Serotypic and Genomic Diversity of Vibrio anguillarum in Rainbow Trout Farms in Turkey: Implications for Vibriosis Control and Vaccine Candidate Selection.
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| Auteurs principaux: | , , , , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
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Journal of fish diseases
2026
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| _version_ | 1868266048909737985 |
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| author | Altun, Soner Duman, Muhammed Romalde, Jesús L Ajmi, Nihed Tasci, Gorkem Ozakin, Cuneyt Tuzemen, Nazmiye Ulku Akça, Bekir Ergün, Sebahattin Yılmaz, Sevdan Saticioglu, Izzet Burcin |
| author_facet | Altun, Soner Duman, Muhammed Romalde, Jesús L Ajmi, Nihed Tasci, Gorkem Ozakin, Cuneyt Tuzemen, Nazmiye Ulku Akça, Bekir Ergün, Sebahattin Yılmaz, Sevdan Saticioglu, Izzet Burcin Altun, Soner Duman, Muhammed Romalde, Jesús L Ajmi, Nihed Tasci, Gorkem Ozakin, Cuneyt Tuzemen, Nazmiye Ulku Akça, Bekir Ergün, Sebahattin Yılmaz, Sevdan Saticioglu, Izzet Burcin |
| collection | PubMed - marine biology |
| contents | Serotypic and Genomic Diversity of Vibrio anguillarum in Rainbow Trout Farms in Turkey: Implications for Vibriosis Control and Vaccine Candidate Selection. Altun, Soner Duman, Muhammed Romalde, Jesús L Ajmi, Nihed Tasci, Gorkem Ozakin, Cuneyt Tuzemen, Nazmiye Ulku Akça, Bekir Ergün, Sebahattin Yılmaz, Sevdan Saticioglu, Izzet Burcin Outbreaks of vibriosis caused by Vibrio anguillarum are a persistent constraint on rainbow trout (Oncorhynchus mykiss) aquaculture. However, information on the population structure of field strains in Turkey has been lacking. Here, we report the first systematic serotypic, proteomic, and genomic characterization of 23 V. anguillarum isolates collected over 10 years from rainbow trout farms located in six major aquaculture regions of Turkey. Serological analyses based on microagglutination, supported by ELISA characterization of hyperimmune sera, identified a clear predominance of serotype O1, whereas isolate V12 exhibited a non-agglutinating, atypical O-antigen profile. Protein profiling (SDS-PAGE) and immunoblotting showed largely conserved whole-cell protein patterns among the isolates, but distinct immunogenic bands at 14, 18, and 40 kDa were detected in isolates V18 and V21. Long-read whole-genome sequencing revealed that most Turkish isolates grouped within the global O1 clade, while V12, V25, and V28 isolates occupied more distant branches. Comparative genomics demonstrated a conserved core virulence gene set (RTX toxins, siderophore and iron-uptake systems, motility and adhesion factors, Type VI secretion system), with strain-dependent variation in accessory loci such as anguibactin and T6SS-I. Experimental infections of rainbow trout demonstrated significant differences in virulence among isolates (p |
| format | Artículo científico |
| id | pubmed_42141499 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Journal of fish diseases |
| record_format | pubmed |
| spellingShingle | Serotypic and Genomic Diversity of Vibrio anguillarum in Rainbow Trout Farms in Turkey: Implications for Vibriosis Control and Vaccine Candidate Selection. Altun, Soner Duman, Muhammed Romalde, Jesús L Ajmi, Nihed Tasci, Gorkem Ozakin, Cuneyt Tuzemen, Nazmiye Ulku Akça, Bekir Ergün, Sebahattin Yılmaz, Sevdan Saticioglu, Izzet Burcin Serotypic and Genomic Diversity of Vibrio anguillarum in Rainbow Trout Farms in Turkey: Implications for Vibriosis Control and Vaccine Candidate Selection. Altun, Soner Duman, Muhammed Romalde, Jesús L Ajmi, Nihed Tasci, Gorkem Ozakin, Cuneyt Tuzemen, Nazmiye Ulku Akça, Bekir Ergün, Sebahattin Yılmaz, Sevdan Saticioglu, Izzet Burcin Outbreaks of vibriosis caused by Vibrio anguillarum are a persistent constraint on rainbow trout (Oncorhynchus mykiss) aquaculture. However, information on the population structure of field strains in Turkey has been lacking. Here, we report the first systematic serotypic, proteomic, and genomic characterization of 23 V. anguillarum isolates collected over 10 years from rainbow trout farms located in six major aquaculture regions of Turkey. Serological analyses based on microagglutination, supported by ELISA characterization of hyperimmune sera, identified a clear predominance of serotype O1, whereas isolate V12 exhibited a non-agglutinating, atypical O-antigen profile. Protein profiling (SDS-PAGE) and immunoblotting showed largely conserved whole-cell protein patterns among the isolates, but distinct immunogenic bands at 14, 18, and 40 kDa were detected in isolates V18 and V21. Long-read whole-genome sequencing revealed that most Turkish isolates grouped within the global O1 clade, while V12, V25, and V28 isolates occupied more distant branches. Comparative genomics demonstrated a conserved core virulence gene set (RTX toxins, siderophore and iron-uptake systems, motility and adhesion factors, Type VI secretion system), with strain-dependent variation in accessory loci such as anguibactin and T6SS-I. Experimental infections of rainbow trout demonstrated significant differences in virulence among isolates (p |
| title | Serotypic and Genomic Diversity of Vibrio anguillarum in Rainbow Trout Farms in Turkey: Implications for Vibriosis Control and Vaccine Candidate Selection. |
| url | https://pubmed.ncbi.nlm.nih.gov/42141499/ |