (Na, K, Ca, Mg, Mn and Cu) on egg adhesiveness. In addition, scanning electron microscopy was employed to examine the ultrastructural changes on the egg surface under different conditions. The results indicated that Ca and Mg are the primary factors in seawater that induce egg adhesiveness in H. otakii, while changes in pH and osmotic pressure neither triggered adhesiveness nor altered the underlying adhesive mechanism. Furthermore, the study evaluated the effectiveness of protease treatment for chemical de-adhesion. The results showed that treating the eggs with a 10 mg/L protease solution for 2 min successfully removed their adhesiveness, confirming the feasibility of chemical de-adhesion. This research provides significant theoretical insights for optimizing the artificial breeding techniques of H. otakii. |
| title | Calcium and magnesium ions are the main intrinsic factors that induce the activation of stickiness in the eggs of the fat greenling (Hexagrammos otakii). |
| url | https://pubmed.ncbi.nlm.nih.gov/41879661/ |