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| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
bioRxiv : the preprint server for biology
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/42182328/ |
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| _version_ | 1868266044106211329 |
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| author | Kantarci, Husniye Wu, Kathryn Ambiel, Nicholas Barriera, Eduardo Chaparro Cooper, Madeline H Münch, Alexandra Sigal, Yari M Garcia, Miguel A Iyer, Manasi Jorgens, Danielle M Zuchero, J Bradley |
| author_facet | Kantarci, Husniye Wu, Kathryn Ambiel, Nicholas Barriera, Eduardo Chaparro Cooper, Madeline H Münch, Alexandra Sigal, Yari M Garcia, Miguel A Iyer, Manasi Jorgens, Danielle M Zuchero, J Bradley Kantarci, Husniye Wu, Kathryn Ambiel, Nicholas Barriera, Eduardo Chaparro Cooper, Madeline H Münch, Alexandra Sigal, Yari M Garcia, Miguel A Iyer, Manasi Jorgens, Danielle M Zuchero, J Bradley |
| collection | PubMed - marine biology |
| contents | Actin disassembly triggers CNS myelin compaction and wrapping. Kantarci, Husniye Wu, Kathryn Ambiel, Nicholas Barriera, Eduardo Chaparro Cooper, Madeline H Münch, Alexandra Sigal, Yari M Garcia, Miguel A Iyer, Manasi Jorgens, Danielle M Zuchero, J Bradley Compact myelin enables rapid and precise impulse conduction in the vertebrate nervous system. During CNS development, oligodendrocytes wrap spirally around axons while compacting membranes, yet how cytoskeletal remodeling is coupled to these events remains unclear. Actin disassembly is required for wrapping, but whether wrapping is driven by iterative actin-based protrusion or follows a transition to actin-independent mechanisms is unresolved. Here we integrate tract-resolved developmental profiling, live-cell compaction mapping, and in vivo genetic perturbation to define how actin disassembly is coupled to myelin wrapping. We find that actin filaments undergo pronounced, sustained disassembly before active wrapping begins, with little evidence for persistent actin within sheaths during wrapping. We develop a live-cell assay that maps membrane compaction in cultured oligodendrocytes and show that compaction zones are depleted of actin filaments and expand when actin disassembly is promoted. Consistent with this model, oligodendrocyte-specific actin disassembly in vivo accelerates the appearance of thicker myelin early in development. Together, our results support a model in which actin disassembly promotes myelin wrapping by enabling membrane compaction, and provide a platform to dissect how compaction is regulated in development and disease. |
| format | Artículo científico |
| id | pubmed_42182328 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | bioRxiv : the preprint server for biology |
| record_format | pubmed |
| spellingShingle | Actin disassembly triggers CNS myelin compaction and wrapping. Kantarci, Husniye Wu, Kathryn Ambiel, Nicholas Barriera, Eduardo Chaparro Cooper, Madeline H Münch, Alexandra Sigal, Yari M Garcia, Miguel A Iyer, Manasi Jorgens, Danielle M Zuchero, J Bradley Actin disassembly triggers CNS myelin compaction and wrapping. Kantarci, Husniye Wu, Kathryn Ambiel, Nicholas Barriera, Eduardo Chaparro Cooper, Madeline H Münch, Alexandra Sigal, Yari M Garcia, Miguel A Iyer, Manasi Jorgens, Danielle M Zuchero, J Bradley Compact myelin enables rapid and precise impulse conduction in the vertebrate nervous system. During CNS development, oligodendrocytes wrap spirally around axons while compacting membranes, yet how cytoskeletal remodeling is coupled to these events remains unclear. Actin disassembly is required for wrapping, but whether wrapping is driven by iterative actin-based protrusion or follows a transition to actin-independent mechanisms is unresolved. Here we integrate tract-resolved developmental profiling, live-cell compaction mapping, and in vivo genetic perturbation to define how actin disassembly is coupled to myelin wrapping. We find that actin filaments undergo pronounced, sustained disassembly before active wrapping begins, with little evidence for persistent actin within sheaths during wrapping. We develop a live-cell assay that maps membrane compaction in cultured oligodendrocytes and show that compaction zones are depleted of actin filaments and expand when actin disassembly is promoted. Consistent with this model, oligodendrocyte-specific actin disassembly in vivo accelerates the appearance of thicker myelin early in development. Together, our results support a model in which actin disassembly promotes myelin wrapping by enabling membrane compaction, and provide a platform to dissect how compaction is regulated in development and disease. |
| title | Actin disassembly triggers CNS myelin compaction and wrapping. |
| url | https://pubmed.ncbi.nlm.nih.gov/42182328/ |