Elucidating the Biosynthetic Pathway of Lactone-Type Spirotetronate Polyketide PA-46101s with Inhibitory Activities against Aquatic Pathogenic Bacteria.
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| Format: | Artículo científico |
| Sprache: | en |
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Journal of agricultural and food chemistry
2026
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| author | Hong, Li-Li Cheng, Yijia Zhang, Wenyu Luo, Jiaming Yang, Fan Zhang, Cui-Xian Lin, Hou-Wen Zhou, Yongjun |
| author_facet | Hong, Li-Li Cheng, Yijia Zhang, Wenyu Luo, Jiaming Yang, Fan Zhang, Cui-Xian Lin, Hou-Wen Zhou, Yongjun Hong, Li-Li Cheng, Yijia Zhang, Wenyu Luo, Jiaming Yang, Fan Zhang, Cui-Xian Lin, Hou-Wen Zhou, Yongjun |
| collection | PubMed - marine biology |
| contents | Elucidating the Biosynthetic Pathway of Lactone-Type Spirotetronate Polyketide PA-46101s with Inhibitory Activities against Aquatic Pathogenic Bacteria. Hong, Li-Li Cheng, Yijia Zhang, Wenyu Luo, Jiaming Yang, Fan Zhang, Cui-Xian Lin, Hou-Wen Zhou, Yongjun Streptomyces Anti-Bacterial Agents Polyketides Lactones Biosynthetic Pathways Bacterial Proteins Multigene Family Molecular Structure The spirotetronate polyketides are a class of microbial antibiotics structurally featuring a macrocycle core embedded with a cyclohexene ring spiro-linked to tetronic acid and/or a decalin ring. Of them, PA-46101s are characterized with an extra lactone moiety in the macrocycle scaffold. Despite the remarkable antibacterial activities, the biosynthetic pathway of PA-46101s has not been investigated. Herein, the PA-46101s biosynthetic gene cluster was identified from a species. Overexpression of a LuxR transcriptional regulator led to around a 15-fold rise of PA-46101s production. Characterization of an intermediate accumulated upon deletion of the Baeyer-Villiger monooxygenase homologous gene E1 revealed the first lactone-formation gene from spirotetronate biosynthetic pathways. Characterization of the metabolites of glycosyltransferase gene mutants allowed us to dissect the tailoring glycosylation orders. The obtained PA-46101s analogs exhibited obvious inhibitory activities against the aquatic pathogenic bacteria and . This study paves the way for the structural diversification of PA-46101s through combinatorial biology. |
| format | Artículo científico |
| id | pubmed_42037158 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Journal of agricultural and food chemistry |
| record_format | pubmed |
| spellingShingle | Elucidating the Biosynthetic Pathway of Lactone-Type Spirotetronate Polyketide PA-46101s with Inhibitory Activities against Aquatic Pathogenic Bacteria. Hong, Li-Li Cheng, Yijia Zhang, Wenyu Luo, Jiaming Yang, Fan Zhang, Cui-Xian Lin, Hou-Wen Zhou, Yongjun Streptomyces Anti-Bacterial Agents Polyketides Lactones Biosynthetic Pathways Bacterial Proteins Multigene Family Molecular Structure Elucidating the Biosynthetic Pathway of Lactone-Type Spirotetronate Polyketide PA-46101s with Inhibitory Activities against Aquatic Pathogenic Bacteria. Hong, Li-Li Cheng, Yijia Zhang, Wenyu Luo, Jiaming Yang, Fan Zhang, Cui-Xian Lin, Hou-Wen Zhou, Yongjun Streptomyces Anti-Bacterial Agents Polyketides Lactones Biosynthetic Pathways Bacterial Proteins Multigene Family Molecular Structure The spirotetronate polyketides are a class of microbial antibiotics structurally featuring a macrocycle core embedded with a cyclohexene ring spiro-linked to tetronic acid and/or a decalin ring. Of them, PA-46101s are characterized with an extra lactone moiety in the macrocycle scaffold. Despite the remarkable antibacterial activities, the biosynthetic pathway of PA-46101s has not been investigated. Herein, the PA-46101s biosynthetic gene cluster was identified from a species. Overexpression of a LuxR transcriptional regulator led to around a 15-fold rise of PA-46101s production. Characterization of an intermediate accumulated upon deletion of the Baeyer-Villiger monooxygenase homologous gene E1 revealed the first lactone-formation gene from spirotetronate biosynthetic pathways. Characterization of the metabolites of glycosyltransferase gene mutants allowed us to dissect the tailoring glycosylation orders. The obtained PA-46101s analogs exhibited obvious inhibitory activities against the aquatic pathogenic bacteria and . This study paves the way for the structural diversification of PA-46101s through combinatorial biology. |
| title | Elucidating the Biosynthetic Pathway of Lactone-Type Spirotetronate Polyketide PA-46101s with Inhibitory Activities against Aquatic Pathogenic Bacteria. |
| topic | Streptomyces Anti-Bacterial Agents Polyketides Lactones Biosynthetic Pathways Bacterial Proteins Multigene Family Molecular Structure |
| url | https://pubmed.ncbi.nlm.nih.gov/42037158/ |