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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Plant physiology
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/42093082/ |
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| _version_ | 1868266051121184768 |
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| author | Hu, Jinlu Zhao, Kuo Chen, Yu Ge, Xingwu Yang, Jing Dykes, Gregory F Shi, Jian-Yu Liu, Lu-Ning |
| author_facet | Hu, Jinlu Zhao, Kuo Chen, Yu Ge, Xingwu Yang, Jing Dykes, Gregory F Shi, Jian-Yu Liu, Lu-Ning Hu, Jinlu Zhao, Kuo Chen, Yu Ge, Xingwu Yang, Jing Dykes, Gregory F Shi, Jian-Yu Liu, Lu-Ning |
| collection | PubMed - marine biology |
| contents | Overexpression of β-carboxysomes increases photosynthesis and growth in Synechocystis sp. PCC 6803. Hu, Jinlu Zhao, Kuo Chen, Yu Ge, Xingwu Yang, Jing Dykes, Gregory F Shi, Jian-Yu Liu, Lu-Ning Cyanobacteria are photoautotrophic cell factories capable of converting carbon dioxide into valuable chemicals. The rate-limiting step in photosynthesis is the enzymatic dark reaction catalyzed by ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO). The carboxysome is a protein-based organelle that plays a central role in cyanobacterial carbon fixation. It consists of thousands of subunits, including hexameric and pentameric proteins, that form a shell to encapsulate the enzymes RuBisCO and carbonic anhydrase. Here, we enhanced β-carboxysome biogenesis in Synechocystis sp. PCC 6803 by overexpressing a full or partial set of endogenous β-carboxysome components. Overexpression of carboxysome proteins altered protein stoichiometry, morphology, and cellular abundance of β-carboxysomes, as well as improved photosynthetic activities and cell growth. These findings indicate that targeted carboxysome overexpression enhances photosynthetic efficiency and growth of cyanobacteria under favorable conditions. This study provides a framework for engineering cyanobacterial chassis cells as microbial cell factories and carboxysome-based CO2-concentrating mechanisms in heterologous hosts, including crop plants, to improve photosynthetic productivity for sustainable bioproduction. |
| format | Artículo científico |
| id | pubmed_42093082 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Plant physiology |
| record_format | pubmed |
| spellingShingle | Overexpression of β-carboxysomes increases photosynthesis and growth in Synechocystis sp. PCC 6803. Hu, Jinlu Zhao, Kuo Chen, Yu Ge, Xingwu Yang, Jing Dykes, Gregory F Shi, Jian-Yu Liu, Lu-Ning Overexpression of β-carboxysomes increases photosynthesis and growth in Synechocystis sp. PCC 6803. Hu, Jinlu Zhao, Kuo Chen, Yu Ge, Xingwu Yang, Jing Dykes, Gregory F Shi, Jian-Yu Liu, Lu-Ning Cyanobacteria are photoautotrophic cell factories capable of converting carbon dioxide into valuable chemicals. The rate-limiting step in photosynthesis is the enzymatic dark reaction catalyzed by ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO). The carboxysome is a protein-based organelle that plays a central role in cyanobacterial carbon fixation. It consists of thousands of subunits, including hexameric and pentameric proteins, that form a shell to encapsulate the enzymes RuBisCO and carbonic anhydrase. Here, we enhanced β-carboxysome biogenesis in Synechocystis sp. PCC 6803 by overexpressing a full or partial set of endogenous β-carboxysome components. Overexpression of carboxysome proteins altered protein stoichiometry, morphology, and cellular abundance of β-carboxysomes, as well as improved photosynthetic activities and cell growth. These findings indicate that targeted carboxysome overexpression enhances photosynthetic efficiency and growth of cyanobacteria under favorable conditions. This study provides a framework for engineering cyanobacterial chassis cells as microbial cell factories and carboxysome-based CO2-concentrating mechanisms in heterologous hosts, including crop plants, to improve photosynthetic productivity for sustainable bioproduction. |
| title | Overexpression of β-carboxysomes increases photosynthesis and growth in Synechocystis sp. PCC 6803. |
| url | https://pubmed.ncbi.nlm.nih.gov/42093082/ |