Enhanced Expression of CCD2 Enables Highly Efficient Crocetin Production in .
Fuente:
PubMed
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Sprache: | en |
| Veröffentlicht: |
Journal of agricultural and food chemistry
2026
|
| Schlagworte: | |
| Online-Zugang: | |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| _version_ | 1868266056192098305 |
|---|---|
| author | Guo, Guiping Chen, Huibin Wang, Hailin Zhang, Guilin Fu, Xiaodan Lee, Man Yuen Leung, Yun-Chung Li, Ningyang Liu, Zhen |
| author_facet | Guo, Guiping Chen, Huibin Wang, Hailin Zhang, Guilin Fu, Xiaodan Lee, Man Yuen Leung, Yun-Chung Li, Ningyang Liu, Zhen Guo, Guiping Chen, Huibin Wang, Hailin Zhang, Guilin Fu, Xiaodan Lee, Man Yuen Leung, Yun-Chung Li, Ningyang Liu, Zhen |
| collection | PubMed - marine biology |
| contents | Enhanced Expression of CCD2 Enables Highly Efficient Crocetin Production in . Guo, Guiping Chen, Huibin Wang, Hailin Zhang, Guilin Fu, Xiaodan Lee, Man Yuen Leung, Yun-Chung Li, Ningyang Liu, Zhen Yarrowia Carotenoids Vitamin A Fungal Proteins Fermentation Metabolic Engineering Dioxygenases Crocetin, a bioactive carotenoid derivative from saffron, exhibits antioxidant, anti-inflammatory, and metabolic regulatory properties. Its low natural abundance and limited extraction production hinder large-scale production. Microbial synthesis offers a promising alternative but is constrained by low carotenoid cleavage dioxygenase (CCD2) expression. Here, we engineered a zeaxanthin-producing strain to enhance CCD2 expression via fermentation temperature optimization, SUMOstar fusion, subcellular localization, and precursor supply improvement. The engineered strain achieved a crocetin titer of 196.52 mg/L in shake flasks and 1735.73 mg/L in 10 L fed-batch fermentation, setting a new microbial production record─16-fold higher than previous reports. This work provides a foundation for efficient, large-scale microbial crocetin production and its industrial application. |
| format | Artículo científico |
| id | pubmed_42023900 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Journal of agricultural and food chemistry |
| record_format | pubmed |
| spellingShingle | Enhanced Expression of CCD2 Enables Highly Efficient Crocetin Production in . Guo, Guiping Chen, Huibin Wang, Hailin Zhang, Guilin Fu, Xiaodan Lee, Man Yuen Leung, Yun-Chung Li, Ningyang Liu, Zhen Yarrowia Carotenoids Vitamin A Fungal Proteins Fermentation Metabolic Engineering Dioxygenases Enhanced Expression of CCD2 Enables Highly Efficient Crocetin Production in . Guo, Guiping Chen, Huibin Wang, Hailin Zhang, Guilin Fu, Xiaodan Lee, Man Yuen Leung, Yun-Chung Li, Ningyang Liu, Zhen Yarrowia Carotenoids Vitamin A Fungal Proteins Fermentation Metabolic Engineering Dioxygenases Crocetin, a bioactive carotenoid derivative from saffron, exhibits antioxidant, anti-inflammatory, and metabolic regulatory properties. Its low natural abundance and limited extraction production hinder large-scale production. Microbial synthesis offers a promising alternative but is constrained by low carotenoid cleavage dioxygenase (CCD2) expression. Here, we engineered a zeaxanthin-producing strain to enhance CCD2 expression via fermentation temperature optimization, SUMOstar fusion, subcellular localization, and precursor supply improvement. The engineered strain achieved a crocetin titer of 196.52 mg/L in shake flasks and 1735.73 mg/L in 10 L fed-batch fermentation, setting a new microbial production record─16-fold higher than previous reports. This work provides a foundation for efficient, large-scale microbial crocetin production and its industrial application. |
| title | Enhanced Expression of CCD2 Enables Highly Efficient Crocetin Production in . |
| topic | Yarrowia Carotenoids Vitamin A Fungal Proteins Fermentation Metabolic Engineering Dioxygenases |
| url | https://pubmed.ncbi.nlm.nih.gov/42023900/ |