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Main Authors: Hu, Yuanri, Yan, Xu, Gao, Fengtao, Xu, Dan, Yang, Yingming, Cheng, Jiayu, Chen, Songlin, Cui, Zhongkai
Format: Artículo científico
Language:en
Published: Current research in microbial sciences 2026
Online Access:https://pubmed.ncbi.nlm.nih.gov/42100351/
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author Hu, Yuanri
Yan, Xu
Gao, Fengtao
Xu, Dan
Yang, Yingming
Cheng, Jiayu
Chen, Songlin
Cui, Zhongkai
author_facet Hu, Yuanri
Yan, Xu
Gao, Fengtao
Xu, Dan
Yang, Yingming
Cheng, Jiayu
Chen, Songlin
Cui, Zhongkai
Hu, Yuanri
Yan, Xu
Gao, Fengtao
Xu, Dan
Yang, Yingming
Cheng, Jiayu
Chen, Songlin
Cui, Zhongkai
collection PubMed - marine biology
contents Probiotic-driven microbiome remodeling is associated with coordinated immune and metabolic responses, improving growth and disease resistance in farmed tongue sole (). Hu, Yuanri Yan, Xu Gao, Fengtao Xu, Dan Yang, Yingming Cheng, Jiayu Chen, Songlin Cui, Zhongkai In flatfish aquaculture, labour-intensive tank cleaning represents a major operational challenge, limiting sustainability due to its high labour requirements and associated costs. We tested a new semi-closed recirculating aquaculture system (RAS) protocol for (tongue sole), replacing manual cleaning with post-feeding water exchange (80% drained) and probiotic application. Compared with control groups, the probiotic-water exchange protocol significantly improved growth (+0.18%/day) and survival (+7.9%), while shifting the gut microbiota from a -dominated configuration to a -dominated one. Metagenomics revealed that became the predominant taxon (86%) in the probiotic group, accompanied by the enrichment of quorum sensing pathways, CAZymes (CEs, AAs), and nutrient metabolism functions. Histological examination showed improvements in the intestinal muscular layer and villi structure. Multi-tissue transcriptomics identified systemic changes in immune and metabolic pathways, including activation of intestinal immune networks (IgA production, NF-κB signaling) and antimicrobial peptide genes. Liver, gill, and skin transcriptomes revealed enhanced DNA repair, cytokine signaling, and barrier pathways. JAK-STAT pathway was also activated, linking microbial metabolite sensing to growth promotion (). The probiotic-integrated protocol modifies the gut microbiome by shifting microbial composition through changes in competitive interactions and microbial signaling pathways. It also improves the intestinal wall, overall immunity, and nutrient absorption. These findings provide insights into the microbiome-host interaction under probiotic treatment and suggest that this strategy may offer potential benefits under farm conditions, but further studies are needed to validate its safety and ecological implications.
format Artículo científico
id pubmed_42100351
institution PubMed
language en
publishDate 2026
publisher Current research in microbial sciences
record_format pubmed
spellingShingle Probiotic-driven microbiome remodeling is associated with coordinated immune and metabolic responses, improving growth and disease resistance in farmed tongue sole ().
Hu, Yuanri
Yan, Xu
Gao, Fengtao
Xu, Dan
Yang, Yingming
Cheng, Jiayu
Chen, Songlin
Cui, Zhongkai
Probiotic-driven microbiome remodeling is associated with coordinated immune and metabolic responses, improving growth and disease resistance in farmed tongue sole (). Hu, Yuanri Yan, Xu Gao, Fengtao Xu, Dan Yang, Yingming Cheng, Jiayu Chen, Songlin Cui, Zhongkai In flatfish aquaculture, labour-intensive tank cleaning represents a major operational challenge, limiting sustainability due to its high labour requirements and associated costs. We tested a new semi-closed recirculating aquaculture system (RAS) protocol for (tongue sole), replacing manual cleaning with post-feeding water exchange (80% drained) and probiotic application. Compared with control groups, the probiotic-water exchange protocol significantly improved growth (+0.18%/day) and survival (+7.9%), while shifting the gut microbiota from a -dominated configuration to a -dominated one. Metagenomics revealed that became the predominant taxon (86%) in the probiotic group, accompanied by the enrichment of quorum sensing pathways, CAZymes (CEs, AAs), and nutrient metabolism functions. Histological examination showed improvements in the intestinal muscular layer and villi structure. Multi-tissue transcriptomics identified systemic changes in immune and metabolic pathways, including activation of intestinal immune networks (IgA production, NF-κB signaling) and antimicrobial peptide genes. Liver, gill, and skin transcriptomes revealed enhanced DNA repair, cytokine signaling, and barrier pathways. JAK-STAT pathway was also activated, linking microbial metabolite sensing to growth promotion (). The probiotic-integrated protocol modifies the gut microbiome by shifting microbial composition through changes in competitive interactions and microbial signaling pathways. It also improves the intestinal wall, overall immunity, and nutrient absorption. These findings provide insights into the microbiome-host interaction under probiotic treatment and suggest that this strategy may offer potential benefits under farm conditions, but further studies are needed to validate its safety and ecological implications.
title Probiotic-driven microbiome remodeling is associated with coordinated immune and metabolic responses, improving growth and disease resistance in farmed tongue sole ().
url https://pubmed.ncbi.nlm.nih.gov/42100351/