Yeast Display and Competitive FACS: Powerful Tools for the Selection of Rare Clones from an scFv Library.
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| Format: | Artículo científico |
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Methods in molecular biology (Clifton, N.J.)
2026
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| _version_ | 1868266048926515200 |
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| author | Sun, Yue Ban, Bhupal Blake, Diane A |
| author_facet | Sun, Yue Ban, Bhupal Blake, Diane A Sun, Yue Ban, Bhupal Blake, Diane A |
| collection | PubMed - marine biology |
| contents | Yeast Display and Competitive FACS: Powerful Tools for the Selection of Rare Clones from an scFv Library. Sun, Yue Ban, Bhupal Blake, Diane A Single-Chain Antibodies Peptide Library Flow Cytometry Saccharomyces cerevisiae Cell Surface Display Techniques Recombinant Proteins Humans Haptens Recombinant antibodies and novel display technologies have significantly advanced the development of antibodies. However, new techniques are still required to select rare hapten-specific clones from large recombinant libraries. In this chapter, we describe a novel selection procedure for the identification and subsequent isolation of rare, recombinant antibodies from a relatively large immune library (∼ 4.4 × 10). This technique, which combined preselection of an immune phage library with yeast display and competitive FACS, yielded high percentages (20%-40%) of hapten-specific scFvs in the final pool of selected cells. |
| format | Artículo científico |
| id | pubmed_42129091 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Methods in molecular biology (Clifton, N.J.) |
| record_format | pubmed |
| spellingShingle | Yeast Display and Competitive FACS: Powerful Tools for the Selection of Rare Clones from an scFv Library. Sun, Yue Ban, Bhupal Blake, Diane A Single-Chain Antibodies Peptide Library Flow Cytometry Saccharomyces cerevisiae Cell Surface Display Techniques Recombinant Proteins Humans Haptens Yeast Display and Competitive FACS: Powerful Tools for the Selection of Rare Clones from an scFv Library. Sun, Yue Ban, Bhupal Blake, Diane A Single-Chain Antibodies Peptide Library Flow Cytometry Saccharomyces cerevisiae Cell Surface Display Techniques Recombinant Proteins Humans Haptens Recombinant antibodies and novel display technologies have significantly advanced the development of antibodies. However, new techniques are still required to select rare hapten-specific clones from large recombinant libraries. In this chapter, we describe a novel selection procedure for the identification and subsequent isolation of rare, recombinant antibodies from a relatively large immune library (∼ 4.4 × 10). This technique, which combined preselection of an immune phage library with yeast display and competitive FACS, yielded high percentages (20%-40%) of hapten-specific scFvs in the final pool of selected cells. |
| title | Yeast Display and Competitive FACS: Powerful Tools for the Selection of Rare Clones from an scFv Library. |
| topic | Single-Chain Antibodies Peptide Library Flow Cytometry Saccharomyces cerevisiae Cell Surface Display Techniques Recombinant Proteins Humans Haptens |
| url | https://pubmed.ncbi.nlm.nih.gov/42129091/ |