Robust SERS Platform Enabled by Silver-Nanoparticle-Loaded Hydrogel Millibeads for Trace Detection in High-Salt and Protein-Rich Complex Matrixes.
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| Format: | Artículo científico |
| Sprache: | en |
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2026
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| author | Su, Xiaotong Liu, Ke Zhang, Zhiyang Shen, Qirui Feng, Yu Chen, Yan Sui, Yifan Chen, Jiadong Liu, Renshuang Zhou, Haoyuan Wu, Yanzhou Choo, Jaebum Chen, Lingxin |
| author_facet | Su, Xiaotong Liu, Ke Zhang, Zhiyang Shen, Qirui Feng, Yu Chen, Yan Sui, Yifan Chen, Jiadong Liu, Renshuang Zhou, Haoyuan Wu, Yanzhou Choo, Jaebum Chen, Lingxin Su, Xiaotong Liu, Ke Zhang, Zhiyang Shen, Qirui Feng, Yu Chen, Yan Sui, Yifan Chen, Jiadong Liu, Renshuang Zhou, Haoyuan Wu, Yanzhou Choo, Jaebum Chen, Lingxin |
| collection | PubMed - marine biology |
| contents | Robust SERS Platform Enabled by Silver-Nanoparticle-Loaded Hydrogel Millibeads for Trace Detection in High-Salt and Protein-Rich Complex Matrixes. Su, Xiaotong Liu, Ke Zhang, Zhiyang Shen, Qirui Feng, Yu Chen, Yan Sui, Yifan Chen, Jiadong Liu, Renshuang Zhou, Haoyuan Wu, Yanzhou Choo, Jaebum Chen, Lingxin Silver Metal Nanoparticles Spectrum Analysis, Raman Hydrogels Animals Sodium Chloride Serum Albumin, Bovine Seawater Reliable trace detection in complex matrixes (e.g., seawater, biofluids) is challenging for conventional surface-enhanced Raman scattering (SERS) platforms due to salt-induced aggregation, protein adsorption, and coffee-ring effects. We present a robust SERS platform using evaporative shrinkage of a silver-nanoparticle (AgNP)-loaded hydrogel millibead (Ag@Gel-MB). The hydrogel millibead selectively enriches analytes while excluding proteins, and its subsequent shrinkage coconcentrates analytes and AgNPs into ultrasmall volumes to form dense hotspots, while the 3D network prevents salt-induced aggregation. Using Nile Blue, we achieved high sensitivity (LOD < 10 M), excellent uniformity (RSD ∼ 4.36%), and batch reproducibility (RSD ∼ 4.39%). Unlike droplet methods needing superhydrophobic surfaces, our platform avoids the coffee-ring effect on any support and achieves 15-fold and 100-fold improvements in salt and protein tolerance (150 mM NaCl, 100 μg/mL BSA) over conventional methods. It successfully detected malachite green in seawater and fish serum, demonstrating its practical reliability for the analysis of complex samples. |
| format | Artículo científico |
| id | pubmed_42010901 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Nano letters |
| record_format | pubmed |
| spellingShingle | Robust SERS Platform Enabled by Silver-Nanoparticle-Loaded Hydrogel Millibeads for Trace Detection in High-Salt and Protein-Rich Complex Matrixes. Su, Xiaotong Liu, Ke Zhang, Zhiyang Shen, Qirui Feng, Yu Chen, Yan Sui, Yifan Chen, Jiadong Liu, Renshuang Zhou, Haoyuan Wu, Yanzhou Choo, Jaebum Chen, Lingxin Silver Metal Nanoparticles Spectrum Analysis, Raman Hydrogels Animals Sodium Chloride Serum Albumin, Bovine Seawater Robust SERS Platform Enabled by Silver-Nanoparticle-Loaded Hydrogel Millibeads for Trace Detection in High-Salt and Protein-Rich Complex Matrixes. Su, Xiaotong Liu, Ke Zhang, Zhiyang Shen, Qirui Feng, Yu Chen, Yan Sui, Yifan Chen, Jiadong Liu, Renshuang Zhou, Haoyuan Wu, Yanzhou Choo, Jaebum Chen, Lingxin Silver Metal Nanoparticles Spectrum Analysis, Raman Hydrogels Animals Sodium Chloride Serum Albumin, Bovine Seawater Reliable trace detection in complex matrixes (e.g., seawater, biofluids) is challenging for conventional surface-enhanced Raman scattering (SERS) platforms due to salt-induced aggregation, protein adsorption, and coffee-ring effects. We present a robust SERS platform using evaporative shrinkage of a silver-nanoparticle (AgNP)-loaded hydrogel millibead (Ag@Gel-MB). The hydrogel millibead selectively enriches analytes while excluding proteins, and its subsequent shrinkage coconcentrates analytes and AgNPs into ultrasmall volumes to form dense hotspots, while the 3D network prevents salt-induced aggregation. Using Nile Blue, we achieved high sensitivity (LOD < 10 M), excellent uniformity (RSD ∼ 4.36%), and batch reproducibility (RSD ∼ 4.39%). Unlike droplet methods needing superhydrophobic surfaces, our platform avoids the coffee-ring effect on any support and achieves 15-fold and 100-fold improvements in salt and protein tolerance (150 mM NaCl, 100 μg/mL BSA) over conventional methods. It successfully detected malachite green in seawater and fish serum, demonstrating its practical reliability for the analysis of complex samples. |
| title | Robust SERS Platform Enabled by Silver-Nanoparticle-Loaded Hydrogel Millibeads for Trace Detection in High-Salt and Protein-Rich Complex Matrixes. |
| topic | Silver Metal Nanoparticles Spectrum Analysis, Raman Hydrogels Animals Sodium Chloride Serum Albumin, Bovine Seawater |
| url | https://pubmed.ncbi.nlm.nih.gov/42010901/ |