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| Format: | Artículo científico |
| Language: | en |
| Published: |
Archives of microbiology
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/42008016/ |
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Table of Contents:
- Comprehensive identification and subcellular localization prediction of carbonic anhydrases in the marine haptophyte Tisochrysis lutea based on a refined genome annotation. Yoneda, Kohei Carbonic Anhydrases Haptophyta Molecular Sequence Annotation Proteome Genome Computational Biology Photosynthesis The marine haptophyte Tisochrysis lutea is a commercially important microalga known for its production of high-value lipids and carotenoids. While the CO-concentrating mechanism (CCM) is essential for efficient photosynthesis in marine environments, its molecular basis in haptophytes remains poorly understood despite their ecological and industrial importance. In the present study, I first assessed proteome completeness of the latest gene models of T. lutea and subsequently re-annotated the reference genome to refine these models. I then identified the complete repertoire of carbonic anhydrases (CAs). The re-annotated models significantly improved the proteome-level BUSCO completeness from 76.7% to 89.9% and resolved previously fragmented sequences, resulting in 17,205 protein-coding genes with markedly reduced fragmentation. Within this refined proteome, I identified 16 CA genes across six distinct classes: one α-, five β-, three γ-, two δ-, one θ-, and four ι-type CAs. Subcellular localization was predicted using an integrated bioinformatic pipeline (SignalP, TargetP, ASAFind2, HECTAR, and DeepLoc-2), which suggested a complex compartmentalization of inorganic carbon interconversion. Notably, TlδCA1 and TlιCA3 exhibited high expression levels and were predicted to localize to the plasma membrane and plastid, respectively, indicating their pivotal roles in the CCM of T. lutea. Furthermore, structural analysis revealed unique features in haptophyte CAs, such as specific transmembrane domain configuration in δ-CA and conserved domain repeats in ι-CAs. These findings provide a molecular framework for understanding the CCM machinery in T. lutea, offering a robust genomic platform for future metabolic engineering aimed at enhancing carbon fixation efficiency in marine haptophytes.