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| Autores principales: | , , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
International journal of biological macromolecules
2026
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| Materias: | |
| Acceso en línea: | https://pubmed.ncbi.nlm.nih.gov/42086136/ |
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- An injectable pH-responsive marine polysaccharide hydrogel (AE&LF@pOA) for sequential therapy of infected diabetic wounds. Zhao, Meiyue Wu, Jialing Geng, Lihua Wu, Ning Yue, Yang Zhang, Quanbin Liu, Huaide Wang, Jing Hydrogels Animals Polysaccharides Hydrogen-Ion Concentration Wound Healing Alginates Anti-Bacterial Agents Staphylococcus aureus Polylysine Rats Wound Infection Male Sepharose Injections Drug Liberation Diabetes Mellitus, Experimental Humans Antioxidants Chronic diabetic wound healing remains challenging owing to impaired angiogenesis, persistent inflammation, and a high risk of infection. To address these limitations, we developed an injectable, pH-responsive hydrogel based on dynamic crosslinking between phenylboronic acid-grafted oxidized agarose (pOA) and alginate oligosaccharide-conjugated ε-polylysine (AE). This hydrogel exhibits antibacterial and antioxidant properties, enabling on-demand drug release triggered by the acidic microenvironment of chronic wounds. To enhance therapeutic efficacy, low-molecular-weight fucoidan (LF), a potent pro-angiogenic marine polysaccharide, was encapsulated within the hydrogel. Physicochemical characterizations confirmed that the hydrogel possesses excellent structural stability, injectability, and pH-responsive sustained release properties for LF and AE. Subsequently, In vitro tests showed that the AE&LF@pOA hydrogel effectively controlled infection with a 77.3% antibacterial rate against Staphylococcus aureus and showed ROS scavenging activity. It also regulated the inflammatory response, reducing pro-inflammatory cytokines IL-1β and IL-6 by 53.8% and 64.4%, respectively while increasing anti-inflammatory cytokines IL-10 and TGF-β1 by 2.8-fold and 1.0-fold. Moreover, the hydrogel stimulated neovascularization, leading to a 4.7-fold increase in VEGF expression and a 50% increase in CD-31 microvessel density. Animal studies confirmed that the dressing accelerated macroscopic healing. The hydrogel accelerated wound closure to 85.8% by day 7, 2.7-fold higher than controls, with the residual wound area shrinking to just 1.4% by day 14. Histological analysis further demonstrated complete re-epithelialization to 80.3 μm thickness, and mature collagen deposition. Overall, this responsive hydrogel offers a promising strategy for treating chronic diabetic wounds.