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| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Nature biotechnology
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/42020848/ |
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Table of Contents:
- Reference-free discovery with barcoded single-cell sequencing. Dehghannasiri, Roozbeh Kokot, Marek Starr, Alexander L Maziarz, Jamie Gordon, Tal Tan, Serena Y Wang, Peter L Voskoboynik, Ayelet Musser, Jacob M Deorowicz, Sebastian Salzman, Julia Single-cell RNA sequencing (scRNA-seq) analyses typically focus on alignment and differential gene expression, being blind to other transcriptome variation. Here we present sc-SPLASH for statistics-first, reference-free discovery on barcoded scRNA-seq and spatial transcriptomics; its independent BKC submodule optimizes barcoded data preprocessing and is approximately 50-fold faster than UMI-tools. sc-SPLASH discovers secreted repeat proteins in immune-like cells of sponge (Spongilla; missing from the reference) and tunicate (Ciona).