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Main Authors: Venetsianou, Nefeli Kleopatra, Paragkamian, Savvas, Kalaentzis, Konstantinos, Loukas, Alexios, Damianou, Christina, Lagani, Vincenzo, Jensen, Lars Juhl, Pafilis, Evangelos
Format: Artículo científico
Language:en
Published: Microbial ecology 2026
Online Access:https://pubmed.ncbi.nlm.nih.gov/41915167/
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author Venetsianou, Nefeli Kleopatra
Paragkamian, Savvas
Kalaentzis, Konstantinos
Loukas, Alexios
Damianou, Christina
Lagani, Vincenzo
Jensen, Lars Juhl
Pafilis, Evangelos
author_facet Venetsianou, Nefeli Kleopatra
Paragkamian, Savvas
Kalaentzis, Konstantinos
Loukas, Alexios
Damianou, Christina
Lagani, Vincenzo
Jensen, Lars Juhl
Pafilis, Evangelos
Venetsianou, Nefeli Kleopatra
Paragkamian, Savvas
Kalaentzis, Konstantinos
Loukas, Alexios
Damianou, Christina
Lagani, Vincenzo
Jensen, Lars Juhl
Pafilis, Evangelos
collection PubMed - marine biology
contents LLM-Assessed Relatedness of Microbiome Study Descriptions Aligns more Strongly with Functional than with Taxonomic Profile Similarity. Venetsianou, Nefeli Kleopatra Paragkamian, Savvas Kalaentzis, Konstantinos Loukas, Alexios Damianou, Christina Lagani, Vincenzo Jensen, Lars Juhl Pafilis, Evangelos Microbiome studies reveal the taxonomic and functional composition of microbial communities inhabiting many diverse environments. Comprehensive microbiome repositories, such as MGnify, organize data into studies, each consisting of multiple sequencing runs or assemblies and accompanying metadata. This structure enables integrative, large-scale, cross-study analyses, leading to broader insights across ecosystems, hosts, and experimental contexts. Despite extensive microbiome research, methods for defining similarity between studies and validating those similarity metrics, remain insufficiently established, especially for large-scale analyses. To address this, we evaluate whether taxonomic and functional similarities from MGnify can serve as reliable indicators of study relatedness between study pairs, testing multiple metrics against conceptual relatedness (e.g., shared environments, goals, or methods). To scale validation, we introduce a framework that applies a Large Language Model (LLM) to study descriptions, categorizing study pairs by relatedness. Our results show that functional similarity correlates more strongly with LLM-inferred study relatedness than taxonomic similarity, highlighting both the promise and limitations of current metrics. Via the above, we demonstrate the value of combining microbial profiles with LLM-driven semantic reasoning to navigate the expanding landscape of metagenomic research.
format Artículo científico
id pubmed_41915167
institution PubMed
language en
publishDate 2026
publisher Microbial ecology
record_format pubmed
spellingShingle LLM-Assessed Relatedness of Microbiome Study Descriptions Aligns more Strongly with Functional than with Taxonomic Profile Similarity.
Venetsianou, Nefeli Kleopatra
Paragkamian, Savvas
Kalaentzis, Konstantinos
Loukas, Alexios
Damianou, Christina
Lagani, Vincenzo
Jensen, Lars Juhl
Pafilis, Evangelos
LLM-Assessed Relatedness of Microbiome Study Descriptions Aligns more Strongly with Functional than with Taxonomic Profile Similarity. Venetsianou, Nefeli Kleopatra Paragkamian, Savvas Kalaentzis, Konstantinos Loukas, Alexios Damianou, Christina Lagani, Vincenzo Jensen, Lars Juhl Pafilis, Evangelos Microbiome studies reveal the taxonomic and functional composition of microbial communities inhabiting many diverse environments. Comprehensive microbiome repositories, such as MGnify, organize data into studies, each consisting of multiple sequencing runs or assemblies and accompanying metadata. This structure enables integrative, large-scale, cross-study analyses, leading to broader insights across ecosystems, hosts, and experimental contexts. Despite extensive microbiome research, methods for defining similarity between studies and validating those similarity metrics, remain insufficiently established, especially for large-scale analyses. To address this, we evaluate whether taxonomic and functional similarities from MGnify can serve as reliable indicators of study relatedness between study pairs, testing multiple metrics against conceptual relatedness (e.g., shared environments, goals, or methods). To scale validation, we introduce a framework that applies a Large Language Model (LLM) to study descriptions, categorizing study pairs by relatedness. Our results show that functional similarity correlates more strongly with LLM-inferred study relatedness than taxonomic similarity, highlighting both the promise and limitations of current metrics. Via the above, we demonstrate the value of combining microbial profiles with LLM-driven semantic reasoning to navigate the expanding landscape of metagenomic research.
title LLM-Assessed Relatedness of Microbiome Study Descriptions Aligns more Strongly with Functional than with Taxonomic Profile Similarity.
url https://pubmed.ncbi.nlm.nih.gov/41915167/