Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
2026
|
| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/42140403/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266048911835136 |
|---|---|
| author | Hu, Chun Li, Ziyin Wu, Guanglin Lee, Jae-Seong Miao, Jingjing Dong, Yunwei Maszczyk, Piotr Sharifinia, Moslem Singh, Nisha Shang, Yueyong Wang, Youji |
| author_facet | Hu, Chun Li, Ziyin Wu, Guanglin Lee, Jae-Seong Miao, Jingjing Dong, Yunwei Maszczyk, Piotr Sharifinia, Moslem Singh, Nisha Shang, Yueyong Wang, Youji Hu, Chun Li, Ziyin Wu, Guanglin Lee, Jae-Seong Miao, Jingjing Dong, Yunwei Maszczyk, Piotr Sharifinia, Moslem Singh, Nisha Shang, Yueyong Wang, Youji |
| collection | PubMed - marine biology |
| contents | Combined exposure to nano-TiO₂ and carbamazepine exacerbates reproductive toxicity in mussels Mytilus coruscus by disrupting steroid hormone homeostasis. Hu, Chun Li, Ziyin Wu, Guanglin Lee, Jae-Seong Miao, Jingjing Dong, Yunwei Maszczyk, Piotr Sharifinia, Moslem Singh, Nisha Shang, Yueyong Wang, Youji Animals Titanium Carbamazepine Water Pollutants, Chemical Reproduction Mytilus Male Gonadal Steroid Hormones Homeostasis Female Gonads Metal Nanoparticles Endocrine Disruptors Titanium dioxide nanoparticles (nano-TiO₂) and carbamazepine (CBZ) are both emerging contaminants of concern in coastal environments. Current monitoring data indicate that nano-TiO₂ occur in nearshore seawater affected by land-based inputs, and CBZ is persistently detected in estuarine and nearshore waters; therefore, these contaminants may co-occur at a regional scale. In this study, the thick-shelled mussel (Mytilus coruscus) was used as a model organism. Nominal concentrations of nano-TiO₂ (100 μg/L) and CBZ (10 μg/L) were applied in single and combined exposure experiments over 14 days. Particle characterization, gonadal morphology, sex steroid hormone levels, and key gene expression analyses were integrated to evaluate reproductive hormone-disrupting effects. The results showed that nano-TiO₂ readily aggregated in seawater, and distinct nano-TiO₂ aggregates were observed on the surface of CBZ crystals in dried mixed samples. Phenotypically, exposure to either pollutant alone reduced the gonadal area and gonadosomatic index, showing significant sex-specific differences in the CBZ group. In contrast, combined exposure caused more pronounced reproductive tissue damage. Hormonally, exposure disrupted sex steroid homeostasis, decreasing progesterone, estrone, and estradiol (E2), accompanied by increased testosterone (T). These hormonal changes exhibited sex-dependent patterns across treatments, with a decrease in E2 and an increase in T being more pronounced in the combined-exposure group. Molecularly, exposures significantly downregulated SF-1 and Wnt signaling genes (β-catenin, Wnt4), while upregulating Wnt7b. Overall, single exposure to nominal concentrations of nano-TiO₂ or CBZ was sufficient to induce sex-specific reproductive damage in M. coruscus, whereas combined exposure caused more pronounced changes at the morphological, hormonal, and transcriptional levels. |
| format | Artículo científico |
| id | pubmed_42140403 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Comparative biochemistry and physiology. Toxicology & pharmacology : CBP |
| record_format | pubmed |
| spellingShingle | Combined exposure to nano-TiO₂ and carbamazepine exacerbates reproductive toxicity in mussels Mytilus coruscus by disrupting steroid hormone homeostasis. Hu, Chun Li, Ziyin Wu, Guanglin Lee, Jae-Seong Miao, Jingjing Dong, Yunwei Maszczyk, Piotr Sharifinia, Moslem Singh, Nisha Shang, Yueyong Wang, Youji Animals Titanium Carbamazepine Water Pollutants, Chemical Reproduction Mytilus Male Gonadal Steroid Hormones Homeostasis Female Gonads Metal Nanoparticles Endocrine Disruptors Combined exposure to nano-TiO₂ and carbamazepine exacerbates reproductive toxicity in mussels Mytilus coruscus by disrupting steroid hormone homeostasis. Hu, Chun Li, Ziyin Wu, Guanglin Lee, Jae-Seong Miao, Jingjing Dong, Yunwei Maszczyk, Piotr Sharifinia, Moslem Singh, Nisha Shang, Yueyong Wang, Youji Animals Titanium Carbamazepine Water Pollutants, Chemical Reproduction Mytilus Male Gonadal Steroid Hormones Homeostasis Female Gonads Metal Nanoparticles Endocrine Disruptors Titanium dioxide nanoparticles (nano-TiO₂) and carbamazepine (CBZ) are both emerging contaminants of concern in coastal environments. Current monitoring data indicate that nano-TiO₂ occur in nearshore seawater affected by land-based inputs, and CBZ is persistently detected in estuarine and nearshore waters; therefore, these contaminants may co-occur at a regional scale. In this study, the thick-shelled mussel (Mytilus coruscus) was used as a model organism. Nominal concentrations of nano-TiO₂ (100 μg/L) and CBZ (10 μg/L) were applied in single and combined exposure experiments over 14 days. Particle characterization, gonadal morphology, sex steroid hormone levels, and key gene expression analyses were integrated to evaluate reproductive hormone-disrupting effects. The results showed that nano-TiO₂ readily aggregated in seawater, and distinct nano-TiO₂ aggregates were observed on the surface of CBZ crystals in dried mixed samples. Phenotypically, exposure to either pollutant alone reduced the gonadal area and gonadosomatic index, showing significant sex-specific differences in the CBZ group. In contrast, combined exposure caused more pronounced reproductive tissue damage. Hormonally, exposure disrupted sex steroid homeostasis, decreasing progesterone, estrone, and estradiol (E2), accompanied by increased testosterone (T). These hormonal changes exhibited sex-dependent patterns across treatments, with a decrease in E2 and an increase in T being more pronounced in the combined-exposure group. Molecularly, exposures significantly downregulated SF-1 and Wnt signaling genes (β-catenin, Wnt4), while upregulating Wnt7b. Overall, single exposure to nominal concentrations of nano-TiO₂ or CBZ was sufficient to induce sex-specific reproductive damage in M. coruscus, whereas combined exposure caused more pronounced changes at the morphological, hormonal, and transcriptional levels. |
| title | Combined exposure to nano-TiO₂ and carbamazepine exacerbates reproductive toxicity in mussels Mytilus coruscus by disrupting steroid hormone homeostasis. |
| topic | Animals Titanium Carbamazepine Water Pollutants, Chemical Reproduction Mytilus Male Gonadal Steroid Hormones Homeostasis Female Gonads Metal Nanoparticles Endocrine Disruptors |
| url | https://pubmed.ncbi.nlm.nih.gov/42140403/ |