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| Format: | Artículo científico |
| Sprache: | en |
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Aquaculture nutrition
2026
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/42164260/ |
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| author | Shi, Huizhong Jiang, Liu Cai, Yan Chen, Zihan Hu, Yongchao Zhou, Yongcan Li, Jianlong Zhang, Dongdong Cao, Zhenjie Wang, Shifeng |
| author_facet | Shi, Huizhong Jiang, Liu Cai, Yan Chen, Zihan Hu, Yongchao Zhou, Yongcan Li, Jianlong Zhang, Dongdong Cao, Zhenjie Wang, Shifeng Shi, Huizhong Jiang, Liu Cai, Yan Chen, Zihan Hu, Yongchao Zhou, Yongcan Li, Jianlong Zhang, Dongdong Cao, Zhenjie Wang, Shifeng |
| collection | PubMed - marine biology |
| contents | Regulatory Effects of a Lipid-Lowering Strain 58 Isolated From Dregs Vinegar on Metabolism-Related Gene Expression, Gut Microbiota, and Metabolic Biomarkers of Hybrid Grouper Under High-Fat Diets. Shi, Huizhong Jiang, Liu Cai, Yan Chen, Zihan Hu, Yongchao Zhou, Yongcan Li, Jianlong Zhang, Dongdong Cao, Zhenjie Wang, Shifeng 58 (lactic acid bacteria [LAB] 58), isolated from vinegar dregs, was incorporated at 1 × 10 CFU/g diet into the feeds of hybrid groupers to assess its lipid-lowering efficacy under normal (7.52 % lipid) or high-fat (14.83 % lipid) regimens. A 2 × 2 factorial design generated four diets: C (normal), H (high-fat), C58 (normal + LAB 58), and H58 (high-fat + LAB 58). Serum biochemical parameters tests, histological section, and Oil Red O staining results indicated that in both LAB 58-supplemented groups (C58 and H58), serum triglycerides and total cholesterol (T-CHO) declined regardless of basal lipid content, and hepatic fat vacuolation and lipid-droplet accumulation were markedly curtailed. The qPCR data revealed that LAB 58 elevated the levels of hepatic lipolysis genes (adipose triglyceride lipase [ATGL], carnitine palmitoyltransferase 1 [CPT-1], farnesoid X receptor [FXR], and lipoprotein lipase [LPL]) while simultaneously suppressing lipid synthesis gene expressions (fatty acid synthase [FAS] and stearoyl-CoA desaturase 1 [SCD-1]) in the H58 group compared with the H group. Gut-microbiota profiling showed a pronounced Firmicutes-to-Bacteroidetes shift, driven by increased Bacteroides, Faecalibacterium, and Lachnospiraceae-UCG-004; the relative abundance of spp. surged, especially in the H58 group. Metabolomic analysis of intestinal contents further disclosed elevated levels of beneficial fatty acids-linoleic and α-linolenic acids in H58 group. In conclusion, these findings demonstrate that LAB 58 alleviates lipid metabolic disorders by modulating hepatic gene expression, restructuring the gut microbiota, and enhancing beneficial metabolites. |
| format | Artículo científico |
| id | pubmed_42164260 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Aquaculture nutrition |
| record_format | pubmed |
| spellingShingle | Regulatory Effects of a Lipid-Lowering Strain 58 Isolated From Dregs Vinegar on Metabolism-Related Gene Expression, Gut Microbiota, and Metabolic Biomarkers of Hybrid Grouper Under High-Fat Diets. Shi, Huizhong Jiang, Liu Cai, Yan Chen, Zihan Hu, Yongchao Zhou, Yongcan Li, Jianlong Zhang, Dongdong Cao, Zhenjie Wang, Shifeng Regulatory Effects of a Lipid-Lowering Strain 58 Isolated From Dregs Vinegar on Metabolism-Related Gene Expression, Gut Microbiota, and Metabolic Biomarkers of Hybrid Grouper Under High-Fat Diets. Shi, Huizhong Jiang, Liu Cai, Yan Chen, Zihan Hu, Yongchao Zhou, Yongcan Li, Jianlong Zhang, Dongdong Cao, Zhenjie Wang, Shifeng 58 (lactic acid bacteria [LAB] 58), isolated from vinegar dregs, was incorporated at 1 × 10 CFU/g diet into the feeds of hybrid groupers to assess its lipid-lowering efficacy under normal (7.52 % lipid) or high-fat (14.83 % lipid) regimens. A 2 × 2 factorial design generated four diets: C (normal), H (high-fat), C58 (normal + LAB 58), and H58 (high-fat + LAB 58). Serum biochemical parameters tests, histological section, and Oil Red O staining results indicated that in both LAB 58-supplemented groups (C58 and H58), serum triglycerides and total cholesterol (T-CHO) declined regardless of basal lipid content, and hepatic fat vacuolation and lipid-droplet accumulation were markedly curtailed. The qPCR data revealed that LAB 58 elevated the levels of hepatic lipolysis genes (adipose triglyceride lipase [ATGL], carnitine palmitoyltransferase 1 [CPT-1], farnesoid X receptor [FXR], and lipoprotein lipase [LPL]) while simultaneously suppressing lipid synthesis gene expressions (fatty acid synthase [FAS] and stearoyl-CoA desaturase 1 [SCD-1]) in the H58 group compared with the H group. Gut-microbiota profiling showed a pronounced Firmicutes-to-Bacteroidetes shift, driven by increased Bacteroides, Faecalibacterium, and Lachnospiraceae-UCG-004; the relative abundance of spp. surged, especially in the H58 group. Metabolomic analysis of intestinal contents further disclosed elevated levels of beneficial fatty acids-linoleic and α-linolenic acids in H58 group. In conclusion, these findings demonstrate that LAB 58 alleviates lipid metabolic disorders by modulating hepatic gene expression, restructuring the gut microbiota, and enhancing beneficial metabolites. |
| title | Regulatory Effects of a Lipid-Lowering Strain 58 Isolated From Dregs Vinegar on Metabolism-Related Gene Expression, Gut Microbiota, and Metabolic Biomarkers of Hybrid Grouper Under High-Fat Diets. |
| url | https://pubmed.ncbi.nlm.nih.gov/42164260/ |