Phenotypic and whole-genome characterization of a novel Lysinibacillus fusiformis PWR01 isolated from rubber latex with multifunctional probiotic and antimicrobial potential.
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| Natura: | Artículo científico |
| Lingua: | en |
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Microbial pathogenesis
2026
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| author | Permpoonpattana, Patima Chakma, Ashim Mwamburi, Samuel Mwakisha Khang, Luu Tang Phuc Sihamok, Waraphorn Phetduang, Kritsada Therdtatha, Phatthanaphong Islam, Sk Injamamul Amin, Nusrat Shahed, Khandker Sangsawad, Papungkorn Linh, Nguyen Vu |
| author_facet | Permpoonpattana, Patima Chakma, Ashim Mwamburi, Samuel Mwakisha Khang, Luu Tang Phuc Sihamok, Waraphorn Phetduang, Kritsada Therdtatha, Phatthanaphong Islam, Sk Injamamul Amin, Nusrat Shahed, Khandker Sangsawad, Papungkorn Linh, Nguyen Vu Permpoonpattana, Patima Chakma, Ashim Mwamburi, Samuel Mwakisha Khang, Luu Tang Phuc Sihamok, Waraphorn Phetduang, Kritsada Therdtatha, Phatthanaphong Islam, Sk Injamamul Amin, Nusrat Shahed, Khandker Sangsawad, Papungkorn Linh, Nguyen Vu |
| collection | PubMed - marine biology |
| contents | Phenotypic and whole-genome characterization of a novel Lysinibacillus fusiformis PWR01 isolated from rubber latex with multifunctional probiotic and antimicrobial potential. Permpoonpattana, Patima Chakma, Ashim Mwamburi, Samuel Mwakisha Khang, Luu Tang Phuc Sihamok, Waraphorn Phetduang, Kritsada Therdtatha, Phatthanaphong Islam, Sk Injamamul Amin, Nusrat Shahed, Khandker Sangsawad, Papungkorn Linh, Nguyen Vu Genome, Bacterial Bacillaceae Probiotics Whole Genome Sequencing Phenotype Latex Hydrogen-Ion Concentration Biofilms Phylogeny Antioxidants Pseudomonas aeruginosa Anti-Bacterial Agents Anti-Infective Agents Temperature Spores, Bacterial Microbial Sensitivity Tests Microbial resources from plant-derived environments harbor a reservoir of functionally diverse probiotic candidates, yet information on the probiotic potential of Lysinibacillus species from rubber latex environment remains limited. This study aimed to characterize Lysinibacillus sp. PWR01, isolated from rubber latex, through comprehensive integrated phenotypic, functional, and genomic analyses to evaluate its biotechnological potential. Morphological, biochemical, and physiological assays were combined with whole-genome sequencing, comparative genomics, and genome functional annotation. PWR01 demonstrated strong tolerance to acidic pH and bile salt conditions, high sporulation efficiency, and pronounced antibiofilm activity against Pseudomonas aeruginosa. In addition, the cell-free supernatant inhibited Streptococcus agalactiae and Aeromonas hydrophila in a concentration-dependent manner across a range of temperature and pH conditions. The strain also exhibited notable antioxidant activity and maintained high cell viability during storage. Genome analysis revealed a 5.0 Mb genome with 4909 predicted genes and 97% average nucleotide identity (ANI) with Lysinibacillus fusiformis. Functional annotation identified genes associated with sporulation, stress tolerance, carbohydrate metabolism, and secondary metabolite biosynthesis, including polyketide and nonribosomal peptide biosynthetic pathways. Despite the presence of antimicrobial resistance-associated genes, no corresponding phenotypic antimicrobial resistance was detected. These findings collectively highlight Lysinibacillus fusiformis PWR01 as a metabolically versatile and resilient strain with considerable probiotic and biotechnological potential. |
| format | Artículo científico |
| id | pubmed_42114786 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Microbial pathogenesis |
| record_format | pubmed |
| spellingShingle | Phenotypic and whole-genome characterization of a novel Lysinibacillus fusiformis PWR01 isolated from rubber latex with multifunctional probiotic and antimicrobial potential. Permpoonpattana, Patima Chakma, Ashim Mwamburi, Samuel Mwakisha Khang, Luu Tang Phuc Sihamok, Waraphorn Phetduang, Kritsada Therdtatha, Phatthanaphong Islam, Sk Injamamul Amin, Nusrat Shahed, Khandker Sangsawad, Papungkorn Linh, Nguyen Vu Genome, Bacterial Bacillaceae Probiotics Whole Genome Sequencing Phenotype Latex Hydrogen-Ion Concentration Biofilms Phylogeny Antioxidants Pseudomonas aeruginosa Anti-Bacterial Agents Anti-Infective Agents Temperature Spores, Bacterial Microbial Sensitivity Tests Phenotypic and whole-genome characterization of a novel Lysinibacillus fusiformis PWR01 isolated from rubber latex with multifunctional probiotic and antimicrobial potential. Permpoonpattana, Patima Chakma, Ashim Mwamburi, Samuel Mwakisha Khang, Luu Tang Phuc Sihamok, Waraphorn Phetduang, Kritsada Therdtatha, Phatthanaphong Islam, Sk Injamamul Amin, Nusrat Shahed, Khandker Sangsawad, Papungkorn Linh, Nguyen Vu Genome, Bacterial Bacillaceae Probiotics Whole Genome Sequencing Phenotype Latex Hydrogen-Ion Concentration Biofilms Phylogeny Antioxidants Pseudomonas aeruginosa Anti-Bacterial Agents Anti-Infective Agents Temperature Spores, Bacterial Microbial Sensitivity Tests Microbial resources from plant-derived environments harbor a reservoir of functionally diverse probiotic candidates, yet information on the probiotic potential of Lysinibacillus species from rubber latex environment remains limited. This study aimed to characterize Lysinibacillus sp. PWR01, isolated from rubber latex, through comprehensive integrated phenotypic, functional, and genomic analyses to evaluate its biotechnological potential. Morphological, biochemical, and physiological assays were combined with whole-genome sequencing, comparative genomics, and genome functional annotation. PWR01 demonstrated strong tolerance to acidic pH and bile salt conditions, high sporulation efficiency, and pronounced antibiofilm activity against Pseudomonas aeruginosa. In addition, the cell-free supernatant inhibited Streptococcus agalactiae and Aeromonas hydrophila in a concentration-dependent manner across a range of temperature and pH conditions. The strain also exhibited notable antioxidant activity and maintained high cell viability during storage. Genome analysis revealed a 5.0 Mb genome with 4909 predicted genes and 97% average nucleotide identity (ANI) with Lysinibacillus fusiformis. Functional annotation identified genes associated with sporulation, stress tolerance, carbohydrate metabolism, and secondary metabolite biosynthesis, including polyketide and nonribosomal peptide biosynthetic pathways. Despite the presence of antimicrobial resistance-associated genes, no corresponding phenotypic antimicrobial resistance was detected. These findings collectively highlight Lysinibacillus fusiformis PWR01 as a metabolically versatile and resilient strain with considerable probiotic and biotechnological potential. |
| title | Phenotypic and whole-genome characterization of a novel Lysinibacillus fusiformis PWR01 isolated from rubber latex with multifunctional probiotic and antimicrobial potential. |
| topic | Genome, Bacterial Bacillaceae Probiotics Whole Genome Sequencing Phenotype Latex Hydrogen-Ion Concentration Biofilms Phylogeny Antioxidants Pseudomonas aeruginosa Anti-Bacterial Agents Anti-Infective Agents Temperature Spores, Bacterial Microbial Sensitivity Tests |
| url | https://pubmed.ncbi.nlm.nih.gov/42114786/ |