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author Bock, Samantha L
Lyons, Kady
Yang, Lei
Wyffels, Jennifer
Adams, Lance
Klerks, Nienke
Jeskie, Aaron
Leever, Ellen
Hartl, Taylor
Almunia, Javier
Peterson, Dan
Ferreira, Ana
Okamoto, Joe
Archibald, Kate
Spaulding, Chris
Leyden, Kayla
Montano, Gisele A
Vasey, Savannah
Rowland, Adrienne
Watson, Lise
Lopez, Alfonso
Hemdal, Jay
Stafford, Mike
Bronikowski, Anne
Naylor, Gavin J P
Parrott, Benjamin B
author_facet Bock, Samantha L
Lyons, Kady
Yang, Lei
Wyffels, Jennifer
Adams, Lance
Klerks, Nienke
Jeskie, Aaron
Leever, Ellen
Hartl, Taylor
Almunia, Javier
Peterson, Dan
Ferreira, Ana
Okamoto, Joe
Archibald, Kate
Spaulding, Chris
Leyden, Kayla
Montano, Gisele A
Vasey, Savannah
Rowland, Adrienne
Watson, Lise
Lopez, Alfonso
Hemdal, Jay
Stafford, Mike
Bronikowski, Anne
Naylor, Gavin J P
Parrott, Benjamin B
Bock, Samantha L
Lyons, Kady
Yang, Lei
Wyffels, Jennifer
Adams, Lance
Klerks, Nienke
Jeskie, Aaron
Leever, Ellen
Hartl, Taylor
Almunia, Javier
Peterson, Dan
Ferreira, Ana
Okamoto, Joe
Archibald, Kate
Spaulding, Chris
Leyden, Kayla
Montano, Gisele A
Vasey, Savannah
Rowland, Adrienne
Watson, Lise
Lopez, Alfonso
Hemdal, Jay
Stafford, Mike
Bronikowski, Anne
Naylor, Gavin J P
Parrott, Benjamin B
collection PubMed - marine biology
contents Genome-Wide DNA Methylation Patterns Predict Age in the Zebra Shark (Stegostoma tigrinum) and Provide Insight Into the Evolution of Vertebrate Aging. Bock, Samantha L Lyons, Kady Yang, Lei Wyffels, Jennifer Adams, Lance Klerks, Nienke Jeskie, Aaron Leever, Ellen Hartl, Taylor Almunia, Javier Peterson, Dan Ferreira, Ana Okamoto, Joe Archibald, Kate Spaulding, Chris Leyden, Kayla Montano, Gisele A Vasey, Savannah Rowland, Adrienne Watson, Lise Lopez, Alfonso Hemdal, Jay Stafford, Mike Bronikowski, Anne Naylor, Gavin J P Parrott, Benjamin B Animals DNA Methylation Sharks Aging Epigenesis, Genetic Biological Evolution Genome Epigenomic changes are a hallmark of aging, and DNA methylation (DNAm) has emerged as the most reliable molecular marker of an individual's age. Genome-wide patterns of age-associated hypo- and hypermethylation have been applied to generate predictive models (i.e., "epigenetic clocks") capable of estimating chronological age in an increasingly diverse set of species including many mammals, a few birds, a reptile, and several bony fishes. Elasmobranchs (sharks, skates, and rays) are underrepresented in comparative investigations of epigenetic aging despite exhibiting exceptional life history variation, occupying a key basal position in the vertebrate phylogeny, and encompassing a large proportion of threatened species lacking accurate, non-lethal age determination methods. Here, we characterize epigenome-wide aging signals in the zebra shark (Stegostoma tigrinum), a long-lived elasmobranch of conservation concern, from whole-genome enzymatic methyl-sequencing of whole blood. Using a cohort of 51 known-age aquarium-bred individuals, we develop several epigenetic clock models capable of predicting chronological age with a median absolute error of 1.03-1.99 years (3.32%-6.42% of lifespan) based on the methylation status of as few as ten cytosines. We further apply our models to 19 individuals of unknown age originating from the wild. By profiling the broader age-associated methylome we demonstrate that these patterns not only predict age with high accuracy but also exhibit striking similarities in their genomic distributions to those observed in mammals pointing to conservation of the processes underlying epigenetic aging across vertebrates.
format Artículo científico
id pubmed_41930734
institution PubMed
language en
publishDate 2026
publisher Molecular ecology
record_format pubmed
spellingShingle Genome-Wide DNA Methylation Patterns Predict Age in the Zebra Shark (Stegostoma tigrinum) and Provide Insight Into the Evolution of Vertebrate Aging.
Bock, Samantha L
Lyons, Kady
Yang, Lei
Wyffels, Jennifer
Adams, Lance
Klerks, Nienke
Jeskie, Aaron
Leever, Ellen
Hartl, Taylor
Almunia, Javier
Peterson, Dan
Ferreira, Ana
Okamoto, Joe
Archibald, Kate
Spaulding, Chris
Leyden, Kayla
Montano, Gisele A
Vasey, Savannah
Rowland, Adrienne
Watson, Lise
Lopez, Alfonso
Hemdal, Jay
Stafford, Mike
Bronikowski, Anne
Naylor, Gavin J P
Parrott, Benjamin B
Animals
DNA Methylation
Sharks
Aging
Epigenesis, Genetic
Biological Evolution
Genome
Genome-Wide DNA Methylation Patterns Predict Age in the Zebra Shark (Stegostoma tigrinum) and Provide Insight Into the Evolution of Vertebrate Aging. Bock, Samantha L Lyons, Kady Yang, Lei Wyffels, Jennifer Adams, Lance Klerks, Nienke Jeskie, Aaron Leever, Ellen Hartl, Taylor Almunia, Javier Peterson, Dan Ferreira, Ana Okamoto, Joe Archibald, Kate Spaulding, Chris Leyden, Kayla Montano, Gisele A Vasey, Savannah Rowland, Adrienne Watson, Lise Lopez, Alfonso Hemdal, Jay Stafford, Mike Bronikowski, Anne Naylor, Gavin J P Parrott, Benjamin B Animals DNA Methylation Sharks Aging Epigenesis, Genetic Biological Evolution Genome Epigenomic changes are a hallmark of aging, and DNA methylation (DNAm) has emerged as the most reliable molecular marker of an individual's age. Genome-wide patterns of age-associated hypo- and hypermethylation have been applied to generate predictive models (i.e., "epigenetic clocks") capable of estimating chronological age in an increasingly diverse set of species including many mammals, a few birds, a reptile, and several bony fishes. Elasmobranchs (sharks, skates, and rays) are underrepresented in comparative investigations of epigenetic aging despite exhibiting exceptional life history variation, occupying a key basal position in the vertebrate phylogeny, and encompassing a large proportion of threatened species lacking accurate, non-lethal age determination methods. Here, we characterize epigenome-wide aging signals in the zebra shark (Stegostoma tigrinum), a long-lived elasmobranch of conservation concern, from whole-genome enzymatic methyl-sequencing of whole blood. Using a cohort of 51 known-age aquarium-bred individuals, we develop several epigenetic clock models capable of predicting chronological age with a median absolute error of 1.03-1.99 years (3.32%-6.42% of lifespan) based on the methylation status of as few as ten cytosines. We further apply our models to 19 individuals of unknown age originating from the wild. By profiling the broader age-associated methylome we demonstrate that these patterns not only predict age with high accuracy but also exhibit striking similarities in their genomic distributions to those observed in mammals pointing to conservation of the processes underlying epigenetic aging across vertebrates.
title Genome-Wide DNA Methylation Patterns Predict Age in the Zebra Shark (Stegostoma tigrinum) and Provide Insight Into the Evolution of Vertebrate Aging.
topic Animals
DNA Methylation
Sharks
Aging
Epigenesis, Genetic
Biological Evolution
Genome
url https://pubmed.ncbi.nlm.nih.gov/41930734/