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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Current research in parasitology & vector-borne diseases
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41890582/ |
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| _version_ | 1868266069637988354 |
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| author | Juhásová, Ľudmila Králová-Hromadová, Ivica Oboňa, Jozef Radačovská, Alžbeta Pintilioaie, Alexandru-Mihai Hromada, Martin Čisovská Bazsalovicsová, Eva |
| author_facet | Juhásová, Ľudmila Králová-Hromadová, Ivica Oboňa, Jozef Radačovská, Alžbeta Pintilioaie, Alexandru-Mihai Hromada, Martin Čisovská Bazsalovicsová, Eva Juhásová, Ľudmila Králová-Hromadová, Ivica Oboňa, Jozef Radačovská, Alžbeta Pintilioaie, Alexandru-Mihai Hromada, Martin Čisovská Bazsalovicsová, Eva |
| collection | PubMed - marine biology |
| contents | Design of species-specific molecular markers for PCR-based differentiation of bird louse flies , and (Diptera: Hippoboscidae). Juhásová, Ľudmila Králová-Hromadová, Ivica Oboňa, Jozef Radačovská, Alžbeta Pintilioaie, Alexandru-Mihai Hromada, Martin Čisovská Bazsalovicsová, Eva Louse flies of the genus (Hippoboscidae) are widespread avian ectoparasites of ecological and epidemiological importance, acting as blood-feeding parasites and potential vectors of avian and zoonotic pathogens. Traditional taxonomic identification can be challenging due to overlapping phenotypic traits, broad host associations, and the frequent occurrence of damaged specimens. This study aimed to develop a reliable, species-specific PCR-based molecular tool for differentiating three European louse fly species: , and . Mitochondrial cytochrome oxidase subunit 1 gene (1) sequences from different geographical populations of the studied species, both newly sequenced and retrieved from GenBank, were analysed to assess intraspecific variation and interspecific divergence. The 1 regions characterised by high interspecific polymorphism and no intraspecific variability were selected for the design and validation of nine species-specific primer sets. Four of these produced PCR products exclusively for their target species, demonstrating their effectiveness for species identification. This new single-step PCR-based approach for accurate and cost-effective molecular identification can efficiently supplement taxonomy methods based solely on morphology. The findings highlight the necessity of combined approaches integrating morphological and DNA-based methods to resolve the taxonomy of louse flies and underscore the importance of developing novel molecular tools to improve species delimitation. |
| format | Artículo científico |
| id | pubmed_41890582 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Current research in parasitology & vector-borne diseases |
| record_format | pubmed |
| spellingShingle | Design of species-specific molecular markers for PCR-based differentiation of bird louse flies , and (Diptera: Hippoboscidae). Juhásová, Ľudmila Králová-Hromadová, Ivica Oboňa, Jozef Radačovská, Alžbeta Pintilioaie, Alexandru-Mihai Hromada, Martin Čisovská Bazsalovicsová, Eva Design of species-specific molecular markers for PCR-based differentiation of bird louse flies , and (Diptera: Hippoboscidae). Juhásová, Ľudmila Králová-Hromadová, Ivica Oboňa, Jozef Radačovská, Alžbeta Pintilioaie, Alexandru-Mihai Hromada, Martin Čisovská Bazsalovicsová, Eva Louse flies of the genus (Hippoboscidae) are widespread avian ectoparasites of ecological and epidemiological importance, acting as blood-feeding parasites and potential vectors of avian and zoonotic pathogens. Traditional taxonomic identification can be challenging due to overlapping phenotypic traits, broad host associations, and the frequent occurrence of damaged specimens. This study aimed to develop a reliable, species-specific PCR-based molecular tool for differentiating three European louse fly species: , and . Mitochondrial cytochrome oxidase subunit 1 gene (1) sequences from different geographical populations of the studied species, both newly sequenced and retrieved from GenBank, were analysed to assess intraspecific variation and interspecific divergence. The 1 regions characterised by high interspecific polymorphism and no intraspecific variability were selected for the design and validation of nine species-specific primer sets. Four of these produced PCR products exclusively for their target species, demonstrating their effectiveness for species identification. This new single-step PCR-based approach for accurate and cost-effective molecular identification can efficiently supplement taxonomy methods based solely on morphology. The findings highlight the necessity of combined approaches integrating morphological and DNA-based methods to resolve the taxonomy of louse flies and underscore the importance of developing novel molecular tools to improve species delimitation. |
| title | Design of species-specific molecular markers for PCR-based differentiation of bird louse flies , and (Diptera: Hippoboscidae). |
| url | https://pubmed.ncbi.nlm.nih.gov/41890582/ |