Salvato in:
| Autori principali: | , , |
|---|---|
| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Parasites, hosts and diseases
2026
|
| Soggetti: | |
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/41937381/ |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
| _version_ | 1868266064355262464 |
|---|---|
| author | Hong, Hyun Ki Park, Youngjun Shin, Sang Phil |
| author_facet | Hong, Hyun Ki Park, Youngjun Shin, Sang Phil Hong, Hyun Ki Park, Youngjun Shin, Sang Phil |
| collection | PubMed - marine biology |
| contents | Purification of Kudoa septempunctata myxospores by using trypsin and fluorescence activated cell sorting. Hong, Hyun Ki Park, Youngjun Shin, Sang Phil Myxozoa Flow Cytometry Animals Trypsin Spores Flounder Fish Diseases Centrifugation, Density Gradient Kudoa septempunctata, a myxozoan, has been identified as the causative agent of foodborne illnesses associated with the consumption of raw olive flounder. To develop effective control methods against this parasite, fundamental research-including viability determination, transcriptome analysis, and antigenicity assessment of K. septempunctata myxospores-is required. This research necessitates the purification of the parasites. Sequential trypsin digestion, followed by density gradient purification, was performed to isolate the K. septempunctata myxospores. Further purification was achieved through fluorescence-activated cell sorting at a concentration of 106 to 107 myxospores/ml. The results demonstrated that the combination of trypsin digestion and density gradient methods consistently produced approximately 40 times more viable myxospores than the density gradient method alone. Additionally, the fluorescence-activated cell sorting method enhanced the purity of the myxospores by approximately 10%. The procedures described in this study will support research (such as RNA-sequencing, proteomics, vaccine antigen preparation) aimed at developing control methods for K. septempunctata including the fundamental research. |
| format | Artículo científico |
| id | pubmed_41937381 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Parasites, hosts and diseases |
| record_format | pubmed |
| spellingShingle | Purification of Kudoa septempunctata myxospores by using trypsin and fluorescence activated cell sorting. Hong, Hyun Ki Park, Youngjun Shin, Sang Phil Myxozoa Flow Cytometry Animals Trypsin Spores Flounder Fish Diseases Centrifugation, Density Gradient Purification of Kudoa septempunctata myxospores by using trypsin and fluorescence activated cell sorting. Hong, Hyun Ki Park, Youngjun Shin, Sang Phil Myxozoa Flow Cytometry Animals Trypsin Spores Flounder Fish Diseases Centrifugation, Density Gradient Kudoa septempunctata, a myxozoan, has been identified as the causative agent of foodborne illnesses associated with the consumption of raw olive flounder. To develop effective control methods against this parasite, fundamental research-including viability determination, transcriptome analysis, and antigenicity assessment of K. septempunctata myxospores-is required. This research necessitates the purification of the parasites. Sequential trypsin digestion, followed by density gradient purification, was performed to isolate the K. septempunctata myxospores. Further purification was achieved through fluorescence-activated cell sorting at a concentration of 106 to 107 myxospores/ml. The results demonstrated that the combination of trypsin digestion and density gradient methods consistently produced approximately 40 times more viable myxospores than the density gradient method alone. Additionally, the fluorescence-activated cell sorting method enhanced the purity of the myxospores by approximately 10%. The procedures described in this study will support research (such as RNA-sequencing, proteomics, vaccine antigen preparation) aimed at developing control methods for K. septempunctata including the fundamental research. |
| title | Purification of Kudoa septempunctata myxospores by using trypsin and fluorescence activated cell sorting. |
| topic | Myxozoa Flow Cytometry Animals Trypsin Spores Flounder Fish Diseases Centrifugation, Density Gradient |
| url | https://pubmed.ncbi.nlm.nih.gov/41937381/ |