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| Main Authors: | , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Journal of hazardous materials
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41966559/ |
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| _version_ | 1868266061980237825 |
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| author | Jordán, Maria Bustos-Caparros, Esteban Gago, Juan F Zhang, Zhenfa Tian, Zhenyu Singleton, David R Rossello-Mora, Ramon Grifoll, Magdalena Vila, Joaquim |
| author_facet | Jordán, Maria Bustos-Caparros, Esteban Gago, Juan F Zhang, Zhenfa Tian, Zhenyu Singleton, David R Rossello-Mora, Ramon Grifoll, Magdalena Vila, Joaquim Jordán, Maria Bustos-Caparros, Esteban Gago, Juan F Zhang, Zhenfa Tian, Zhenyu Singleton, David R Rossello-Mora, Ramon Grifoll, Magdalena Vila, Joaquim |
| collection | PubMed - marine biology |
| contents | Unraveling acridine degradation mechanisms in PAH-contaminated soils using DNA-SIP combined with metagenomics and soil transcriptomics. Jordán, Maria Bustos-Caparros, Esteban Gago, Juan F Zhang, Zhenfa Tian, Zhenyu Singleton, David R Rossello-Mora, Ramon Grifoll, Magdalena Vila, Joaquim Soil Pollutants Metagenomics Biodegradation, Environmental Acridines Soil Microbiology Polycyclic Aromatic Hydrocarbons RNA, Ribosomal, 16S Transcriptome Polycyclic aromatic nitrogen heterocycles (PANHs), also known as azaarenes, are common co-contaminants at sites contaminated with polycyclic aromatic hydrocarbons (PAHs). Recent non-target analysis of PAH-contaminated soil samples has revealed an unexpected abundance and diversity of PANHs, with acridine standing out as a predominant compound within this group. Despite its known toxicity and prevalence in contaminated soils, the microbial communities and biochemical mechanisms responsible for acridine degradation remain poorly understood. We conducted DNA-stable isotope probing (DNA-SIP) using newly synthesized uniformly labeled C-acridine to comprehensively assess the bacterial taxa and functional genes involved in acridine biodegradation in a creosote-contaminated soil. Metagenomic analysis of C-enriched DNA from soil incubations identified a member of the genus Sphingobium as the primary acridine degrader. Transcriptomic analysis based on its 16S rRNA gene expression demonstrated a strong correlation with acridine removal from the soil. Shotgun metagenomic sequencing enabled the reconstruction of one metagenome-assembled genome (MAG). Functional annotation of this MAG revealed five gene clusters potentially involved in acridine biodegradation, and their actual contribution was assessed by gene expression analysis in soil incubations. Based on these findings, we reconstructed the metabolic pathway for putative acridine degradation in PAH-contaminated soil. |
| format | Artículo científico |
| id | pubmed_41966559 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Journal of hazardous materials |
| record_format | pubmed |
| spellingShingle | Unraveling acridine degradation mechanisms in PAH-contaminated soils using DNA-SIP combined with metagenomics and soil transcriptomics. Jordán, Maria Bustos-Caparros, Esteban Gago, Juan F Zhang, Zhenfa Tian, Zhenyu Singleton, David R Rossello-Mora, Ramon Grifoll, Magdalena Vila, Joaquim Soil Pollutants Metagenomics Biodegradation, Environmental Acridines Soil Microbiology Polycyclic Aromatic Hydrocarbons RNA, Ribosomal, 16S Transcriptome Unraveling acridine degradation mechanisms in PAH-contaminated soils using DNA-SIP combined with metagenomics and soil transcriptomics. Jordán, Maria Bustos-Caparros, Esteban Gago, Juan F Zhang, Zhenfa Tian, Zhenyu Singleton, David R Rossello-Mora, Ramon Grifoll, Magdalena Vila, Joaquim Soil Pollutants Metagenomics Biodegradation, Environmental Acridines Soil Microbiology Polycyclic Aromatic Hydrocarbons RNA, Ribosomal, 16S Transcriptome Polycyclic aromatic nitrogen heterocycles (PANHs), also known as azaarenes, are common co-contaminants at sites contaminated with polycyclic aromatic hydrocarbons (PAHs). Recent non-target analysis of PAH-contaminated soil samples has revealed an unexpected abundance and diversity of PANHs, with acridine standing out as a predominant compound within this group. Despite its known toxicity and prevalence in contaminated soils, the microbial communities and biochemical mechanisms responsible for acridine degradation remain poorly understood. We conducted DNA-stable isotope probing (DNA-SIP) using newly synthesized uniformly labeled C-acridine to comprehensively assess the bacterial taxa and functional genes involved in acridine biodegradation in a creosote-contaminated soil. Metagenomic analysis of C-enriched DNA from soil incubations identified a member of the genus Sphingobium as the primary acridine degrader. Transcriptomic analysis based on its 16S rRNA gene expression demonstrated a strong correlation with acridine removal from the soil. Shotgun metagenomic sequencing enabled the reconstruction of one metagenome-assembled genome (MAG). Functional annotation of this MAG revealed five gene clusters potentially involved in acridine biodegradation, and their actual contribution was assessed by gene expression analysis in soil incubations. Based on these findings, we reconstructed the metabolic pathway for putative acridine degradation in PAH-contaminated soil. |
| title | Unraveling acridine degradation mechanisms in PAH-contaminated soils using DNA-SIP combined with metagenomics and soil transcriptomics. |
| topic | Soil Pollutants Metagenomics Biodegradation, Environmental Acridines Soil Microbiology Polycyclic Aromatic Hydrocarbons RNA, Ribosomal, 16S Transcriptome |
| url | https://pubmed.ncbi.nlm.nih.gov/41966559/ |