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| Auteurs principaux: | , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
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Scientific data
2026
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| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/42082520/ |
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| _version_ | 1868266051134816258 |
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| author | Cai, Yinfan Lu, Jingru Dou, Kangjie Li, Xiaonan Ji, Liling Orozco-terWengel, Pablo Hu, Tingli Zhang, Baowei |
| author_facet | Cai, Yinfan Lu, Jingru Dou, Kangjie Li, Xiaonan Ji, Liling Orozco-terWengel, Pablo Hu, Tingli Zhang, Baowei Cai, Yinfan Lu, Jingru Dou, Kangjie Li, Xiaonan Ji, Liling Orozco-terWengel, Pablo Hu, Tingli Zhang, Baowei |
| collection | PubMed - marine biology |
| contents | A chromosome-level genome assembly of the Tristram's Bunting (Emberiza tristrami). Cai, Yinfan Lu, Jingru Dou, Kangjie Li, Xiaonan Ji, Liling Orozco-terWengel, Pablo Hu, Tingli Zhang, Baowei Emberiza tristrami is a migratory passerine within the genus Emberiza, a taxonomic group that has undergone rapid diversification despite broadly conserved avian karyotypes. Here, we present the first high-quality, chromosome-level reference genome for E. tristrami, generated using HiFi long reads combined with Hi-C sequencing. The final assembly spans 1.33 Gb, with a contig N50 of 22.42 Mb and a scaffold N50 of 64.94 Mb. Hi-C scaffolding anchored 1.23 Gb of assembled sequences into 40 pseudochromosomes, consistent with the known karyotype of the species. We annotated 17,401 protein-coding genes and identified 427.25 Mb of repetitive sequences, accounting for 32.09% of the genome. Genome completeness was assessed using BUSCO, recovering 99.3% of expected single-copy orthologs in genome mode and 91.7% in gene annotation mode, indicating a highly complete genome assembly and a high-quality set of annotated gene models. This chromosome-level genome provides a foundational resource for comparative analyses within Emberiza and other passerines, enabling investigations into chromosome evolution, repeat dynamics, and lineage-specific genomic features associated with rapid species radiation and ecological differentiation. |
| format | Artículo científico |
| id | pubmed_42082520 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Scientific data |
| record_format | pubmed |
| spellingShingle | A chromosome-level genome assembly of the Tristram's Bunting (Emberiza tristrami). Cai, Yinfan Lu, Jingru Dou, Kangjie Li, Xiaonan Ji, Liling Orozco-terWengel, Pablo Hu, Tingli Zhang, Baowei A chromosome-level genome assembly of the Tristram's Bunting (Emberiza tristrami). Cai, Yinfan Lu, Jingru Dou, Kangjie Li, Xiaonan Ji, Liling Orozco-terWengel, Pablo Hu, Tingli Zhang, Baowei Emberiza tristrami is a migratory passerine within the genus Emberiza, a taxonomic group that has undergone rapid diversification despite broadly conserved avian karyotypes. Here, we present the first high-quality, chromosome-level reference genome for E. tristrami, generated using HiFi long reads combined with Hi-C sequencing. The final assembly spans 1.33 Gb, with a contig N50 of 22.42 Mb and a scaffold N50 of 64.94 Mb. Hi-C scaffolding anchored 1.23 Gb of assembled sequences into 40 pseudochromosomes, consistent with the known karyotype of the species. We annotated 17,401 protein-coding genes and identified 427.25 Mb of repetitive sequences, accounting for 32.09% of the genome. Genome completeness was assessed using BUSCO, recovering 99.3% of expected single-copy orthologs in genome mode and 91.7% in gene annotation mode, indicating a highly complete genome assembly and a high-quality set of annotated gene models. This chromosome-level genome provides a foundational resource for comparative analyses within Emberiza and other passerines, enabling investigations into chromosome evolution, repeat dynamics, and lineage-specific genomic features associated with rapid species radiation and ecological differentiation. |
| title | A chromosome-level genome assembly of the Tristram's Bunting (Emberiza tristrami). |
| url | https://pubmed.ncbi.nlm.nih.gov/42082520/ |