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| Main Authors: | , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Journal of agricultural and food chemistry
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/42023900/ |
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Table of Contents:
- Enhanced Expression of CCD2 Enables Highly Efficient Crocetin Production in . Guo, Guiping Chen, Huibin Wang, Hailin Zhang, Guilin Fu, Xiaodan Lee, Man Yuen Leung, Yun-Chung Li, Ningyang Liu, Zhen Yarrowia Carotenoids Vitamin A Fungal Proteins Fermentation Metabolic Engineering Dioxygenases Crocetin, a bioactive carotenoid derivative from saffron, exhibits antioxidant, anti-inflammatory, and metabolic regulatory properties. Its low natural abundance and limited extraction production hinder large-scale production. Microbial synthesis offers a promising alternative but is constrained by low carotenoid cleavage dioxygenase (CCD2) expression. Here, we engineered a zeaxanthin-producing strain to enhance CCD2 expression via fermentation temperature optimization, SUMOstar fusion, subcellular localization, and precursor supply improvement. The engineered strain achieved a crocetin titer of 196.52 mg/L in shake flasks and 1735.73 mg/L in 10 L fed-batch fermentation, setting a new microbial production record─16-fold higher than previous reports. This work provides a foundation for efficient, large-scale microbial crocetin production and its industrial application.