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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Animal reproduction science
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41955652/ |
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Table of Contents:
- Cryopreservation and xenotransplantation of spotted sea bass (Lateolabrax maculatus) spermatogonia. Guo, Jieyun Yan, Lulu Zhao, Chao Zhang, Bo Lin, Jiangtian Qiu, Lihua Li, Shengkang Animals Cryopreservation Male Spermatogonia Bass Transplantation, Heterologous Semen Preservation Cryopreservation of spermatogonia (SGs) is crucial for aquaculture, broodstock innovation, and the long-term conservation of aquatic genetic resources. This study developed an efficient cryopreservation protocol for spotted sea bass SGs and evaluated their post-thaw viability and transplantation potential. Initially, we characterized testicular tissue from 6-month-old male spotted sea bass, identifying various germ cell stages and strong Lmvasa expression in SGs. An optimized method for isolating and enriching SGs using Percoll density gradient centrifugation yielded a highly viable (99.6%) SG population, confirmed by morphology and germline markers (Lmvasa, Lmdnd, Lmnanos3). Subsequently, a cryopreservation protocol was optimized, with the optimal cryomedia comprising 1.3 M DMSO + 0.1 M Trehalose + 10% FBS + 35.2% extender in MEM, achieving a remarkable post-thaw SG viability of 91.83%. The transplantation potential of both fresh and 1-year cryopreserved SGs was evaluated by xenotransplantation into medaka larvae. Both fresh and 1-year cryopreserved SGs successfully colonized the genital ridges and formed germline chimeras, with 25% chimerism in fresh SGs and 20% in cryopreserved SGs, as confirmed by donor-specific Lmvasa 3'UTR PCR analysis. This study establishes a comprehensive system for SG isolation, cryopreservation, and functional assessment, providing a foundational tool for marine germplasm banking and surrogate broodstock technology.