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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Aquaculture nutrition
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/42164260/ |
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Table of Contents:
- Regulatory Effects of a Lipid-Lowering Strain 58 Isolated From Dregs Vinegar on Metabolism-Related Gene Expression, Gut Microbiota, and Metabolic Biomarkers of Hybrid Grouper Under High-Fat Diets. Shi, Huizhong Jiang, Liu Cai, Yan Chen, Zihan Hu, Yongchao Zhou, Yongcan Li, Jianlong Zhang, Dongdong Cao, Zhenjie Wang, Shifeng 58 (lactic acid bacteria [LAB] 58), isolated from vinegar dregs, was incorporated at 1 × 10 CFU/g diet into the feeds of hybrid groupers to assess its lipid-lowering efficacy under normal (7.52 % lipid) or high-fat (14.83 % lipid) regimens. A 2 × 2 factorial design generated four diets: C (normal), H (high-fat), C58 (normal + LAB 58), and H58 (high-fat + LAB 58). Serum biochemical parameters tests, histological section, and Oil Red O staining results indicated that in both LAB 58-supplemented groups (C58 and H58), serum triglycerides and total cholesterol (T-CHO) declined regardless of basal lipid content, and hepatic fat vacuolation and lipid-droplet accumulation were markedly curtailed. The qPCR data revealed that LAB 58 elevated the levels of hepatic lipolysis genes (adipose triglyceride lipase [ATGL], carnitine palmitoyltransferase 1 [CPT-1], farnesoid X receptor [FXR], and lipoprotein lipase [LPL]) while simultaneously suppressing lipid synthesis gene expressions (fatty acid synthase [FAS] and stearoyl-CoA desaturase 1 [SCD-1]) in the H58 group compared with the H group. Gut-microbiota profiling showed a pronounced Firmicutes-to-Bacteroidetes shift, driven by increased Bacteroides, Faecalibacterium, and Lachnospiraceae-UCG-004; the relative abundance of spp. surged, especially in the H58 group. Metabolomic analysis of intestinal contents further disclosed elevated levels of beneficial fatty acids-linoleic and α-linolenic acids in H58 group. In conclusion, these findings demonstrate that LAB 58 alleviates lipid metabolic disorders by modulating hepatic gene expression, restructuring the gut microbiota, and enhancing beneficial metabolites.