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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Fish & shellfish immunology
2026
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| Soggetti: | |
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/42000086/ |
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| _version_ | 1868266059504549888 |
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| author | Chen, Yunong Zhang, Zhixing Xu, XiaoJin Chen, Jianxiang Zhang, Le Wu, Changmao Xiao, Yujin Sha, Yuxuan Guo, Minglan |
| author_facet | Chen, Yunong Zhang, Zhixing Xu, XiaoJin Chen, Jianxiang Zhang, Le Wu, Changmao Xiao, Yujin Sha, Yuxuan Guo, Minglan Chen, Yunong Zhang, Zhixing Xu, XiaoJin Chen, Jianxiang Zhang, Le Wu, Changmao Xiao, Yujin Sha, Yuxuan Guo, Minglan |
| collection | PubMed - marine biology |
| contents | Influence of the two-component system PhoBR associated with phosphate-sensing capacity on the virulence mechanism of Vibrio alginolyticus. Chen, Yunong Zhang, Zhixing Xu, XiaoJin Chen, Jianxiang Zhang, Le Wu, Changmao Xiao, Yujin Sha, Yuxuan Guo, Minglan Vibrio alginolyticus Virulence Bacterial Proteins Phosphates Animals Biofilms Gene Expression Regulation, Bacterial Vibrio Infections Vibrio alginolyticus is an opportunistic pathogen responsible for serious diseases in fish and shrimp, leading to severe economic losses in aquaculture. Although many virulence factors of V. alginolyticus have been identified, the regulatory roles of the phosphate-regulation genes phoB and phoR in its virulence have not yet been reported. In this study, stable silencing strains, phoB-RNAi and phoR-RNAi, were constructed to evaluate their contributions to bacterial pathogenicity. Previous studies demonstrated that that Scutellaria baicalensis (8 mg/mL) significantly inhibited on the growth, motility and biofilm formation ability of V. alginolyticus ND-01 (OD = 0.5) after 12 h of exposure, while also significantly affecting the expression levels of multiple genes, including phoB and phoR. The phoB-RNAi and phoR-RNAi strains were respectively constructed using RNAi, respectively. Biological characteristics experiments showed that, compared with wild-type strains, silencing phoB and phoR significantly reduced biofilm formation ability (82.44% and 83.58%, respectively), adhesion (74.28% and 95.48%, respectively), chemotaxis (86.48% and 78.60%, respectively), and motility (31.66% and 12.18%, respectively). Transcriptomic analysis of phoR-RNAi identified 2095 differentially expressed genes, including those involved in the two-component system, ABC transporters, and bacterial secretion pathways. Artificial infection experiments further proved that PhoR-RNAi significantly reduced bacterial virulence. Compared with wild-type V. alginolyticus, the total cumulative mortality rate of Epinephelus coioid decreased by 70%. Overall, these results indicate that phoB and phoR play key roles in regulating the virulence mechanism of V. alginolyticus by modulating its adhesion, motility, chemotaxis, and biofilm formation ability. In addition, they mediate environmental phosphate responsiveness. These findings provide new insights for developing preventive strategies in aquaculture. |
| format | Artículo científico |
| id | pubmed_42000086 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | Influence of the two-component system PhoBR associated with phosphate-sensing capacity on the virulence mechanism of Vibrio alginolyticus. Chen, Yunong Zhang, Zhixing Xu, XiaoJin Chen, Jianxiang Zhang, Le Wu, Changmao Xiao, Yujin Sha, Yuxuan Guo, Minglan Vibrio alginolyticus Virulence Bacterial Proteins Phosphates Animals Biofilms Gene Expression Regulation, Bacterial Vibrio Infections Influence of the two-component system PhoBR associated with phosphate-sensing capacity on the virulence mechanism of Vibrio alginolyticus. Chen, Yunong Zhang, Zhixing Xu, XiaoJin Chen, Jianxiang Zhang, Le Wu, Changmao Xiao, Yujin Sha, Yuxuan Guo, Minglan Vibrio alginolyticus Virulence Bacterial Proteins Phosphates Animals Biofilms Gene Expression Regulation, Bacterial Vibrio Infections Vibrio alginolyticus is an opportunistic pathogen responsible for serious diseases in fish and shrimp, leading to severe economic losses in aquaculture. Although many virulence factors of V. alginolyticus have been identified, the regulatory roles of the phosphate-regulation genes phoB and phoR in its virulence have not yet been reported. In this study, stable silencing strains, phoB-RNAi and phoR-RNAi, were constructed to evaluate their contributions to bacterial pathogenicity. Previous studies demonstrated that that Scutellaria baicalensis (8 mg/mL) significantly inhibited on the growth, motility and biofilm formation ability of V. alginolyticus ND-01 (OD = 0.5) after 12 h of exposure, while also significantly affecting the expression levels of multiple genes, including phoB and phoR. The phoB-RNAi and phoR-RNAi strains were respectively constructed using RNAi, respectively. Biological characteristics experiments showed that, compared with wild-type strains, silencing phoB and phoR significantly reduced biofilm formation ability (82.44% and 83.58%, respectively), adhesion (74.28% and 95.48%, respectively), chemotaxis (86.48% and 78.60%, respectively), and motility (31.66% and 12.18%, respectively). Transcriptomic analysis of phoR-RNAi identified 2095 differentially expressed genes, including those involved in the two-component system, ABC transporters, and bacterial secretion pathways. Artificial infection experiments further proved that PhoR-RNAi significantly reduced bacterial virulence. Compared with wild-type V. alginolyticus, the total cumulative mortality rate of Epinephelus coioid decreased by 70%. Overall, these results indicate that phoB and phoR play key roles in regulating the virulence mechanism of V. alginolyticus by modulating its adhesion, motility, chemotaxis, and biofilm formation ability. In addition, they mediate environmental phosphate responsiveness. These findings provide new insights for developing preventive strategies in aquaculture. |
| title | Influence of the two-component system PhoBR associated with phosphate-sensing capacity on the virulence mechanism of Vibrio alginolyticus. |
| topic | Vibrio alginolyticus Virulence Bacterial Proteins Phosphates Animals Biofilms Gene Expression Regulation, Bacterial Vibrio Infections |
| url | https://pubmed.ncbi.nlm.nih.gov/42000086/ |