Temperature modulates gut microbiome disruption and resistome enrichment in oxytetracycline-treated channel catfish ().
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| Auteurs principaux: | , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
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Microbiology spectrum
2026
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| _version_ | 1868266067032276992 |
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| author | Li, Xiran Wang, Hongye Abdelrahman, Hisham A Kelly, Anita M Roy, Luke A Soto, Esteban Wang, Luxin |
| author_facet | Li, Xiran Wang, Hongye Abdelrahman, Hisham A Kelly, Anita M Roy, Luke A Soto, Esteban Wang, Luxin Li, Xiran Wang, Hongye Abdelrahman, Hisham A Kelly, Anita M Roy, Luke A Soto, Esteban Wang, Luxin |
| collection | PubMed - marine biology |
| contents | Temperature modulates gut microbiome disruption and resistome enrichment in oxytetracycline-treated channel catfish (). Li, Xiran Wang, Hongye Abdelrahman, Hisham A Kelly, Anita M Roy, Luke A Soto, Esteban Wang, Luxin Oxytetracycline (OTC) is one of the few antibiotics approved by the U.S. Food and Drug Administration for catfish aquaculture. Unfortunately, OTC resistance has been frequently detected in production environments, with the fish gut identified as a potential hotspot for resistance selection. In aquaculture systems, water temperature is a critical factor influencing fish physiology, antibiotic pharmacokinetics, and water resistome development. However, its role in modulating OTC effects on the fish gut microbiome remains underexplored. This study examined temperature-dependent microbiome and resistome responses in channel catfish () when treated with OTC at 20°C, 25°C, and 30°C. Gut contents collected at treatment completion and after withdrawal were analyzed via metagenomic sequencing. In untreated fish, temperature alone shaped microbial structure and function, with the Shannon diversity increasing with temperatures and the β-diversity differing significantly across temperature groups. After OTC exposure, microbial responses were markedly temperature dependent with few taxa affected at 20°C, whereas substantial shifts occurred at 25°C and 30°C, indicating reduced microbial resilience at higher temperatures. OTC elevated total antimicrobial resistance gene (ARG) abundance, enriching tetracycline and β-lactam resistant genes consistent with co-selection. ARG-host linkages were diffuse at 20°C but consolidated within , , , and at 25°C and 30°C. Notably, OTC-induced dysbiosis persisted through the withdrawal period. These findings demonstrate that temperature modulates both the magnitude and persistence of OTC-driven microbiome disruption and resistome enrichment, underscoring the importance of temperature-aware antibiotic management to mitigate antimicrobial resistance risks and safeguard fish health and food safety in aquaculture. This study reveals that water temperature critically shapes how antibiotics affect the gut microbiome and antimicrobial resistance in channel catfish. Metagenomic sequencing results showed that oxytetracycline (OTC) treatment caused minimal disruption of the microbiome at 20°C, but induced significant community shifts and enrichment of antimicrobial resistance genes (ARGs) at 25°C and 30°C. Higher temperatures reduced microbial resilience, consolidating ARGs within key bacterial genera such as and . Importantly, OTC-induced microbiome changes and resistance persisted through the withdrawal period. These findings highlight temperature as a major driver of antibiotic impact in aquaculture, emphasizing the prudent use of antibiotics at different disease breakout temperatures. |
| format | Artículo científico |
| id | pubmed_41910137 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Microbiology spectrum |
| record_format | pubmed |
| spellingShingle | Temperature modulates gut microbiome disruption and resistome enrichment in oxytetracycline-treated channel catfish (). Li, Xiran Wang, Hongye Abdelrahman, Hisham A Kelly, Anita M Roy, Luke A Soto, Esteban Wang, Luxin Temperature modulates gut microbiome disruption and resistome enrichment in oxytetracycline-treated channel catfish (). Li, Xiran Wang, Hongye Abdelrahman, Hisham A Kelly, Anita M Roy, Luke A Soto, Esteban Wang, Luxin Oxytetracycline (OTC) is one of the few antibiotics approved by the U.S. Food and Drug Administration for catfish aquaculture. Unfortunately, OTC resistance has been frequently detected in production environments, with the fish gut identified as a potential hotspot for resistance selection. In aquaculture systems, water temperature is a critical factor influencing fish physiology, antibiotic pharmacokinetics, and water resistome development. However, its role in modulating OTC effects on the fish gut microbiome remains underexplored. This study examined temperature-dependent microbiome and resistome responses in channel catfish () when treated with OTC at 20°C, 25°C, and 30°C. Gut contents collected at treatment completion and after withdrawal were analyzed via metagenomic sequencing. In untreated fish, temperature alone shaped microbial structure and function, with the Shannon diversity increasing with temperatures and the β-diversity differing significantly across temperature groups. After OTC exposure, microbial responses were markedly temperature dependent with few taxa affected at 20°C, whereas substantial shifts occurred at 25°C and 30°C, indicating reduced microbial resilience at higher temperatures. OTC elevated total antimicrobial resistance gene (ARG) abundance, enriching tetracycline and β-lactam resistant genes consistent with co-selection. ARG-host linkages were diffuse at 20°C but consolidated within , , , and at 25°C and 30°C. Notably, OTC-induced dysbiosis persisted through the withdrawal period. These findings demonstrate that temperature modulates both the magnitude and persistence of OTC-driven microbiome disruption and resistome enrichment, underscoring the importance of temperature-aware antibiotic management to mitigate antimicrobial resistance risks and safeguard fish health and food safety in aquaculture. This study reveals that water temperature critically shapes how antibiotics affect the gut microbiome and antimicrobial resistance in channel catfish. Metagenomic sequencing results showed that oxytetracycline (OTC) treatment caused minimal disruption of the microbiome at 20°C, but induced significant community shifts and enrichment of antimicrobial resistance genes (ARGs) at 25°C and 30°C. Higher temperatures reduced microbial resilience, consolidating ARGs within key bacterial genera such as and . Importantly, OTC-induced microbiome changes and resistance persisted through the withdrawal period. These findings highlight temperature as a major driver of antibiotic impact in aquaculture, emphasizing the prudent use of antibiotics at different disease breakout temperatures. |
| title | Temperature modulates gut microbiome disruption and resistome enrichment in oxytetracycline-treated channel catfish (). |
| url | https://pubmed.ncbi.nlm.nih.gov/41910137/ |