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| Main Authors: | , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Fish & shellfish immunology
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/42105828/ |
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Table of Contents:
- Functional characterization of STING in large yellow croaker (Larimichthys crocea), a pivotal regulator associated with TRIF, TRAF3, IRF3, and IRF7 in modulating antiviral signaling. Li, Yi Lin Zhang, Jia Xi Tang, Jun Chun Li, Ying Zhu, Xuan Han, Jian Zhong Jiang, Yong Hua Wang, Yi Lei Zou, Peng Fei Animals Fish Proteins Immunity, Innate Fish Diseases Perciformes Gene Expression Regulation Membrane Proteins Signal Transduction Amino Acid Sequence Sequence Alignment Pseudomonas cGAS-STING Signaling Pathway Poly I-C Phylogeny Interferon Regulatory Factor-3 Lipopolysaccharides Gene Expression Profiling STING Protein TNF Receptor-Associated Factor 3 Interferon Regulatory Factor-7 Base Sequence Adaptor Proteins, Vesicular Transport STING serves as a crucial adaptor protein in cytosolic nucleic acid sensing pathways that initiate host antiviral defense mechanisms. Herein, we report the molecular cloning and functional characterization of a STING ortholog namely Lc-STING in large yellow croaker (Larimichthys crocea). The complete coding sequence of Lc-STING comprises 1227-bp, encoding a 408-aa protein containing a TMEM173 domain and three transmembrane regions. Lc-STING exhibits ubiquitous tissue distribution, with maximal levels in the intestine and minimal in the brain. Upon challenge with poly I:C, LPS, PGN, or Pseudomonas plecoglossicida, Lc-STING expression was markedly elevated in immune-related tissues. As a cytoplasmic distribution protein, Lc-STING overexpression dose-dependently activated NF-κB, IRF3, IRF7, and type I IFN promoters. Notably, the co-expression of Lc-STING with Lc-TRIF, Lc-TRAF3, Lc-IRF3, or Lc-IRF7 substantially influenced antiviral signaling cascade, attenuating NF-κB while amplifying IRF3, IRF7, and type I IFN promoter activation. Such combinatorial expressions also significantly impacted the transcription of antiviral and inflammatory genes, encompassing IL-1β, IRF3, TNF-α, IRF7, ISG15, Mx, and ISG56. These findings collectively establish Lc-STING as a pivotal mediator of antiviral immunity in large yellow croaker, engaging in intricate crosstalk with multiple signaling components to coordinate balanced activation of interferon regulatory pathways while tempering inflammatory NF-κB responses.