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| Main Authors: | , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Experimental animals
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/42161565/ |
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| _version_ | 1868266046445584385 |
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| author | Miyaki, Marina Komiya, Yusuke Nakada, Minori Noguchi, Kensei Yokoyama, Issei Suzuki, Takahiro Ojima, Koichi Arihara, Keizo Sasaki, Nobuya |
| author_facet | Miyaki, Marina Komiya, Yusuke Nakada, Minori Noguchi, Kensei Yokoyama, Issei Suzuki, Takahiro Ojima, Koichi Arihara, Keizo Sasaki, Nobuya Miyaki, Marina Komiya, Yusuke Nakada, Minori Noguchi, Kensei Yokoyama, Issei Suzuki, Takahiro Ojima, Koichi Arihara, Keizo Sasaki, Nobuya |
| collection | PubMed - marine biology |
| contents | Tensin2 deficiency transiently accelerates early-phase regenerative myofiber growth following cardiotoxin-induced injury in mice. Miyaki, Marina Komiya, Yusuke Nakada, Minori Noguchi, Kensei Yokoyama, Issei Suzuki, Takahiro Ojima, Koichi Arihara, Keizo Sasaki, Nobuya Tensin2 (TNS2) is a focal adhesion-associated protein that can negatively regulate insulin/insulin-like growth factor 1 (IGF-1) signaling, but its role in skeletal muscle regeneration remains unclear. To address this, we analyzed a TNS2-related immunoreactive signal detected with an anti-phospho-TNS2 (Tyr483) antibody during C2C12 myogenic differentiation and in a cardiotoxin (CTX)-induced tibialis anterior (TA) injury model, and assessed regeneration in Tns2 mutant mice. Under basal conditions, Tns2 mice showed no obvious abnormalities in muscle histology, myofiber size, or phosphorylation of Akt and p70 ribosomal protein S6 kinase (p70S6K), a canonical downstream effector of mTORC1 signaling, compared with wild-type (WT) mice, although the soleus muscle/body weight ratio was slightly increased. In C2C12 cells, the anti-phospho-TNS2 (Tyr483)-reactive signal was stronger during proliferation than during differentiation. In vivo, this signal increased transiently during the early phase of CTX-mediated regeneration. After injury, Tns2 mice showed a greater number of centrally nucleated fibers at day 4, larger myofibers at day 7, and a higher TA muscle/body weight ratio at day 15. In addition, phosphorylation of p70S6K was increased in regenerating muscle in Tns2 mice. These findings suggest that TNS2-related signaling may act as a context-dependent modulator of regenerative myofiber growth, possibly through augmented anabolic signaling during early regeneration. |
| format | Artículo científico |
| id | pubmed_42161565 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Experimental animals |
| record_format | pubmed |
| spellingShingle | Tensin2 deficiency transiently accelerates early-phase regenerative myofiber growth following cardiotoxin-induced injury in mice. Miyaki, Marina Komiya, Yusuke Nakada, Minori Noguchi, Kensei Yokoyama, Issei Suzuki, Takahiro Ojima, Koichi Arihara, Keizo Sasaki, Nobuya Tensin2 deficiency transiently accelerates early-phase regenerative myofiber growth following cardiotoxin-induced injury in mice. Miyaki, Marina Komiya, Yusuke Nakada, Minori Noguchi, Kensei Yokoyama, Issei Suzuki, Takahiro Ojima, Koichi Arihara, Keizo Sasaki, Nobuya Tensin2 (TNS2) is a focal adhesion-associated protein that can negatively regulate insulin/insulin-like growth factor 1 (IGF-1) signaling, but its role in skeletal muscle regeneration remains unclear. To address this, we analyzed a TNS2-related immunoreactive signal detected with an anti-phospho-TNS2 (Tyr483) antibody during C2C12 myogenic differentiation and in a cardiotoxin (CTX)-induced tibialis anterior (TA) injury model, and assessed regeneration in Tns2 mutant mice. Under basal conditions, Tns2 mice showed no obvious abnormalities in muscle histology, myofiber size, or phosphorylation of Akt and p70 ribosomal protein S6 kinase (p70S6K), a canonical downstream effector of mTORC1 signaling, compared with wild-type (WT) mice, although the soleus muscle/body weight ratio was slightly increased. In C2C12 cells, the anti-phospho-TNS2 (Tyr483)-reactive signal was stronger during proliferation than during differentiation. In vivo, this signal increased transiently during the early phase of CTX-mediated regeneration. After injury, Tns2 mice showed a greater number of centrally nucleated fibers at day 4, larger myofibers at day 7, and a higher TA muscle/body weight ratio at day 15. In addition, phosphorylation of p70S6K was increased in regenerating muscle in Tns2 mice. These findings suggest that TNS2-related signaling may act as a context-dependent modulator of regenerative myofiber growth, possibly through augmented anabolic signaling during early regeneration. |
| title | Tensin2 deficiency transiently accelerates early-phase regenerative myofiber growth following cardiotoxin-induced injury in mice. |
| url | https://pubmed.ncbi.nlm.nih.gov/42161565/ |