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| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Nature biotechnology
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/42020848/ |
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| _version_ | 1868266056195244034 |
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| author | Dehghannasiri, Roozbeh Kokot, Marek Starr, Alexander L Maziarz, Jamie Gordon, Tal Tan, Serena Y Wang, Peter L Voskoboynik, Ayelet Musser, Jacob M Deorowicz, Sebastian Salzman, Julia |
| author_facet | Dehghannasiri, Roozbeh Kokot, Marek Starr, Alexander L Maziarz, Jamie Gordon, Tal Tan, Serena Y Wang, Peter L Voskoboynik, Ayelet Musser, Jacob M Deorowicz, Sebastian Salzman, Julia Dehghannasiri, Roozbeh Kokot, Marek Starr, Alexander L Maziarz, Jamie Gordon, Tal Tan, Serena Y Wang, Peter L Voskoboynik, Ayelet Musser, Jacob M Deorowicz, Sebastian Salzman, Julia |
| collection | PubMed - marine biology |
| contents | Reference-free discovery with barcoded single-cell sequencing. Dehghannasiri, Roozbeh Kokot, Marek Starr, Alexander L Maziarz, Jamie Gordon, Tal Tan, Serena Y Wang, Peter L Voskoboynik, Ayelet Musser, Jacob M Deorowicz, Sebastian Salzman, Julia Single-cell RNA sequencing (scRNA-seq) analyses typically focus on alignment and differential gene expression, being blind to other transcriptome variation. Here we present sc-SPLASH for statistics-first, reference-free discovery on barcoded scRNA-seq and spatial transcriptomics; its independent BKC submodule optimizes barcoded data preprocessing and is approximately 50-fold faster than UMI-tools. sc-SPLASH discovers secreted repeat proteins in immune-like cells of sponge (Spongilla; missing from the reference) and tunicate (Ciona). |
| format | Artículo científico |
| id | pubmed_42020848 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Nature biotechnology |
| record_format | pubmed |
| spellingShingle | Reference-free discovery with barcoded single-cell sequencing. Dehghannasiri, Roozbeh Kokot, Marek Starr, Alexander L Maziarz, Jamie Gordon, Tal Tan, Serena Y Wang, Peter L Voskoboynik, Ayelet Musser, Jacob M Deorowicz, Sebastian Salzman, Julia Reference-free discovery with barcoded single-cell sequencing. Dehghannasiri, Roozbeh Kokot, Marek Starr, Alexander L Maziarz, Jamie Gordon, Tal Tan, Serena Y Wang, Peter L Voskoboynik, Ayelet Musser, Jacob M Deorowicz, Sebastian Salzman, Julia Single-cell RNA sequencing (scRNA-seq) analyses typically focus on alignment and differential gene expression, being blind to other transcriptome variation. Here we present sc-SPLASH for statistics-first, reference-free discovery on barcoded scRNA-seq and spatial transcriptomics; its independent BKC submodule optimizes barcoded data preprocessing and is approximately 50-fold faster than UMI-tools. sc-SPLASH discovers secreted repeat proteins in immune-like cells of sponge (Spongilla; missing from the reference) and tunicate (Ciona). |
| title | Reference-free discovery with barcoded single-cell sequencing. |
| url | https://pubmed.ncbi.nlm.nih.gov/42020848/ |