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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Proceedings of the National Academy of Sciences of the United States of America
2026
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| Materias: | |
| Acceso en línea: | https://pubmed.ncbi.nlm.nih.gov/42224606/ |
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| _version_ | 1868266041742721026 |
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| author | Zheng, Rikuan Wang, Chong Zheng, Yuxin Cao, Lei Liu, Kaitao Li, Jie Sun, Chaomin |
| author_facet | Zheng, Rikuan Wang, Chong Zheng, Yuxin Cao, Lei Liu, Kaitao Li, Jie Sun, Chaomin Zheng, Rikuan Wang, Chong Zheng, Yuxin Cao, Lei Liu, Kaitao Li, Jie Sun, Chaomin |
| collection | PubMed - marine biology |
| contents | Polyphosphate affects the growth and methanogenesis of deep-sea methanogenic archaea. Zheng, Rikuan Wang, Chong Zheng, Yuxin Cao, Lei Liu, Kaitao Li, Jie Sun, Chaomin Polyphosphates Methane Archaea Seawater Phosphotransferases (Phosphate Group Acceptor) Archaeal Proteins Gene Expression Regulation, Archaeal Geologic Sediments Microorganisms employ inorganic polyphosphate (polyP) as an ancient strategy for energy and phosphate storage, yet its physiological roles in methanogenic archaea remain largely unexplored. Here, we report that polyP metabolism is coupled to growth and methanogenesis in sp. ZRKC1, a representative of eight methanogenic archaea isolated from deep-sea cold seep sediments. Through combined genetic, biochemical, and physiological analyses, we find that PPK1 mediates polyP synthesis in a Mg-dependent manner, whereas PPK2 functions primarily as a polyP hydrolase. Deletion of in strain ZRKC1 abolishes polyP accumulation and impairs both growth and methane production, pointing to a role for polyP as a metabolic hub linking these processes. Transcriptomic profiling reveals that under organic phosphorus conditions, strain ZRKC1 upregulates phosphoesterases to liberate bioavailable phosphate, which is subsequently channeled into polyP via PPK1. Furthermore, in situ transcriptomic data suggest that the genetic capacity for this metabolic strategy may be present and transcriptionally active in the native environment, with concurrent upregulation of genes involved in phosphate acquisition, polyP metabolism, and methanogenesis. Our findings suggest the importance of polyP in linking phosphate homeostasis to growth and methanogenesis in deep-sea methanogenic archaea. |
| format | Artículo científico |
| id | pubmed_42224606 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Proceedings of the National Academy of Sciences of the United States of America |
| record_format | pubmed |
| spellingShingle | Polyphosphate affects the growth and methanogenesis of deep-sea methanogenic archaea. Zheng, Rikuan Wang, Chong Zheng, Yuxin Cao, Lei Liu, Kaitao Li, Jie Sun, Chaomin Polyphosphates Methane Archaea Seawater Phosphotransferases (Phosphate Group Acceptor) Archaeal Proteins Gene Expression Regulation, Archaeal Geologic Sediments Polyphosphate affects the growth and methanogenesis of deep-sea methanogenic archaea. Zheng, Rikuan Wang, Chong Zheng, Yuxin Cao, Lei Liu, Kaitao Li, Jie Sun, Chaomin Polyphosphates Methane Archaea Seawater Phosphotransferases (Phosphate Group Acceptor) Archaeal Proteins Gene Expression Regulation, Archaeal Geologic Sediments Microorganisms employ inorganic polyphosphate (polyP) as an ancient strategy for energy and phosphate storage, yet its physiological roles in methanogenic archaea remain largely unexplored. Here, we report that polyP metabolism is coupled to growth and methanogenesis in sp. ZRKC1, a representative of eight methanogenic archaea isolated from deep-sea cold seep sediments. Through combined genetic, biochemical, and physiological analyses, we find that PPK1 mediates polyP synthesis in a Mg-dependent manner, whereas PPK2 functions primarily as a polyP hydrolase. Deletion of in strain ZRKC1 abolishes polyP accumulation and impairs both growth and methane production, pointing to a role for polyP as a metabolic hub linking these processes. Transcriptomic profiling reveals that under organic phosphorus conditions, strain ZRKC1 upregulates phosphoesterases to liberate bioavailable phosphate, which is subsequently channeled into polyP via PPK1. Furthermore, in situ transcriptomic data suggest that the genetic capacity for this metabolic strategy may be present and transcriptionally active in the native environment, with concurrent upregulation of genes involved in phosphate acquisition, polyP metabolism, and methanogenesis. Our findings suggest the importance of polyP in linking phosphate homeostasis to growth and methanogenesis in deep-sea methanogenic archaea. |
| title | Polyphosphate affects the growth and methanogenesis of deep-sea methanogenic archaea. |
| topic | Polyphosphates Methane Archaea Seawater Phosphotransferases (Phosphate Group Acceptor) Archaeal Proteins Gene Expression Regulation, Archaeal Geologic Sediments |
| url | https://pubmed.ncbi.nlm.nih.gov/42224606/ |