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| Format: | Artículo científico |
| Sprache: | en |
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Fish & shellfish immunology
2026
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| Schlagworte: | |
| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/42114400/ |
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Inhaltsangabe:
- Optimization of lyophilization medium for Vibrio harveyi phage and application in oral delivery to groupers. Kang, Shaozhu Wu, Siting Qin, Qiwei Wei, Jingguang Animals Vibrio Freeze Drying Bacteriophages Vibrio Infections Fish Diseases Animal Feed Bass Phage Therapy Diet Immunonutrition Diet Administration, Oral Vibrio harveyi is a major pathogen in grouper aquaculture, and the rise of antibiotic resistance necessitates the development of new control strategies. Phage therapy is considered a promising alternative, but formulation stability limits its practical application. This study aimed to optimize the lyoprotectant formulation for the V. harveyi phage V-YDF132 and to evaluate its immunomodulatory effects as a feed additive in grouper, as well as its prophylactic efficacy against V. harveyi infection. Among nine candidate composite protectants screened, formula P9 (consisting of inulin, skim milk, gelatin, and sodium L-glutamate) was identified as the optimal formulation. The resulting preparation exhibited good stability: the phage titer decreased by 0.51 log immediately after lyophilization; after 21 d at 37 °C in an accelerated stability test, the titer decreased by only 0.90 log; and after 9 months at 4 °C, the titer decreased by 0.51 log. During a 14-d feeding trial, there were no differences in growth performance between the phage-fed and control groups. Feeding phage-supplemented diets significantly increased the activities of serum alkaline phosphatase (AKP), nitric oxide synthase (NOS), and lysozyme (LYZ), and significantly upregulated the expression of immune-related genes (CD4, CD8, IgM, and TCRβ) and inflammatory cytokines (IL-6 and IL-8) in the spleen. Challenge tests showed that the phage-fed group achieved a relative percent survival (RPS) of 28.6%. In summary, the lyophilized phage V-YDF132 formulation can serve as a potential immunomodulator and, when administered orally, enhances grouper resistance to V. harveyi.