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| Hauptverfasser: | , , , , , , , , |
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| Format: | Artículo científico |
| Sprache: | en |
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International journal of biological macromolecules
2026
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| Schlagworte: | |
| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/41905690/ |
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Inhaltsangabe:
- Conductive and antioxidant biopolymeric hydrogel-based bioinks with nanohybrids of protein amyloid nanofibrils‑gold nanoparticles for cardiac 3D bioprinting. Carvalho, João P F Teixeira, Maria C Luís, Jorge L Tavares, Bernardo L Oliveira, Helena Oliveira, José M Silvestre, Armando J D Vilela, Carla Freire, Carmen S R Antioxidants Hydrogels Ink Bioprinting Metal Nanoparticles Amyloid Gold Heart Myocytes, Cardiac Animals Rats Cell Line Tissue Engineering 3D bioprinting is a versatile technique that combines living cells and biomaterials to fabricate in vitro 3D living structures that resemble native tissues. These 3D platforms, with a higher complexity than traditional 2D cell cultures, emerge as new alternatives for biomedical research and tissue engineering. In this work, high performance conductive bioinks were developed by the combination of a hydrogel matrix of gellan gum (1.5 wt%) and carboxymethyl cellulose (1.0 wt%) with hybrid nanostructures composed of lysozyme amyloid nanofibrils and gold nanoparticles (LNF:AuNPs). The ink formulations show proper rheological properties, with a shear-thinning behavior and good elastic modulus (G') recovery rate (>85%), notorious printability, and enhanced conductivity (~3.0 mS cm) granted by the presence of LNF:AuNPs, viz. Ink_LNF:AuNPs_5% and Ink_LNF:AuNPs_15%. Moreover, the resulting hydrogels mimic the stiffness of myocardium (70%), and the incorporation of LNF:AuNPs reduces their degradation rate (from above 30% to 13.8 ± 2.4%) and improves their antioxidant activity (from 22.0 ± 0.8% to 75.1 ± 0.3%). The successful 3D bioprinting of bioinks laden with cardiomyocytes (H9c2 cells) originates complex structures that maintain high cell viability (90%) for 7 days, confirming their biocompatibility and potential for the 3D bioprinting of living structures that could be used as cardiac tissue platforms for different purposes.