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| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
International journal of biological macromolecules
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/42140302/ |
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Table of Contents:
- Ultrasound-assisted valorization of Posidonia oceanica polysaccharides: Discovery of novel monosaccharides and enhanced multifunctional bioactivity. Malaoui, Karima Ben Haj Mbarek, Asma Karouche, Saida Itaimi Dammak, Mariem Majdoub, Hatem Le Cerf, Didier Bendif, Hamdi Taha, Tarek H Elfalleh, Walid Ali, Mohamed A M Yildiz, Ilyas Bouslama, Lamjed Bensouici, Chawki Laichi, Yacine Garzoli, Stefania Laouani, Aicha Polysaccharides Alismatales Monosaccharides Plant Leaves Antioxidants Antiviral Agents Humans Ultrasonic Waves Molecular Weight Herpesvirus 2, Human Rhizome Posidonia oceanica, a Mediterranean seagrass, represents a promising marine resource for the production of high-value polysaccharides with potential industrial and biomedical applications. In this study, ultrasound-assisted extraction (UAE) was deliberately applied under non-optimized conditions to investigate its impact on the structure of polysaccharides obtained from P. oceanica leaves (MKF) and rhizomes (MKR), yielding 0.63% and 0.43% (w/w), respectively. GC-MS analysis of MKF revealed a diverse monosaccharide profile, including xylose (22.7%), arabinose (21.7%), glucose (16.3%), rhamnose (13.2%), and, for the first time in Posidonia oceanica, tentatively identified talose (7.2%), lyxose (7.7%), and fucose (4.0%). Molecular weight determination via size-exclusion Chromatography showed 394,000 g·mol for MKF and 180,000 g·mol for MKR. Leaf-derived polysaccharides exhibited significantly stronger antioxidant activity (DPPH IC₅₀ = 10.91 ± 0.70 μg/mL; ABTS IC₅₀ = 6.78 ± 0.07 μg/mL) compared to rhizome extracts. Both extracts demonstrated high photoprotective potential (SPF = 42.3 and 39.3 for MKF and MKR, respectively) and substantial antiviral potency toward type 2 herpes simplex virus, with selectivity indices of 750-850 and no detectable cytotoxicity up to 2500 μg/mL. These results suggest that ultrasound-induced structural modifications enhance the bioactivity of P. oceanica polysaccharides, positioning them as multifunctional biomolecules with potential applications in pharmaceuticals, cosmeceuticals, and other industrial sectors.